Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
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Phytoceramide Alleviates the Carrageenan/Kaolin-Induced Arthritic Symptoms by Modulation of Inflammation

  • Bongjun Sur (Department of Molecular Medicine, School of Medicine, Ewha Womans University) ;
  • Mijin Kim (Department of Molecular Medicine, School of Medicine, Ewha Womans University) ;
  • Thea Villa (Department of Molecular Medicine, School of Medicine, Ewha Womans University) ;
  • Seikwan Oh (Department of Molecular Medicine, School of Medicine, Ewha Womans University)
  • Received : 2023.02.15
  • Accepted : 2023.05.30
  • Published : 2023.09.01
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Abstract

Phytoceramide (Pcer) is found mainly in plants and yeast. It can be neuroprotective and immunostimulatory on various cell types. In this study, the therapeutic effect of Pcer was explored using the carrageenan/kaolin (C/K)-induced arthritis rat model and fibroblast-like synoviocytes (FLS). Pcer treatment (1, 10, and 30 mg/kg/day) were given to the arthritic rats for 6 days after disease induction. Weight distribution ration (WDR), knee thickness, squeaking score, serum levels of proinflammatory mediators, and histological analysis were measured and performed to evaluate arthritic symptoms in the rat model. In interleukin (IL)-1β-stimulated FLS, proinflammatory mediators were measured after Pcer (1-30 µM) treatment. Arthritic symptoms in rats with Pcer treatment were significantly decreased at days 4 to 6 after C/K arthritis induction. Inflammation in the knee joints were also significantly decreased in rats with Pcer treatment. Furthermore, in IL-1β-stimulated FLS, the expressions of proinflammatory mediators were also inhibited by Pcer. As shown by the results, Pcer has anti-arthritic effects in the C/K rat model and in synovial cells, suggesting that Pcer has the potential to be a useful agent in arthritis treatment.

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References

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