Effect of Brussels Sprouts Extract on Inflammatory Cytokine Inhibition

방울양배추 추출물의 염증성 사이토카인 억제에 미치는 영향

This paper was conducted to examine the effect of Brussels Sprouts Extract on the inhibition of pro-inflammatory cytokines. The inflammatory response is manifested by mediators such as reactive oxygen species and inflammatory cytokines such as TNF-α, IL-1β, and IL-8. Therefore, this paper examined the toxicity to cells using the MTS assay, stimulated RAW264.7 macrophages with lipopolysaccharide (LPS), and stimulated reactive oxygen species such as NO and TNF-α, IL-1β, and IL-8. Inhibition of inflammatory cytokines after treatment with 10 mg/mL, 100 mg/mL, and 1000 mg/mL of Brussels Sprouts Extract was investigated. As a result of the experiment, Brussels Sprouts Extract inhibited NO production, TNF-α and IL-8 in a concentration-dependent manner without cytotoxicity, and showed significant inhibition especially at a concentration of 1000 mg/mL. Brussels Sprouts Extract, which inhibits the production of inflammatory cytokines, suggests the possibility of reducing inflammatory response and controlling inflammation, and can be seen as providing potential as a health functional food or prevention and treatment of inflammation.

본 논문은 Brussels Sprouts Extract의 염증성사이토카인의 억제에 미치는 영향을 살펴보기 위하여 진행되었다. 염증반응은 활성산소와 같은 매개인자와 염증성 사이토카인인 TNF-α나 IL-1β, IL-8에 의해 나타난다. 이에 본 논문은 MTS assay를 이용하여 세포에 대한 생존율을 살펴보고, RAW264.7 대식세포에 lipopolysaccharide (LPS) 자극에 의해 생성된 NO와 TNF-α나 IL-1β, IL-8과 같은 염증성 사이토카인에 대한 억제를 살펴보았다. Brussels Sprouts Extract 10mg/mL, 100mg/mL, 1000mg/mL 농도로 처리 후 억제 정도를 살펴본 결과, Brussels Sprouts Extract은 세포 독성 없이 NO생성과 TNF-α, IL-8을 농도 의존적으로 억제하였으며, 특히 1000mg/mL 농도에서는 유의한 억제를 보였다. 염증성 사이토카인의 생성을 저해한 Brussels Sprouts Extract은 염증반응 감소와 염증조절의 가능성을 시사하고 건강기능성 식품이나 염증 예방 및 치료제로서 잠재력을 제공한다고 볼 수 있다.