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Identification of a norovirus from diarrheic dog in Gwangju, Republic of Korea

  • Ba-Ra-Da Koh (Veterinary Service Laboratory, Gwangju Metropolitan City Health & Environment Research institute) ;
  • Su-Yeon Seo (Veterinary Service Laboratory, Gwangju Metropolitan City Health & Environment Research institute) ;
  • Ga-Hoi Choi (Veterinary Service Laboratory, Gwangju Metropolitan City Health & Environment Research institute) ;
  • Byeong-Cheol Yoon (Veterinary Service Laboratory, Gwangju Metropolitan City Health & Environment Research institute)
  • Received : 2023.08.02
  • Accepted : 2023.09.13
  • Published : 2023.09.30

Abstract

Noroviruses are a major cause of gastroenteritis in humans and animals worldwide. In 2021, canine norovirus (CNoV) infection was detected at an animal clinic in Gwangju area, South Korea. A semi-nested polymerase chain reaction was developed to amplify a 478 bp fragment of the RdRp gene of CNoV. The phylogenetic analysis of this fragment confirmed the strain to be genogroup IV.2 (Dog/GIV.2/gw/s377/2021/KOR), which exhibited the highest similarity to the feline NoV strain GIV.2/CU081210E/USA/2010 (accession no. NC_045762) with 95.1% nucleotide (nt) identity and 98.7% amino acid (aa) identity. These research findings indicate that the detected norovirus in dogs is genetically similar to a feline-origin norovirus, suggesting easy cross-species transmission among animals.

Keywords

Acknowledgement

This work was supported by Gwangju Metropolitan City Institute of Health & Environment's Research Capacity Enhancement Project in 2022.

References

  1. Bodnar L, Lorusso E, Di Martino B, Catella C, Lanave G, Elia G, Banyai K, Buonavoglia C, Martella V. 2017. Identification of a novel canine norovirus. Infect Genet Evol 52: 75-81. https://doi.org/10.1016/j.meegid.2017.04.020
  2. Chan MCW, Shan Kwan H, Chan PKS. 2017. Structure and genotypes of noroviruses. The Norovirus 51-63.
  3. Chhabra P, de Graaf M, Parra GI, Chan MC, Green K, Martella V, Wang Q, White PA, Katayama K, Vennema H, Koopmans MPG, Vinje J. 2019. Updated classification of norovirus genogroups and genotypes. J Gen Virol 100: 1393-1406. https://doi.org/10.1099/jgv.0.001318
  4. Di Martino B, Di Profio F, Ceci C, Di Felice E, Green KY, Bok K, De Grazia S, Giammanco GM, Massirio I, Lorusso E, Buonavoglia C, Marsilio F, Martella V. 2014. Seroprevalence of norovirus genogroup IV antibodies among humans, Italy, 2010-2011. Emerg Infect Dis 20: 1828-1832. https://doi.org/10.3201/eid2011.131601
  5. Di Martino B, Di Profio F, Melegari I, Sarchese V, Cafiero MA, Robetto S, Aste G, Lanave G, Marsilio F, Martella V. 2016. A novel feline norovirus in diarrheic cats. Infect Genet Evol 38: 132-137. https://doi.org/10.1016/j.meegid.2015.12.019
  6. Di Martino B, Di Profio F, Melegari I, Sarchese V, Massirio I, Palermo G, Romito G, Lorusso E, Lanave G, Bodnar L, Buonavoglia C, Marsilio F, Green KY, Martella V. 2017. Seroprevalence for norovirus genogroup II, IV and VI in dogs. Vet Microbiol 203: 68-72. https://doi.org/10.1016/j.vetmic.2017.03.006
  7. Ford-Siltz LA, Mullis L, Sanad YM, Tohma K, Lepore CJ, Azevedo M, Parra GI. 2019. Genomics analyses of GIV and GVI noroviruses reveal the distinct clustering of human and animal viruses. Viruses 11: 204.
  8. Han TH, Kim SC, Kim ST, Chung CH, Chung JY. 2014. Detection of norovirus genogroup IV, klassevirus, and pepper mild mottle virus in sewage samples in South Korea. Arch Virol 159: 457-463. https://doi.org/10.1007/s00705-013-1848-7
  9. Lyoo KS, Jung MC, Yoon SW, Kim HK, Jeong DG. 2018. Identification of canine norovirus in dogs in South Korea. BMC Vet Res 14: 413.
  10. Ma H, Yue H, Luo Y, Li S, Tang C. 2021. First detection of canine norovirus in dogs and a complete GVI.2 genome in mainland China. Infect Genet Evol 92: 104879.
  11. Martella V, Campolo M, Lorusso E, Cavicchio P, Camero M, Bellacicco AL, Decaro N, Elia G, Greco G, Corrente M, Desario C, Arista S, Banyai K, Koopmans M, Buonavoglia C. 2007. Norovirus in captive lion cub (Panthera leo). Emerg Infect Dis 13: 1071-1073. https://doi.org/10.3201/eid1307.070268
  12. Martella V, Lorusso E, Decaro N, Elia G, Radogna A, D'Abramo M, Desario C, Cavalli A, Corrente M, Camero M, Germinario CA, Banyai K, Di Martino B, Marsilio F, Carmichael LE, Buonavoglia C. 2008. Detection and molecular characterization of a canine norovirus. Emerg Infect Dis 14: 1306-1308. https://doi.org/10.3201/eid1408.080062
  13. Mesquita JR, Barclay L, Nascimento MS, Vinje J. 2010. Novel norovirus in dogs with diarrhea. Emerg Infect Dis 16: 980-982. https://doi.org/10.3201/eid1606.091861
  14. Mesquita JR, Delgado I, Costantini V, Heenemann K, Vahlenkamp TW, Vinje J, Nascimento MS. 2014. Seroprevalence of canine norovirus in 14 European countries. Clin Vaccine Immunol 21: 898-900. https://doi.org/10.1128/CVI.00048-14
  15. Ntafis V, Xylouri E, Radogna A, Buonavoglia C, Martella V. 2010. Outbreak of canine norovirus infection in young dogs. J Clin Microbiol 48: 2605-2608. https://doi.org/10.1128/JCM.02528-09
  16. Pinto P, Wang Q, Chen N, Dubovi EJ, Daniels JB, Millward LM, Buonavoglia C, Martella V, Saif LJ. 2012. Discovery and genomic characterization of noroviruses from a gastroenteritis outbreak in domestic cats in the US. PLoS One 7: e32739.
  17. Soma T, Nakagomi O, Nakagomi T, Mochizuki M. 2015. Detection of norovirus and sapovirus from diarrheic dogs and cats in Japan. Microbiol Immunol 59:123-128. https://doi.org/10.1111/1348-0421.12223
  18. Stals A, Mathijs E, Baert L, Botteldoorn N, Denayer S, Mauroy A, Scipioni A, Daube G, Dierick K, Herman L, Van Coillie E, Thiry E, Uyttendaele M. 2012. Molecular detection and genotyping of noroviruses. Food Environ Virol 4: 153-167. https://doi.org/10.1007/s12560-012-9092-y
  19. Tamura K, Stecher G, Kumar S. 2021. MEGA 11: molecular evolutionary genetics analysis version 11. Mol Biol Evol 38: 3022-3027. https://doi.org/10.1093/molbev/msab120
  20. Tse H, Lau SK, Chan WM, Choi GK, Woo PC, Yuen KY. 2012. Complete genome sequences of novel canine noroviruses in Hong Kong. J Virol 86: 9531-9532. https://doi.org/10.1128/JVI.01312-12