Journal of Korean Neurosurgical Society

  • 제66권5호
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  • Pages.511-524
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  • 2023
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  • 2005-3711(pISSN)
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  • 1598-7876(eISSN)
대한신경외과학회 (The Korean Neurosurgical Society)
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Reparative, Neuroprotective and Anti-neurodegenerative Effects of Granulocyte Colony Stimulating Factor in Radiation-Induced Brain Injury Model

  • Gokhan Gurkan (Department of Neurosurgery, Izmir Katip Celebi University Ataturk Training and Research Hospital) ;
  • Ozum Atasoy (Department of Radiation Oncology, Kartal City Hospital) ;
  • Nilsu Cini (Department of Radiation Oncology, Kartal City Hospital) ;
  • Ibrahim Halil Sever (Department of Radiology, Demiroglu Bilim University) ;
  • Bahattin Ozkul (Department of Radiology, Istanbul Atlas University) ;
  • Gokhan Yaprak (Department of Radiation Oncology, Lutfi Kirdar Kartal Education and Research Hospital) ;
  • Cansin Sirin (Department of Histology and Embryology, Faculty of Medicine, Ege University) ;
  • Yigit Uyanikgil (Department of Histology and Embryology, Faculty of Medicine, Ege University) ;
  • Ceren Kizmazoglu (Department of Neurosurgery, Faculty of Medicine, Dokuz Eylul University) ;
  • Mumin Alper Erdogan (Department of Physiology, Katip Celebi University) ;
  • Oytun Erbas (Department of Physiology, Demiroglu Bilim University)
  • 투고 : 2023.03.07
  • 심사 : 2023.04.24
  • 발행 : 2023.09.01
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초록

Objective : This animal model aimed to compare the rat group that received brain irradiation and did not receive additional treatment (only saline) and the rat group that underwent brain irradiation and received Granulocyte colony stimulating factor (G-CSF) treatment. In addition, the effects of G-CSF on brain functions were examined by magnetic resonance (MR) imaging and histopathologically. Methods : This study used 24 female Wistar albino rats. Drug administration (saline or G-CSF) was started at the beginning of the study and continued for 15 days after whole-brain radiotherapy (WBRT). WBRT was given on day 7 of the start of the study. At the end of 15 days, the behavioral tests, including the three-chamber sociability test, open field test, and passive avoidance learning test, were done. After the behavioral test, the animals performed the MR spectroscopy procedure. At the end of the study, cervical dislocation was applied to all animals. Results : G-CSF treatment positively affected the results of the three-chamber sociability test, open-space test and passive avoidance learning test, cornu Ammonis (CA) 1, CA3, and Purkinje neuron counts, and the brain levels of brain-derived neurotrophic factor and postsynaptic density protein-95. However, G-CSF treatment reduced the glial fibrillary acidic protein immunostaining index and brain levels of malondialdehyde, tumor necrosis factor-alpha, nuclear factor kappa-B, and lactate. In addition, on MR spectroscopy, G-CSF had a reversible effect on brain lactate levels. Conclusion : In this first designed brain irradiation animal model, which evaluated G-CSF effects, we observed that G-CSF had reparative, neuroprotective and anti-neurodegenerative effects and had increased neurotrophic factor expression, neuronal counts, and morphology changes. In addition, G-CSF had a proven lactate-lowering effect in MR spectroscopy and brain materials.

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