Animal Bioscience

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Change in intestinal alkaline phosphatase activity is a hallmark of antibiotic-induced intestinal dysbiosis

  • Wijesooriya Mudhiyanselage Nadeema Dissanayake (Department of Agricultural Education, College of Education, Sunchon National University) ;
  • Malavige Romesha Chandanee (Department of Agricultural Education, College of Education, Sunchon National University) ;
  • Sang-Myeong Lee (Laboratory of Veterinary Virology, College of Veterinary Medicine, Chungbuk National University) ;
  • Jung Min Heo (College of Agriculture and Life Sciences, Department of Animal Science and Biotechnology, Chungnam National University) ;
  • Young-Joo Yi (Department of Agricultural Education, College of Education, Sunchon National University)
  • Received : 2023.02.15
  • Accepted : 2023.04.07
  • Published : 2023.09.01
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Abstract

Objective: Intestinal alkaline phosphatase (IAP) maintains intestinal homeostasis by detoxifying bacterial endotoxins and regulating gut microbiota, and lipid absorption. Antibiotics administered to animals can cause gut dysbiosis and barrier disruption affecting animal health. Therefore, the present study sought to investigate the role of IAP in the intestinal environment in dysbiosis. Methods: Young male mice aged 9 weeks were administered a high dose of antibiotics to induce dysbiosis. They were then sacrificed after 4 weeks to collect the serum and intestinal organs. The IAP activity in the ileum and the level of cytokines in the serum samples were measured. Quantitative real-time polymerase chain reaction analysis of RNA from the intestinal samples was performed using primers for tight junction proteins (TJPs) and proinflammatory cytokines. The relative intensity of IAP and toll-like receptor 4 (TLR4) in intestinal samples was evaluated by western blotting. Results: The IAP activity was significantly lower in the ileum samples of the dysbiosis-induced group compared to the control. The interleukin-1 beta, interleukin-6, and tumor necrosis factor-alpha concentrations were significantly higher in the ileum samples of the dysbiosis-induced group. The RNA expression levels of TJP2, claudin-3, and claudin-11 showed significantly lower values in the intestinal samples from the dysbiosis-induced mice. Results from western blotting revealed that the intensity of IAP expression was significantly lower in the ileum samples of the dysbiosis-induced group, while the intensity of TLR4 expression was significantly higher compared to that of the control group without dysbiosis. Conclusion: The IAP activity and relative mRNA expression of the TJPs decreased, while the levels of proinflammatory cytokines increased, which can affect intestinal integrity and the function of the intestinal epithelial cells. This suggests that IAP is involved in mediating the intestinal environment in dysbiosis induced by antibiotics and is an enzyme that can potentially be used to maintain the intestinal environment in animal health care.

Keywords

Acknowledgement

This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korean government (MSIT) (NRF-2020R1A2C1014007 and RS-2023-00245276).

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