Annals of Laboratory Medicine

Korean Society for Laboratory Medicine (대한진단검사의학회)
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Virulence-associated Genome Sequences of Pasteurella canis and Unique Toxin Gene Prevalence of P. canis and Pasteurella multocida Isolated from Humans and Companion Animals

  • Haruno Yoshida (Laboratory of Infectious Diseases, Graduate School of Infection Control Sciences & Omura Satoshi Memorial Institute, Kitasato University) ;
  • Jung-Min Kim (Department of Laboratory Medicine, Kangdong Sacred Heart Hospital, Hallym University College of Medicine) ;
  • Takahiro Maeda (Laboratory of Infectious Diseases, Graduate School of Infection Control Sciences & Omura Satoshi Memorial Institute, Kitasato University) ;
  • Mieko Goto (Laboratory of Infectious Diseases, Graduate School of Infection Control Sciences & Omura Satoshi Memorial Institute, Kitasato University) ;
  • Yuzo Tsuyuki (Laboratory of Infectious Diseases, Graduate School of Infection Control Sciences & Omura Satoshi Memorial Institute, Kitasato University) ;
  • Sachiko Shibata (Division of Clinical Laboratory, Sanritsu Laboratory) ;
  • Kenichi Shizuno (Laboratory of Infectious Diseases, Graduate School of Infection Control Sciences & Omura Satoshi Memorial Institute, Kitasato University) ;
  • Katsuko Okuzumi (Laboratory of Infectious Diseases, Graduate School of Infection Control Sciences & Omura Satoshi Memorial Institute, Kitasato University) ;
  • Jae-Seok Kim (Department of Laboratory Medicine, Kangdong Sacred Heart Hospital, Hallym University College of Medicine) ;
  • Takashi Takahashi (Laboratory of Infectious Diseases, Graduate School of Infection Control Sciences & Omura Satoshi Memorial Institute, Kitasato University)
  • Received : 2022.09.07
  • Accepted : 2022.11.18
  • Published : 2023.05.01
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Abstract

Background: Comparative analysis of virulence factors (VFs) between Pasteurella canis and Pasteurella multocida are lacking, although both cause zoonotic infections. We determined the virulence-associated genome sequence characteristics of P. canis and assessed the toxin gene prevalence unique to P. canis among clinical isolates of P. canis and P. multocida. Methods: We selected 10 P. canis and 16 P. multocida whole-genome sequences (WGSs) from the National Center for Biotechnology database. The VFanalyzer tool was used to estimate P. canis-characteristic VFs. Amino acid sequences of VFs were compared with multiple-aligned sequences. The genome structure containing P. canis-characteristic and adjacent loci was compared to the corresponding P. multocida genome structure. After designing primer sequences and assessing their accuracy, we examined the gene prevalence of the P. canis-characteristic VFs using PCR among clinical isolates of P. multocida and P. canis. Results: Using VFanalyzer, we found virulence-associated cytolethal distending toxin (cdt) A-cdtB-cdtC loci common to all P. canis WGSs that were not found in P. multocida WGSs. Similarities in the multiple alignments of CdtA-CdtB-CdtC amino acid sequences were found among the 10 P. canis WGSs. Shared or similar loci around cdtA-cdtB-cdtC were identified between the P. canis and P. multocida genome structures. The PCR-based cdtA-cdtB-cdtC prevalence differed for P. canis and P. multocida clinical isolates. Conclusions: P. canis-specific cdtA-cdtB-cdtC prevalence was identified among clinical isolates. These three loci may be unique toxin genes and promising targets for the rapid identification of P. canis in clinical settings.

Keywords

Acknowledgement

We thank Goro Kurita, D.M.V. (Laboratory of Infectious Diseases, Graduate School of Infection Control Sciences, Kitasato University, Tokyo, Japan), Prof. Noriyuki Nagano (Department of Medical Sciences, Graduate School of Medicine, Science and Technology, Shinshu University, Nagano, Japan), and Mr. Tomohiro Fujita (Department of Clinical Laboratory, Kitasato University Medical Center, Kitasato University, Saitama, Japan) for their assistance.

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