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Effect of Solid-State Fermented Brown Rice Extracts on 3T3-L1 Adipocyte Differentiation

  • Su Bin Ji (Department of Food Science and Biotechnology, College of Engineering, Global K-Food Research Center, Hankyong National University) ;
  • Chae Hun Ra (Department of Food Science and Biotechnology, College of Engineering, Global K-Food Research Center, Hankyong National University)
  • Received : 2023.01.30
  • Accepted : 2023.04.05
  • Published : 2023.07.28

Abstract

Aspergillus oryzae KCCM 11372 was used to enhance the production of β-glucan using humidity control strategies. Under conditions of 60% humidity, solid-state fermentation (SSF) increased the yields of enzymes (amylase and protease), fungal biomass (ergosterol), and β-glucan. The maximum concentrations obtained were 14800.58 U/g at 72 h, 1068.14 U/g at 120 h, 1.42 mg/g at 72 h, and 12.0% (w/w) at 72 h, respectively. Moreover, the β-glucan containing fermented brown rice (β-glucan-FBR) extracts at concentrations of 25-300 ㎍/ml was considered noncytotoxic to 3T3-L1 preadipocytes. We then studied the inhibitory effects of the extracts on fat droplet formation in 3T3-L1 cells. As a result, 300 ㎍/ml of β-glucan-FBR extracts showed a high inhibition of 38.88% in lipid accumulation. Further, these extracts inhibited adipogenesis in the 3T3-L1 adipocytes by decreasing the expression of C/EBPα, PPARγ, aP2, and GLUT4 genes.

Keywords

Acknowledgement

This work was supported by the National Research Foundation of Korea(NRF) grant funded by the Korea government (MSIT) (No. 2022R1F1A1074594).

References

  1. Singhania RR, Sukumaran RK, Patel AK, Larroche C, Pandey A. 2010. Advancement and comparative profiles in the production technologies using solid-state and submerged fermentation for microbial cellulases. Enzyme Microb. Technol. 46: 541-549. https://doi.org/10.1016/j.enzmictec.2010.03.010
  2. Sadh PK, Kumar S, Chawla P, Duhan JS. 2018. Fermentation: a boon for production of bioactive compounds by processing of food industries wastes (by-products). Molecules 23: 2560.
  3. Jung TD, Shin GH, Kim JM, Choi SI, Lee JH, Lee SJ, et al. 2017. Comparative analysis of γ-oryzanol, β-glucan, total phenolic content and antioxidant activity in fermented rice bran of different varieties. Nutrients 9: 571-582. https://doi.org/10.3390/nu9060571
  4. Ji SB, Ra CH. 2021. Coproduction of enzymes and beta-glucan by Aspergillus oryzae using solid-state fermentation of brown rice. J. Microbiol. Biotechnol. 31: 1028-1034. https://doi.org/10.4014/jmb.2105.05005
  5. Alminger M, Eklund-Jonsson C. 2008. Whole-grain cereal products based on a high-fibre barley or oat genotype lower post-prandial glucose and insulin responses in healthy humans. Eur. J. Nutr. 47: 294-300. https://doi.org/10.1007/s00394-008-0724-9
  6. Nicolosi R, Bell SJ, Bistrian BR, Greenberg I, Forse RA, Blackburn GL. 1999. Plasma lipid changes after supplementation with β-glucan fiber from yeast. AMJ. Clin. Nutr. 70: 208-212. https://doi.org/10.1093/ajcn.70.2.208
  7. Artiss JD, Brogan K, Brucal M, Moghaddam M, Jen KLC. 2006. The effects of a new soluble dietary fiber on weight gain and selected blood parameters in rats. Metabolism 55: 195-202. https://doi.org/10.1016/j.metabol.2005.08.012
  8. Kanagasabapathy G, Chua KH, Malek SNA, Vikineswary S, Kuppusamy UR. 2014. AMP-activated protein kinase mediates insulin-like and lipo-mobilising effects of β-glucan-rich polysaccharides isolated from Pleurotus sajor-caju (Fr.), Singer mushroom, in 3T3-L1 cells. Food Chem. 145: 198-204. https://doi.org/10.1016/j.foodchem.2013.08.051
  9. Cani PD, Bibiloni R, Knauf C, Waget A, Neyrinck AM, Delzenne NM, et al. 2008. Changes in gut microbiota control metabolic endotoxemia-induced inflammation in high-fat diet-induced obesity and diabetes in mice. Diabetes 57: 1470-1481. https://doi.org/10.2337/db07-1403
  10. Green H, Kehinde O. 1975. An established preadipose cell line and its differentiation in culture. II. Factors affecting the adipose conversion. Cell 5: 19-27. https://doi.org/10.1016/0092-8674(75)90087-2
  11. Ahmad B, Serpell CJ, Fong IL, Wong EH. 2020. Molecular mechanisms of adipogenesis: the anti-adipogenic role of AMP-activated protein kinase. Front. Mol. Biosci. 7: 76.
  12. Kopecky J, Clarke G, Enerback S, Spiegelman B, Kozak LP. 1995. Expression of the mitochondrial uncoupling protein gene from the aP2 gene promoter prevents genetic obesity. J. Clin. Invest. 96: 2914-2923. https://doi.org/10.1172/JCI118363
  13. Govers R. 2014. Molecular mechanisms of GLUT4 regulation in adipocytes. Diabetes Metab. J. 40: 400-410. https://doi.org/10.1016/j.diabet.2014.01.005
  14. Yoo HU, Ko MJ, Chung MS. 2020. Hydrolysis of beta-glucan in oat flour during subcritical-water extraction. Food Chem. 308: 125670.
  15. Lim SM, Goh YM, Kuan WB, Loh SP. 2014. Effect of germinated brown rice extracts on pancreatic lipase, adipogenesis and lipolysis in 3T3-L1 adipocytes. Lipids Health. Dis. 13: 169.
  16. Ye C, Zhang R, Dong L, Chi J, Huang F, Dong L, et al. 2022. α-Glucosidase inhibitors from brown rice bound phenolics extracts (BRBPE): identification and mechanism. Food Chem. 372: 131306.
  17. Fajriah S, Sinurat E, Megawati M, Darmawan A, Meilawati L, Handayani S, et al. 2018. Identification of β-glucan and α-glucosidase inhibitory activity from Seagrape Caulerpa lentillifera extracts. AIP Conf. Proc. 2024: 020026.
  18. Vaidya H, Goyal RK, Cheema SK. 2013. Anti-diabetic activity of swertiamarin is due to an active metabolite, gentianine, that upregulates PPARγ gene expression in 3T3-L1 cells. Phytother. Res. 27: 624-627. https://doi.org/10.1002/ptr.4763
  19. Ramirez-Zacarias JL, Castro-Munozledo F, Kuri-Harcuch W. 1992. Quantitation of adipose conversion and triglycerides by staining intracytoplasmic lipids with oil red O. Histochem. 97: 493-497. https://doi.org/10.1007/BF00316069
  20. Kowalska K, Olejnik A, Rychlik J, Grajek W. 2014. Cranberries (Oxycoccus quadripetalus) inhibit adipogenesis and lipogenesis in 3T3-L1 cells. Food Chem. 148: 246-252. https://doi.org/10.1016/j.foodchem.2013.10.032
  21. He Q, Chen HZ. 2013. Pilot-scale gas double-dynamic solid-state fermentation for the production of industrial enzymes. Food Bioprocess Technol. 6: 2916-2924. https://doi.org/10.1007/s11947-012-0956-9
  22. Reynaldo DCQ, Sevastianos R, Daniel H, Raul R, Francisco C, Cristobal NA. 2015. Challenges and opportunities of the biopesticides production by solid-state fermentation: filamentous fungi as a model. Crit. Rev. Biotechnol. 35: 326-333. https://doi.org/10.3109/07388551.2013.857292
  23. Han JA, Lim ST. 2009. Effect of presoaking on textural, thermal, and digestive properties of cooked brown rice. Cereal Chem. 86: 100-105. https://doi.org/10.1094/CCHEM-86-1-0100
  24. Mantovani MS, Bellini MF, Angeli JPF, Oliveira RJ, Silva AF, Ribeiro LR. 2008. β-glucans in promoting health: prevention against mutation and cancer. Mutat. Res. 658: 154-161. https://doi.org/10.1016/j.mrrev.2007.07.002
  25. Ji Y, Liu D, Zhao J, Zhao J, Li H, Li L. 2021. In vitro and in vivo inhibitory effect of anthocyanin-rich bilberry extract on α-glucosidase and α-amylase. LWT-Food Sci. Technol. 145: 111484.
  26. ISO 10993-5:2009 Biological evaluation of medical devices. Part 5: Tests for in vitro cytotoxicity; International Organization for Standardization: Geneva, Switzerland
  27. Jang SJ, Xu Z. 2009. Lipophilic and hydrophilic antioxidants and their antioxidant activities in purple rice bran. J. Agric. Food Chem. 57: 858-862. https://doi.org/10.1021/jf803113c
  28. Verardo V, Gomez-Caravaca AM, Marconi E, Segura-Carretero A, Garrido-Frenich A, Fernandez-Gutierrez A. 2016. Determination of lipophilic and hydrophilic bioactive compounds in raw and parboiled rice bran. RSC Adv. 6: 50786-50796. https://doi.org/10.1039/C6RA04836F
  29. Zuo, Y, Qiang L, Farmer SR. 2006. Activation of CCAAT/enhancer-binding protein (C/EBP) α expression by C/EBPβ during adipogenesis requires a peroxisome proliferator-activated receptor-γ-associated repression of HDAC1 at the C/EBPα gene promoter. J. Biol. Chem. 281: 7960-7967. https://doi.org/10.1074/jbc.M510682200
  30. Zhu, Y, Yao Y, Gao Y, Hu Y, Shi Z, Ren G. 2016. Suppressive effects of barley β-glucans with different molecular weight on 3T3-L1 adipocyte differentiation. J. Food Sci. 81: H786-H793. https://doi.org/10.1111/1750-3841.13226
  31. Rosen ED, Walkey CJ, Puigserver P, Spiegelman BM. 2000. Transcriptional regulation of adipogenesis. Gene Dev. 14: 1293-1307. https://doi.org/10.1101/gad.14.11.1293
  32. Shepherd PR, Gnudi L, Tozzo E, Yang H, Leach F, Kahn BB. 1993. Adipose cell hyperplasia and enhanced glucose disposal in transgenic mice overexpressing GLUT4 selectively in adipose tissue. J. Biol. Chem. 268: 22243-22246. https://doi.org/10.1016/S0021-9258(18)41516-5
  33. Kim MJ, Kim OH, Cheong C, Jang KH, Kim CH, Kang SA. 2015. β-glucan from Aureobasidium species inhibits fat accumulation in 3T3-L1 adipocyte differentiation. Food Sci. Biotechnol. 24: 1147-1150. https://doi.org/10.1007/s10068-015-0146-4
  34. Henderson, AJ, Ollila CA, Kumar A, Borresen EC, Raina K, Agarwal R, et al. 2012. Chemopreventive properties of dietary rice bran: current status and future prospects. Adv. Nutr. 3: 643-653. https://doi.org/10.3945/an.112.002303