Fisheries and Aquatic Sciences

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Genetic characterization of alloherpesvirus (cyprinid herpesvirus-2 and koi herpesvirus) and poxvirus (carp edema virus) identified from domestic and imported cyprinids in Korea

  • Ye Jin Jeong (Department of Aquatic Life Medicine, Pukyong National University) ;
  • Yu Gyeong Jeon (Department of Aquatic Life Medicine, Pukyong National University) ;
  • Hee Ju Choi (Department of Aquatic Life Medicine, Pukyong National University) ;
  • Eun Jin Baek (Department of Aquatic Life Medicine, Pukyong National University) ;
  • Guk Hyun Kim (Department of Aquatic Life Medicine, Pukyong National University) ;
  • Yun Jung Yang (Department of Aquatic Life Medicine, Pukyong National University) ;
  • Min Jae Kim (Department of Aquatic Life Medicine, Pukyong National University) ;
  • Joon Gyu Min (Department of Aquatic Life Medicine, Pukyong National University) ;
  • Kwang Il Kim (Department of Aquatic Life Medicine, Pukyong National University)
  • Received : 2023.01.30
  • Accepted : 2023.05.11
  • Published : 2023.07.31
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Abstract

Cyprinids are popular species for aquaculture worldwide, with Asia being a significant contributor to their production. In Korea, common carp (Cyprinus carpio), koi carp (Cyprinus rubrofuscus), and goldfish (Carassius auratus) are cultivated domestically and imported for ornamental or human consumption purposes. Among the viruses that infect cyprinids, cyprinid herpesvirus-2 (CyHV-2), koi herpesvirus (KHV, also known as cyprinid herpesvirus-3), and carp edema virus (CEV) are of particular concern as they cause substantial economic losses to the aquaculture industry. In this study, we investigated these viruses in both of domestic and imported cyprinids. Our results revealed that CyHV-2 was only detected in imported goldfish from Thailand. To further investigate the genetic characteristics of them, the marker A region was analyzed. Despite belonging to the same cluster with isolates from China, France, Poland, and Israel, CyHV-2 detected in this study showed distinct differences in their repetitive sequence sizes. Furthermore, two different forms of KHV/CEV coinfection were identified from domestic koi carp, both of which exhibited typical symptoms. Phylogenetic analysis showed that one KHV isolate (ScKc-2105-K) was of the Asian type and closely related to isolates from Japan, Indonesia, Belgium, Taiwan, and China. Two CEV isolates (ScKc-2105-CE and GhKc-2207-CE) be- longed to the IIa type and showed high similarity with isolates from the USA, France, and Korea. Notably, koi carp injected with cultured KHV (ScKc-2105-K) showed 78.0% cumulative mortality within 14 days post-injection (dpi). Our findings support the importance of regular surveillance of viral diseases in cyprinids.

Keywords

Acknowledgement

This research was supported by the Korea Institute of Marine Science & Technology Promotion (KIMST) and funded by the Ministry of Oceans and Fisheries, Korea (201903922).

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