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An in Vitro Study of the Effects of Different Dentin Bonding Agents on the Prevention of Tooth Discoloration and the Sealing Ability of Calcium Silicate-Based Cement in Regenerative Endodontic Procedures

치수재혈관화 술식에서 다양한 상아질 접착제가 치아 변색과 칼슘 실리케이트 시멘트의 밀폐 효과에 미치는 영향에 대한 실험실적 연구

  • Jieun Han (Department of Pediatric Dentistry, School of Dentistry, Kyungpook National University) ;
  • Gimin Kim (Department of Pediatric Dentistry, School of Dentistry, Kyungpook National University) ;
  • Jaesik Lee (Department of Pediatric Dentistry, School of Dentistry, Kyungpook National University)
  • 한지은 (경북대학교 치과대학 소아치과학교실) ;
  • 김기민 (경북대학교 치과대학 소아치과학교실) ;
  • 이제식 (경북대학교 치과대학 소아치과학교실)
  • Received : 2023.05.07
  • Accepted : 2023.06.27
  • Published : 2023.08.31

Abstract

This study aimed to compare the effects of applying different types of dentin bonding agents (DBAs) on tooth and on the sealing ability of CSC in regenerative endodontic procedures (REPs). Specimens were made from 120 human first premolars and categorized into the following groups based on the presence and the type of DBA: Group I, no bonding; Group II, One-Step; Group III, Clearfil SE Bond; and Group IV, Single Bond Universal. Subsequently, the groups were categorized into Subgroup a and Subgroup b, in which Endocem MTA® Premixed Regular and Biodentine were used, respectively. The CIE L*a*b* on the photos taken prior to TAP placement (S0), 1 week after TAP placement (S1), 2 weeks after TAP placement (S2), 4 weeks after TAP placement (S3) and 2 weeks after CSC placement (S4) was used to assess discoloration. The samples were submerged in a 2% methylene blue solution and magnified by 30 times under a stereomicroscope to assess microleakage. The application of DBA during REP reduced crown discoloration, while Biodentine showed minimal discoloration with or without DBA and regardless of the DBA type. Additionally, the application of DBA did not increase microleakage.

본 연구는 치수 재혈관화 과정 중 상아질 접착제 도포 유무 및 종류가 치아 변색과 미세 누출에 미치는 영향을 평가하고자 하였다. 시편은 사람의 발거된 소구치 120개로 제작하였으며, 상아질 접착제 종류에 따라 다음과 같이 분류하였다. Group I. No bonding; Group II. One-Step; Group III. Clearfil SE Bond; Group IV. Single Bond Universal. 각 그룹은 칼슘 규산염계 시멘트인 Endocem MTA® Premixed Regular와 Biodentine의 사용에 따라 Subgroup a와 Subgroup b로 나뉘어 총 8개의 그룹으로 분류되었다. 치관변색 평가를 위해 초진(S0), 항생제 도포 1주(S1), 2주(S2), 4주 경과(S3)와 칼슘 규산염계 시멘트 적용 2주 경과(S4) 각 시점에서 사진을 촬영해 CIE L*a*b*를 측정하였다. 모든 시편은 2% 메틸렌 블루 용액에 침적 후 30배율로 관찰하여 미세누출을 평가하였다. 상아질 접착제 도포는 치관 변색을 감소시켰고, Biodentine은 상아질 접착제의 도포 유무 및 종류와 상관없이 최소한의 변색을 보였다. 또한 상아질 접착제 도포는 미세 누출을 증가시키지 않았다.

Keywords

References

  1. Iwaya SI, Ikawa M, Kubota M : Revascularization of an immature permanent tooth with apical periodontitis and sinus tract. Dent Traumatol, 17:185-187, 2001. https://doi.org/10.1034/j.1600-9657.2001.017004185.x
  2. American association of Endodontists : AAE clinical considerations for a regenerative procedure. Available from: https://www.aae.org/specialty/wp-content/uploads/sites/2/2017/06/currentregenerativeendodonticconsiderations.pdf (Accessed on August 8, 2023).
  3. Hoshino E, Kurihara-Ando N, Sato I, Uematsu H, Sato M, Kota K, Iwaku M : In-vitro antibacterial susceptibility of bacteria taken from infected root dentine to a mixture of ciprofloxacin, metronidazole and minocycline. Int Endod J, 29:125-130, 1996. https://doi.org/10.1111/j.1365-2591.1996.tb01173.x
  4. Banchs F, Trope M : Revascularization of immature permanent teeth with apical periodontitis: new treatment protocol? J Endod, 30:196-200, 2004. https://doi.org/10.1097/00004770-200404000-00003
  5. Kim JH, Kim Y, Shin SJ, Park JW, Jung IY : Tooth discoloration of immature permanent incisor associated with triple antibiotic therapy: a case report. J Endod, 36:1086-1091, 2010. https://doi.org/10.1016/j.joen.2010.03.031
  6. Kahler B, Rossi-Fedele G : A review of tooth discoloration after regenerative endodontic therapy. J Endod, 42:563-569, 2016. https://doi.org/10.1016/j.joen.2015.12.022
  7. Tanase S, Tsuchiya H, Yao J, Ohmoto S, Takagi N, Yoshida S : Reversed-phase ion-pair chromatographic analysis of tetracycline antibiotics: application to discolored teeth. J Chromatogr B Biomed Sci Appl, 706:279-285, 1998. https://doi.org/10.1016/S0378-4347(97)00563-X
  8. Kang SH, Shin YS, Lee HS, Kim SO, Shin Y, Jung IY, Song JS : Color changes of teeth after treatment with various mineral trioxide aggregate-based materials: an ex vivo study. J Endod, 41:737-741, 2015. https://doi.org/10.1016/j.joen.2015.01.019
  9. Felman D, Parashos P : Coronal tooth discoloration and white mineral trioxide aggregate. J Endod, 39:484-487, 2013. https://doi.org/10.1016/j.joen.2012.11.053
  10. Asgary S, Parirokh M, Eghbal MJ, Brink F : Chemical differences between white and gray mineral trioxide aggregate. J Endod, 31:101-103, 2005. https://doi.org/10.1097/01.DON.0000133156.85164.B2
  11. Galler KM, Krastl G, Simon S, Van Gorp G, Meschi N, Vahedi B, Lambrechts P : European Society of Endodontology position statement: revitalization procedures. Int Endod J, 49:717-723, 2016. https://doi.org/10.1111/iej.12629
  12. Sato I, Ando-Kurihara N, Kota K, Iwaku M, Hoshino E : Sterilization of infected root-canal dentine by topical application of a mixture of ciprofloxacin, metronidazole and minocycline in situ. Int Endod J, 29:118-124, 1996. https://doi.org/10.1111/j.1365-2591.1996.tb01172.x
  13. Kohli MR, Yamaguchi M, Setzer FC, Karabucak B : Spectrophotometric analysis of coronal tooth discoloration induced by various bioceramic cements and other endodontic materials. J Endod, 41:1862-1866, 2015. https://doi.org/10.1016/j.joen.2015.07.003
  14. Marconyak Jr LJ, Kirkpatrick TC, Roberts HW, Roberts MD, Aparicio A, Himel VT, Sabey KA : A comparison of coronal tooth discoloration elicited by various endodontic reparative materials. J Endod, 42:470-473, 2016. https://doi.org/10.1016/j.joen.2015.10.013
  15. Lenherr P, Allgayer N, Weiger R, Filippi A, Attin T, Krastl G : Tooth discoloration induced by endodontic materials: a laboratory study. Int Endod J, 45:942-949, 2012. https://doi.org/10.1111/j.1365-2591.2012.02053.x
  16. Abimanyu R, Meidyawati R : Differences in microleakage between MTA and Biodentine™ as material for treatment of access perforation. J Phys Conf Ser, 884:012104, 2017.
  17. Reyes-Carmona JF, Felippe MS, Felippe WT : The bio-mineralization ability of mineral trioxide aggregate and Portland cement on dentin enhances the pushout strength. J Endod, 36:286-291, 2010. https://doi.org/10.1016/j.joen.2009.10.009
  18. Shokouhinejad N, Khoshkhounejad M, Alikhasi M, Bagheri P, Camilleri J : Prevention of coronal discoloration induced by regenerative endodontic treatment in an ex vivo model. Clin Oral Investig, 22:1725-1731, 2018. https://doi.org/10.1007/s00784-017-2266-0
  19. Guler AU, Kurt S, Kulunk T : Effects of various finishing procedures on the staining of provisional restorative materials. J Prosthet Dent, 93:453-458, 2005. https://doi.org/10.1016/j.prosdent.2005.02.001
  20. Ruyter IE, Nilner K, Moller B : Color stability of dental composite resin materials for crown and bridge veneers. Dent Mater, 3:246-251, 1987. https://doi.org/10.1016/S0109-5641(87)80081-7
  21. Morresi AL, D'Amario M, Capogreco M, Gatto R, Marzo G, D'Arcangelo C, Monaco A : Thermal cycling for restorative materials: does a standardized protocol exist in laboratory testing? A literature review. J Mech Behav Biomed Mater, 29:295-308, 2014. https://doi.org/10.1016/j.jmbbm.2013.09.013
  22. Parsons JR, Walton RE, Ricks-Williamson L : In vitro longitudinal assessment of coronal discoloration from endodontic sealers. J Endod, 27:699-702, 2001. https://doi.org/10.1097/00004770-200111000-00012
  23. Partovi M, Al-Havvaz AH, Soleimani B : In vitro computer analysis of crown discolouration from commonly used endodontic sealers. Aust Endod J, 32:116-119, 2006. https://doi.org/10.1111/j.1747-4477.2006.00034.x
  24. Khim TP, Sanggar V, Shan TW, Peng KC, Western JS, Dicksit DD : Prevention of coronal discoloration induced by root canal sealer remnants using Dentin Bonding agent: An in vitro study. J Conserv Dent, 21:562-568, 2018. https://doi.org/10.4103/JCD.JCD_115_18
  25. Cal E, Guneri P, Kose T : Comparison of digital and spectrophotometric measurements of colour shade guides. J Oral Rehabil, 33:221-228, 2006. https://doi.org/10.1111/j.1365-2842.2005.01560.x
  26. Anand D, Kumar G, Anand DY, Sundar MK, Sharma R, Gaurav A : Shade selection: Spectrophotometer vs. digital camera - A comparative in vitro study. IP Ann Prosthodont Restor Dent, 2:73-78, 2016.
  27. Miyajiwala JS, Kheur MG, Patankar AH, Lakha TA : Comparison of photographic and conventional methods for tooth shade selection: A clinical evaluation. J Indian Prosthodont Soc, 17:273-281, 2017. https://doi.org/10.4103/jips.jips_342_16
  28. Shokouhinejad N, Khoshkhounejad M, Alikhasi M, Bagheri P, Camilleri J : Prevention of coronal discoloration induced by regenerative endodontic treatment in an ex vivo model. Clin Oral Investig, 22:1725-1731, 2018. https://doi.org/10.1007/s00784-017-2266-0
  29. Poly A, Marques F, Fidel SR, Monnerat AF, Sassone LM : Ability of two single-step restorative materials to avoid crown darkening caused by intracanal minocycline paste. Clin Oral Investig, 23:1281-1286, 2019. https://doi.org/10.1007/s00784-018-2557-0
  30. Breschi L, Mazzoni A, Ruggeri A, Cadenaro M, Di Lenarda R, Dorigo EDS : Dental adhesion review: aging and stability of the bonded interface. Dent Mater, 24:90-101, 2008. https://doi.org/10.1016/j.dental.2007.02.009
  31. Choi YL, Jang YE, Kim BS, Kim JW, Kim Y : Pre-application of dentin bonding agent prevents discoloration caused by mineral trioxide aggregate. BMC Oral Health, 20:163, 2020.
  32. de Jesus LS, Volpato CAM, Bortoluzzi EA, da Silveira Teixeira C, Rossetto HL, de Carvalho Panzeri Pires-de-Souza F, da Fonseca Roberti Garcia L : Tooth discoloration induced by the different phases of a calcium aluminate cement: One-year assessment. J Esthet Restor Dent, 33:999-1009, 2021. https://doi.org/10.1111/jerd.12739
  33. Mozynska J, Metlerski M, Lipski M, Nowicka A : Tooth discoloration induced by different calcium silicate-based cements: a systematic review of in vitro studies. J Endod, 43:1593-1601, 2017. https://doi.org/10.1016/j.joen.2017.04.002
  34. Yoldas SE, Bani M, Atabek D, Bodur H : Comparison of the potential discoloration effect of bioaggregate, biodentine, and white mineral trioxide aggregate on bovine teeth: in vitro research. J Endod, 42:1815-1818, 2016. https://doi.org/10.1016/j.joen.2016.08.020
  35. Mozayeni MA, Milani AS, Marvasti LA, Asgary S : Cytotoxicity of calcium enriched mixture cement compared with mineral trioxide aggregate and intermediate restorative material. Aust Endod J, 38:70-75, 2012. https://doi.org/10.1111/j.1747-4477.2010.00269.x
  36. Oliveira SS, Pugach MK, Hilton JF, Watanabe LG, Marshall SJ, Marshall Jr GW : The influence of the dentin smear layer on adhesion: a self-etching primer vs. a total-etch system. Dent Mater, 19:758-767, 2003. https://doi.org/10.1016/S0109-5641(03)00023-X
  37. Proenca JP, Polido M, Osorio E, Erhardt MCG, Aguilera FS, Garcia-Godoy F, Osorio R, Toledano M : Dentin regional bond strength of self-etch and total-etch adhesive systems. Dent Mater, 23:1542-1548, 2007. https://doi.org/10.1016/j.dental.2007.02.001
  38. Koshiro K, Inoue S, Tanaka T, Koase K, Fujita M, Hashimoto M, Sano H : In vivo degradation of resin-dentin bonds produced by a self-etch vs. a total-etch adhesive system. Eur J Oral Sci, 112:368-375, 2004. https://doi.org/10.1111/j.1600-0722.2004.00141.x
  39. Carrilho M, Geraldeli S, Tay F, De Goes M, Carvalho RM, Tjaderhane L, Reis A, Hebling J, Mazzoni A, Breschi L, Pashley D : In vivo preservation of the hybrid layer by chlorhexidine. J Dent Res, 86:529-533, 2007. https://doi.org/10.1177/154405910708600608
  40. Mazzoni A, Scaffa P, Carrilho M, Tjaderhane L, Di Lenarda R, Polimeni A, Tezvergil-Mutluay A, Tay FR, Pashley DH, Breschi L : Effects of etch-and-rinse and self-etch adhesives on dentin MMP-2 and MMP-9. J Dent Res, 92:82-86, 2013. https://doi.org/10.1177/0022034512467034
  41. Sanares AM, Itthagarun A, King NM, Tay FR, Pashley DH : Adverse surface interactions between one-bottle light-cured adhesives and chemical-cured composites. Dent Mater, 17:542-556, 2001. https://doi.org/10.1016/S0109-5641(01)00016-1
  42. Namazikhah MS, Nekoofar MH, Sheykhrezae MS, Salariyeh S, Hayes SJ, Bryant ST, Mohammadi MM, Dummer PMH : The effect of pH on surface hardness and microstructure of mineral trioxide aggregate. Int Endod J, 41:108-116, 2008. https://doi.org/10.1111/j.1365-2591.2007.01325.x
  43. Lee YL, Lee BS, Lin FH, Lin AY, Lan WH, Lin CP : Effects of physiological environments on the hydration behavior of mineral trioxide aggregate. Biomaterials, 25:787-793, 2004. https://doi.org/10.1016/S0142-9612(03)00591-X
  44. Saghiri MA, Lotfi M, Saghiri AM, Vosoughhosseini S, Fatemi A, Shiezadeh V, Ranjkesh B : Effect of pH on sealing ability of white mineral trioxide aggregate as a root-end filling material. J Endod, 34:1226-1229, 2008. https://doi.org/10.1016/j.joen.2008.07.017
  45. Torabinejad M, Hong CU, McDonald F, Pitt Ford TR : Physical and chemical properties of a new root-end filling material. J Endod, 21:349-353, 1995. https://doi.org/10.1016/S0099-2399(06)80967-2
  46. Watts JD, Holt DM, Beeson TJ, Kirkpatrick TC, Rutledge RE : Effects of pH and mixing agents on the temporal setting of tooth-colored and gray mineral trioxide aggregate. J Endod, 33:970-973, 2007. https://doi.org/10.1016/j.joen.2007.01.024
  47. Shokouhinejad N, Nekoofar MH, Iravani A, Kharrazifard MJ, Dummer PM : Effect of acidic environment on the push-out bond strength of mineral trioxide aggregate. J Endod, 36:871-874, 2010. https://doi.org/10.1016/j.joen.2009.12.025
  48. Hansen SR, Montgomery S : Effect of restoration thickness on the sealing ability of TERM. J Endod, 19:448-452, 1993. https://doi.org/10.1016/S0099-2399(06)80531-5
  49. Matloff IR, Jensen JR, Singer L, Tabibi A : A comparison of methods used in root canal sealability studies. Oral Surg Oral Med Oral Pathol, 53:203-208, 1982. https://doi.org/10.1016/0030-4220(82)90288-2
  50. Kersten HW, Moorer WR : Particles and molecules in endodontic leakage. Int Endod J, 22:118-124, 1989. https://doi.org/10.1111/j.1365-2591.1989.tb00909.x
  51. Camilleri J, Pitt Ford TR : Evaluation of the effect of tracer pH on the sealing ability of glass ionomer cement and mineral trioxide aggregate. J Mater Sci Mater Med, 19:2941-2948, 2008. https://doi.org/10.1007/s10856-008-3429-y
  52. Palma PJ, Marques JA, Antunes M, Falacho RI, Sequeira D, Roseiro L, Santos JM, Ramos JC : Effect of restorative timing on shear bond strength of composite resin/calcium silicate-based cements adhesive interfaces. Clin Oral Investig, 25:3131-3139, 2021. https://doi.org/10.1007/s00784-020-03640-7
  53. Ramos JC, Palma PJ, Nascimento R, Caramelo F, Messias A, Vinagre A, Santos JM : 1-year in vitro evaluation of tooth discoloration induced by 2 calcium silicate-based cements. J Endod, 42:1403-1407, 2016. https://doi.org/10.1016/j.joen.2016.06.012
  54. Marin PD, Heithersay GS, Bridges TE : A quantitative comparison of traditional and non-peroxide bleaching agents. Endod Dent Traumatol, 14:64-67, 1998. https://doi.org/10.1111/j.1600-9657.1998.tb00811.x
  55. Ausiello P, Cassese A, Miele C, Beguinot F, GarciaGodoy F, Di Jeso B, Ulianich L : Cytotoxicity of dental resin composites: an in vitro evaluation. J Appl Toxicol, 33:451-457, 2013. https://doi.org/10.1002/jat.1765