Journal of Mushroom (한국버섯학회지)

The Korean Society of Mushroom Science (한국버섯학회)
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Characteristics of the protein and antioxidant contents in soybeans cultured with Phellinus linteus HN00K9

목질진흙버섯 (Phellinus linteus) HN00K9 균사체 배양 대두의 단백질함량 및 항산화활성 특성

  • Ja-Yoon Kim (School of Biotechnology, Division of Horticultural Biotechnology, Hankyong National University) ;
  • Ye-Lin Baek (School of Biotechnology, Division of Horticultural Biotechnology, Hankyong National University) ;
  • Sang-Ho Lee (Mythings Co., Ltd.) ;
  • Hee-Wan Kang (School of Biotechnology, Division of Horticultural Biotechnology, Hankyong National University)
  • 김자윤 (한경국립대학교 생명공학부) ;
  • 백예린 (한경국립대학교 생명공학부) ;
  • 이상호 ((주) 마이띵스 ) ;
  • 강희완 (한경국립대학교 생명공학부)
  • Received : 2023.08.14
  • Accepted : 2023.09.15
  • Published : 2023.09.30
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Abstract

In this study, the protein content and functional changes in soybeans cultured with Phellinus linteus HN00K9 were analyzed. P. linteus HN00K9 was cultured on soybeans. The crude protein content in soybeans cultured with HN00K9 (PMS) was 41.99%, which was higher than that in soybeans not cultured with the mushroom (UCS). The total free amino acid content in PMS increased to 39,963 mg/100 g, which was higher than that in UCS (36,817 mg/100 g). In particular, in PMS, glutamic acid accounted for 18.5% of the total amino acids at 7,413 mg/100 g. The total polyphenol content in PMS was 2.66 mg GAE/g, which was more than 45% higher than the amount in UCS (1.45 mg GAE/g). Additionally, PMS showed a DPPH radical scavenging activity of 33.3%, which was 3 times higher than that exhibited by UCS (11.5%), reflecting its high antioxidant content. Therefore, the PMS in this study has potential for use as a functional food material.

목질진흙버섯 HN00K9균사체 액체종균을 대두에 배양한 대두(PMS)의 조단백질 함량은 41.99%로 HN00K9 균사체 배양하지 않은 대두(UCS) 38.61%보다 높은 단백질 함량이 검출되었다. PMS와 UCS에서 16종의 유리 아미노산이 검출되었으며 총 아미노산함량은 PMS에서 39,963 mg/100 g으로 UCS의 36,817 mg/100 g보다 8% 이상 증가되었다. 특히 glutamic acid는 PMS에서 7,413 mg/100 g로 전체 아미노산 중 가장 많은 18.5%로 나타났다. 총 polyphenol 함량은 PMS에서 2.66 mg GAE/g로 UCS 1.45 mg GAE/g보다 45%이상 높게 검출되었으며 PMS는 33.3%의 DPPH radical 소거 능을 보여 UCS의 11.5%보다 3배 증가한 높은 항산화 활성을 보였다. 따라서 본 연구의 PMS는 높은 단백질함량과 항산화 활성이 강화된 기능성 식품소재로 활용할 수 있을 것으로 기대된다.

Keywords

Acknowledgement

본 연구는 과학기술정보통신부 연구개발특구진흥재단 민간투자형 R&BD (과제번호: 2022-DD-RD-0574-02) 지원으로 수행된 연구결과이며 연구비 지원에 감사드립니다.

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