대한본초학회지 (The Korea Journal of Herbology)

  • 제38권5호
  • /
  • Pages.9-19
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  • 2023
  • /
  • 1229-1765(pISSN)
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  • 2288-7199(eISSN)
대한본초학회 (The Korea Association of Herbology)
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백렴(白蘞)의 파골세포 분화 및 관련 유전자 발현 억제에 미치는 영향

The Effect of Ampelopsis japonica (Thunb.) Makino on Osteoclastogenesis and Expression of Osteoclast-Related Gene

  • 김홍식 (경희대학교 한의과대학 해부학교실) ;
  • 이수민 (경희대학교 한의과대학 해부학교실) ;
  • 김민선 (경희대학교 한의과대학 해부학교실) ;
  • 김재현 (경희대학교 한의과대학 해부학교실) ;
  • 강예진 (경희대학교 한의과대학 해부학교실) ;
  • 권성준 (경희대학교 한의과대학 해부학교실) ;
  • 남영우 (경희대학교 한의과대학 해부학교실) ;
  • 유승우 (경희대학교 한의과대학 해부학교실) ;
  • 최홍석 (경희대학교 한의과대학 해부학교실) ;
  • 허선진 (경희대학교 한의과대학 해부학교실) ;
  • 손영주 (경희대학교 한의과대학 해부학교실) ;
  • 정혁상 (경희대학교 한의과대학 해부학교실)
  • Hongsik Kim (Department of Anatomy, College of Korean Medicine, Kyung Hee University) ;
  • Sumin Lee (Department of Anatomy, College of Korean Medicine, Kyung Hee University) ;
  • Minsun Kim (Department of Anatomy, College of Korean Medicine, Kyung Hee University) ;
  • Jae-Hyun Kim (Department of Anatomy, College of Korean Medicine, Kyung Hee University) ;
  • Yejin Kang (Department of Anatomy, College of Korean Medicine, Kyung Hee University) ;
  • Seoung Jun Kwon (Department of Anatomy, College of Korean Medicine, Kyung Hee University) ;
  • Youngwoo Nam (Department of Anatomy, College of Korean Medicine, Kyung Hee University) ;
  • Seungwoo Yoo (Department of Anatomy, College of Korean Medicine, Kyung Hee University) ;
  • Hong-Seok Choi (Department of Anatomy, College of Korean Medicine, Kyung Hee University) ;
  • SeonJin Huh (Department of Anatomy, College of Korean Medicine, Kyung Hee University) ;
  • Youngjoo Sohn (Department of Anatomy, College of Korean Medicine, Kyung Hee University) ;
  • Hyuk-Sang Jung (Department of Anatomy, College of Korean Medicine, Kyung Hee University)
  • 투고 : 2023.07.27
  • 심사 : 2023.09.25
  • 발행 : 2023.09.30
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초록

Objectives : Osteoporosis is a systemic skeletal disorder characterized by reduced bone mineral density and increased risk of fractures. Bisphosphonates and selective estrogen receptors, which are bone resorption inhibitors that are currently widely used as osteoporosis treatments, show serious side effects when administered for a long time. Research on bone resorption inhibitors that complement the problems of existing treatments is needed. The purpose of this study was to investigate the effect of inhibiting osteoclast differentiation and activity on the tuberous root of Ampelopsis japonica (Thunb.) Makino (AM). Methods : After extracting AM using distilled water and ethanol, the inhibitory effects of the two solvents on osteoclast differentiation were compared using the RANKL-induced in vitro experimental model and the TRAP assay kit. The impact of AM on bone resorption was investigated through the pit formation assay, and its effect on F-actin formation was assessed through fluorescent staining. Additionally, protein and mRNA expression levels of osteoclast differentiation markers (NFATc1, c-Fos, TRAP and ATP6v0d2) and resorption markers (MMP-9, CTK, and CA2) were analyzed via western blot and RT-PCR. Results : AM treatment significantly decreased the number of TRAP-positive cells and pit formation area. Furthermore, AM suppressed both the protein and mRNA expression of NFATc1 and c-Fos, key transcription factors involved in osteoclast differentiation and it downregulated the expression of osteoclast-associated genes such as TRAP, CTK, MMP-9, CA2, and ATP6v0d2. Conclusions : These results suggest that AM can inhibit bone resorption and osteoclast differentiation, indicating its potential for use in the treatment and prevention of osteoporosis.

키워드

과제정보

이 논문은 2021년도 정부 (보건복지부)의 재원으로 한국보건산업진흥원의 지원을 받아 수행된 연구임 (No. HF21C0092).

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