Journal of the korean academy of Pediatric Dentistry (대한소아치과학회지)

Korean Academy of Pediatric Dentistry (대한소아치과학회)
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Effect of Polydeoxyribonucleotide on Human Periodontal Ligament Cells as a Storage Medium for Avulsed Tooth

탈구치 저장 매체로서 치주인대 세포에 미치는 Polydeoxyribonucleotide의 효과에 대한 연구

  • Sang Tae Ro (Department of Pediatric Dentistry, Kyung Hee University Dental Hospital) ;
  • Yong Kwon Chae (Department of Pediatric Dentistry, Kyung Hee University Dental Hospital) ;
  • Ko Eun Lee (Department of Pediatric Dentistry, Kyung Hee University Dental Hospital) ;
  • Mi Sun Kim (Department of Pediatric Dentistry, School of Dentistry, Kyung Hee University) ;
  • Ok Hyung Nam (Department of Pediatric Dentistry, Kyung Hee University Dental Hospital) ;
  • Hyoseol Lee (Department of Pediatric Dentistry, Kyung Hee University Dental Hospital) ;
  • Sung Chul Choi (Department of Pediatric Dentistry, Kyung Hee University Dental Hospital)
  • 노상태 (경희대학교 치과병원 소아치과) ;
  • 채용권 (경희대학교 치과병원 소아치과) ;
  • 이고은 (경희대학교 치과병원 소아치과) ;
  • 김미선 (경희대학교 치의학전문대학원 소아치과학교실) ;
  • 남옥형 (경희대학교 치과병원 소아치과) ;
  • 이효설 (경희대학교 치과병원 소아치과) ;
  • 최성철 (경희대학교 치과병원 소아치과)
  • Received : 2023.06.23
  • Accepted : 2023.08.02
  • Published : 2023.08.31
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Abstract

Objective: This study aimed to evaluate the suitability of polydeoxyribonucleotides (PDRN) as a storage medium for avulsed teeth. Materials and Methods: The viability of human periodontal ligament (PDL) cells stored in Hank's balanced salt solution and PDRN solutions (concentrations, 10, 25, 50, and 100 ㎍/mL) and tap water was measured using the Cell Counting Kit-8 and Live/Dead assays. In addition, Nitric oxide detection and quantitative real-time polymerase chain reaction (qRT-PCR) were performed to evaluate the anti-inflammatory effect of PDRN. Results: The viability of PDL cells stored in a 100 ㎍/mL PDRN solution was significantly higher than that of cells stored in the other solutions (p < 0.01). Furthermore, cells stored in 100 ㎍/mL PDRN solution demonstrated a significantly reduced NO production (p < 0.0001), and cells stored in 50 and 100 ㎍/mL PDRN solutions expressed significantly lower levels of tumor necrosis factor α, interleukin (IL) -4, IL-6, and IL-10 (p < 0.01) compared to cells stored in HBSS. Conclusion: The PDRN solution exhibited cell-preserving and anti-inflammatory effects on the PDL cells. The findings of this study can serve as a basis for further experiments directed at the development of an effective storage medium for avulsed teeth.

본 연구는 탈구된 치아의 저장 매체로서 polydeoxyribonucleotide (PDRN)의 적합성을 평가하고자 하였다. HBSS와 10, 25, 50, 100 ㎍/mL 농도의 PDRN 용액과 수돗물에 저장된 인간 치주인대 세포의 생존율을 측정하기 위해 Cell Counting Kit-8 assay와 Live/Dead assay를 시행하였다. 또한, PDRN의 항염증 효과를 평가하기 위한 NO 검출 및 qRT-PCR 실험을 진행하였다. 100 ㎍/mL 농도의 PDRN 용액에 저장된 치주인대 세포의 생존율이 다른 용액보다 유의하게 높았다(p < 0.01). 또한, 100 ㎍/mL 농도의 PDRN 용액은 유의하게 NO의 생산을 줄였다(p < 0.0001). 그리고, HBSS 용액에 비하여 50 및 100 ㎍/mL 농도의 PDRN 용액에서 유의하게 tumor necrosis factor α, interleukin (IL)-4, IL-6, 그리고 IL-10의 발현이 낮았다(p < 0.01). 이 연구를 통해 PDRN은 치주인대 세포에 세포 보존 및 항염증 효과를 가진 것으로 밝혀졌다. 이 연구는 효과적인 탈구치 저장매체의 개발을 위한 향후 추가적인 실험의 기반이 될 수 있을 것이라 생각한다.

Keywords

Acknowledgement

This research was supported by the Basic Science Research Program through the National Research Foundation of Korea funded by the Ministry of Education, Science and Technology (grant no. NRF-2016R1D1A1B03931063).

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