Nutrition Research and Practice

The Korean Nutrition Society (한국영양학회)
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Protective effects of red orange (Citrus sinensis [L.] Osbeck [Rutaceae]) extract against UVA-B radiation-induced photoaging in Skh:HR-2 mice

  • Yoon Hee Kim (Technology Development Center, BTC Corporation) ;
  • Cho Young Lim (Technology Development Center, BTC Corporation) ;
  • Jae In Jung (Industry Coupled Cooperation Center for Bio Healthcare Materials, Hallym University) ;
  • Tae Young Kim (Technology Development Center, BTC Corporation) ;
  • Eun Ji Kim (Industry Coupled Cooperation Center for Bio Healthcare Materials, Hallym University)
  • Received : 2022.11.15
  • Accepted : 2023.01.30
  • Published : 2023.08.01
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Abstract

BACKGROUND/OBJECTIVES: The skin is the outermost organ of the human body and plays a protective role against external environmental damages, such as sunlight and pollution, which affect anti-oxidant defenses and skin inflammation, resulting in erythema or skin reddening, immunosuppression, and epidermal DNA damage. MATERIALS/METHODS: The present study aimed to investigate the potential protective effects of red orange complex H extract (ROC) against ultraviolet (UV)-induced skin photoaging in Skh:HR-2 mice. ROC was orally administered at doses of 20, 40, and 80 mg/kg/day for 13 weeks, along with UV irradiation of the mice for 10 weeks. RESULTS: ROC improved UV-induced skin barrier parameters, including erythema, melanin production, transepidermal water loss, elasticity, and wrinkle formation. Notably, ROC inhibited the mRNA expression of pro-inflammatory cytokines (interleukin 6 and tumor necrosis factor α) and melanogenesis. In addition, ROC recovered the UV-induced decrease in the hyaluronic acid and collagen levels by enhancing genes expression. Furthermore, ROC significantly downregulated the protein and mRNA expression of matrix metalloproteinases responsible for collagen degradation. These protective effects of ROC against photoaging are associated with the suppression of UV-induced phosphorylation of c-Jun NH2-terminal kinase and activator protein 1 activation. CONCLUSIONS: Altogether, our findings suggest that the oral administration of ROC exerts potential protective activities against photoaging in UV-irradiated hairless mice.

Keywords

Acknowledgement

The authors would like to express their gratitude to Bionap S.r.l for providing the red orange standardized extract.

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