Korean Journal of Environmental Agriculture (한국환경농학회지)

The Korean Society of Environmental Agriculture (한국환경농학회)
권호 표지
DOI QR코드

DOI QR Code

Survey of Antibiotic Resistant Bacteria in Lake Gyeongpo, Korea

경포호의 항생제 내성 세균 조사

  • Dukki Han (Department of Marine Bioscience, College of Life Sciences, Gangneung-Wonju National University)
  • 한덕기 (강릉원주대학교 해양생명과학과)
  • Received : 2023.06.28
  • Accepted : 2023.08.04
  • Published : 2023.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

The emergence and spread of antibiotic-resistant bacteria have been increasing with anthropogenic contamination. Understanding the prevalence and distribution of these resistant bacteria in environments is crucial for effectively managing anthropogenic pollutants. Lake Gyeongpo in the Gangwon Province of South Korea is known for its diverse ecological features and human interactions. The lake is exposed to pollutants from nonpoint sources, including urban areas, agricultural practices, and recreational activities, which can introduce antibiotics and foster antibiotic resistance in bacteria. The present study investigates Lake Gyeongpo as a potential reservoir for antibiotic-resistant bacteria in a natural ecosystem. A total of 203 bacterial isolates were collected from six sampling locations in Lake Gyeongpo during May, July, and November 2022. Most isolates were taxonomically identified as Pseudoalteromonas, Bacillus, Shewanella, and Vibrio spp.; their abundance showed a spatiotemporal distribution. An antibiotic susceptibility test was conducted on 75 isolates using the disk diffusion method with six drugs according to the CLSI guideline; 42 isolates were resistant to one or more antibiotics. Among these, 15 isolates were identified as multidrug resistant bacteria. This finding suggests the potential anthropogenic impact on Lake Gyeongpo and provides valuable insights into the dissemination of antibiotic resistance caused by anthropogenic pollutants.

Keywords

Acknowledgement

This paper was supported by research funds for newly appointed professors of Gangneung-Wonju National University in 2021 and by Korea Institute of Marine Science & Technology Promotion (KIMST) funded by the Ministry of Oceans and Fisheries (20220533).

References

  1. Kjerfve B (1994) Coastal lagoons. Elsevier Oceanography Series, 60, 1-8. https://doi.org/10.1016/S0422-9894(08)70006-0. 
  2. Kwak S, Bhattrai BD, Choi K, Heo W (2015) Long-term variations of water quality parameters in Lake Kyoungpo. Korean Journal of Ecology and Environment, 48(2), 95-107. https://doi.org/10.11614/KSL.2015.48.2.095. 
  3. Park S, Jang Y, Lee K, Heo W, Cho K, Choi J (2014) Analysis of fish community of lagoons in the East seashore according to hydrach succession. Korean Journal of Ecology and Environment, 47(spc), 83-99. https://doi.org/10.11614/KSL.2014.47(S).083. 
  4. Kwon D, Park M, Lee CS, Park C, Lee SD (2021) New records of the diatoms (Bacillariophyceae) from the Coastal Lagoons in Korea. Journal of Marine Science and Engineering, 9(7), 694. https://doi.org/10.3390/jmse9070694. 
  5. Kim JH, Park CK, Kim MY, Ahn SG (2008) Contamination of veterinary antibiotics and antimicrobials in Han river basin. Journal of the Korean Society for Environmental Analysis, 11, 109-118. 
  6. Hong Y, Lee I, Tae B, Lee W, Pan SY, Snyder SW, Kim H (2021) Contribution of sewage to occurrence of phosphodiesterase-5 inhibitors in natural water. Scientific Reports, 11(1), 1-12. https://doi.org/10.1038/s41598-021-89028-3. 
  7. Lee SH, Jung HW, Jung JY, Min HJ, Kim BR, Park CG, Oh JE, Onoda Y, Satou N (2013) Characteristics of occurrence of pharmaceuticals in the Nakdong river. Journal of Korean Society of Environmental Engineers, 35(1), 45-56.  https://doi.org/10.4491/KSEE.2013.35.1.045
  8. Rai PK, Tripathi B (2007) Microbial contamination in vegetables due to irrigation with partially treated municipal wastewater in a tropical city. International Journal of Environmental Health Research, 17(5), 389-395. https://doi.org/10.1080/09603120701628743. 
  9. Pant A, Mittal AK (2007) Monitoring of pathogenicity of effluents from the UASB based sewage treatment plant. Environmental Monitoring and Assessment, 133(1), 43-51. https://doi.org/10.1007/s10661-006-9558-1. 
  10. Igbinosa EO, Obi LC, Okoh AI (2009) Occurrence of potentially pathogenic vibrios in final effluents of a wastewater treatment facility in a rural community of the Eastern Cape Province of South Africa. Research in Microbiology, 160(8), 531-537. https://doi.org/10.1016/j.resmic.2009.08.007. 
  11. DeVries SL, Zhang P (2016) Antibiotics and the terrestrial nitrogen cycle: A review. Current Pollution Reports, 2(1), 51-67. https://doi.org/10.1007/s40726-016-0027-3. 
  12. Schar D, Klein EY, Laxminarayan R, Gilbert M, Van Boeckel TP (2020) Global trends in antimicrobial use in aquaculture. Scientific Reports, 10(1), 1-9. https://doi.org/10.1038/s41598-020-78849-3. 
  13. Van Boeckel TP, Brower C, Gilbert M, Grenfell BT, Levin SA, Robinson TP, Teillant A, Laxminarayan R (2015) Global trends in antimicrobial use in food animals. Proceedings of the National Academy of Sciences, 112(18), 5649-5654. https://doi.org/10.1073/pnas.1503141112. 
  14. Lee JH, Hong HW, Han DK (2022) Survey of antibiotic resistant bacteria in Ulleungdo, Korea. Korean Journal of Environmental Agriculture, 41(4), 344-354. https://doi.org/10.5338/KJEA.2022.41.4.40. 
  15. Baker-Austin C, Trinanes J, Gonzalez-Escalona N, Martinez-Urtaza J (2017) Non-cholera vibrios: The microbial barometer of climate change. Trends in microbiology, 25(1), 76-84. https://doi.org/10.1016/j.tim.2016.09.008. 
  16. Gauthier G, Gauthier M, Christen R (1995) Phylogenetic analysis of the genera Alteromonas, Shewanella, and Moritella using genes coding for small-subunit rRNA sequences and division of the genus Alteromonas into two genera, Alteromonas (emended) and Pseudoalteromonas gen. nov., and proposal of twelve new species combinations. International Journal of Systematic and Evolutionary Microbiology, 45 (4), 755-761. https://doi.org/10.1099/00207713-45-4-755. 
  17. Satomi M, Oikawa H, Yano Y (2003) Shewanella marinintestina sp. nov., Shewanella schlegeliana sp. nov. and Shewanella sairae sp. nov., novel eicosapentaenoic-acid-producing marine bacteria isolated from sea-animal intestines. International Journal of Systematic and Evolutionary Microbiology, 53(2), 491-499. https://doi.org/10.1099/ijs.0.02392-0. 
  18. Rampelotto PH (2010) Resistance of microorganisms to extreme environmental conditions and its contribution to astrobiology. Sustainability, 2(6), 1602-1623. https://doi.org/10.3390/su2061602. 
  19. Mondol MA., Shin HJ, Islam MT (2013) Diversity of secondary metabolites from marine Bacillus species: Chemistry and biological activity. Marine Drugs, 11 (8), 2846-2872. https://doi.org/10.3390/md11082846.