Journal of the korean academy of Pediatric Dentistry (대한소아치과학회지)

Korean Academy of Pediatric Dentistry (대한소아치과학회)
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An in Vitro Study of the Effects of Different Dentin Bonding Agents on the Prevention of Tooth Discoloration and the Sealing Ability of Calcium Silicate-Based Cement in Regenerative Endodontic Procedures

치수재혈관화 술식에서 다양한 상아질 접착제가 치아 변색과 칼슘 실리케이트 시멘트의 밀폐 효과에 미치는 영향에 대한 실험실적 연구

  • Jieun Han (Department of Pediatric Dentistry, School of Dentistry, Kyungpook National University) ;
  • Gimin Kim (Department of Pediatric Dentistry, School of Dentistry, Kyungpook National University) ;
  • Jaesik Lee (Department of Pediatric Dentistry, School of Dentistry, Kyungpook National University)
  • 한지은 (경북대학교 치과대학 소아치과학교실) ;
  • 김기민 (경북대학교 치과대학 소아치과학교실) ;
  • 이제식 (경북대학교 치과대학 소아치과학교실)
  • Received : 2023.05.07
  • Accepted : 2023.06.27
  • Published : 2023.08.31
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Abstract

This study aimed to compare the effects of applying different types of dentin bonding agents (DBAs) on tooth and on the sealing ability of CSC in regenerative endodontic procedures (REPs). Specimens were made from 120 human first premolars and categorized into the following groups based on the presence and the type of DBA: Group I, no bonding; Group II, One-Step; Group III, Clearfil SE Bond; and Group IV, Single Bond Universal. Subsequently, the groups were categorized into Subgroup a and Subgroup b, in which Endocem MTA® Premixed Regular and Biodentine were used, respectively. The CIE L*a*b* on the photos taken prior to TAP placement (S0), 1 week after TAP placement (S1), 2 weeks after TAP placement (S2), 4 weeks after TAP placement (S3) and 2 weeks after CSC placement (S4) was used to assess discoloration. The samples were submerged in a 2% methylene blue solution and magnified by 30 times under a stereomicroscope to assess microleakage. The application of DBA during REP reduced crown discoloration, while Biodentine showed minimal discoloration with or without DBA and regardless of the DBA type. Additionally, the application of DBA did not increase microleakage.

본 연구는 치수 재혈관화 과정 중 상아질 접착제 도포 유무 및 종류가 치아 변색과 미세 누출에 미치는 영향을 평가하고자 하였다. 시편은 사람의 발거된 소구치 120개로 제작하였으며, 상아질 접착제 종류에 따라 다음과 같이 분류하였다. Group I. No bonding; Group II. One-Step; Group III. Clearfil SE Bond; Group IV. Single Bond Universal. 각 그룹은 칼슘 규산염계 시멘트인 Endocem MTA® Premixed Regular와 Biodentine의 사용에 따라 Subgroup a와 Subgroup b로 나뉘어 총 8개의 그룹으로 분류되었다. 치관변색 평가를 위해 초진(S0), 항생제 도포 1주(S1), 2주(S2), 4주 경과(S3)와 칼슘 규산염계 시멘트 적용 2주 경과(S4) 각 시점에서 사진을 촬영해 CIE L*a*b*를 측정하였다. 모든 시편은 2% 메틸렌 블루 용액에 침적 후 30배율로 관찰하여 미세누출을 평가하였다. 상아질 접착제 도포는 치관 변색을 감소시켰고, Biodentine은 상아질 접착제의 도포 유무 및 종류와 상관없이 최소한의 변색을 보였다. 또한 상아질 접착제 도포는 미세 누출을 증가시키지 않았다.

Keywords

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