Journal of Periodontal and Implant Science

  • 제53권4호
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  • Pages.248-258
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  • 2023
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  • 2093-2278(pISSN)
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  • 2093-2286(eISSN)
대한치주과학회 (Korean Academy of Periodontology)
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Temporal changes of periodontal tissue pathology in a periodontitis animal model

  • Hyunpil Yoon (Department of Periodontology and Research Institute of Oral Sciences, Gangneung-Wonju National University College of Dentistry) ;
  • Bo Hyun Jung (Department of Anatomy and Research Institute of Oral Sciences, Gangneung-Wonju National University College of Dentistry) ;
  • Ki-Yeon Yoo (Department of Anatomy and Research Institute of Oral Sciences, Gangneung-Wonju National University College of Dentistry) ;
  • Jong-Bin Lee (Department of Periodontology and Research Institute of Oral Sciences, Gangneung-Wonju National University College of Dentistry) ;
  • Heung-Sik Um (Department of Periodontology and Research Institute of Oral Sciences, Gangneung-Wonju National University College of Dentistry) ;
  • Beom-Seok Chang (Department of Periodontology and Research Institute of Oral Sciences, Gangneung-Wonju National University College of Dentistry) ;
  • Jae-Kwan Lee (Department of Periodontology and Research Institute of Oral Sciences, Gangneung-Wonju National University College of Dentistry)
  • 투고 : 2022.08.01
  • 심사 : 2022.10.19
  • 발행 : 2023.08.31
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초록

Purpose: This study aimed to characterize the early stages of periodontal disease and determine the optimal period for its evaluation in a mouse model. The association between the duration of ligation and its effect on the dentogingival area in mice was evaluated using micro-computed tomography (CT) and histological analysis. Methods: Ninety mice were allocated to an untreated control group or a ligation group in which periodontitis was induced by a 6-0 silk ligation around the left second maxillary molar. Mice were sacrificed at 1, 2, 3, 4, 5, 8, 11, and 14 days after ligature placement. Alveolar bone destruction was evaluated using micro-CT. Histological analysis was performed to assess the immune-inflammatory processes in the periodontal tissue. Results: No significant difference in alveolar bone loss was found compared to the control group until day 3 after ligature placement, and a gradual increase in alveolar bone loss was observed from 4 to 8 days following ligature placement. No significant between-group differences were observed after 8 days. The histological analysis demonstrated that the inflammatory response was evident from day 4. Conclusions: Our findings in a mouse model provide experimental evidence that ligature-induced periodontitis models offer a consistent progression of disease with marginal attachment down-growth, inflammatory infiltration, and alveolar bone loss.

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과제정보

This study was supported by the 2021 Cooperative Research Program (grant number CR2101) of Gangneung-Wonju National University Dental Hospital.

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