방사선산업학회지 (Journal of Radiation Industry)

한국방사선산업학회 (Korean Society of Radiation Industry)
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생물정화를 위한 세슘 및 방사선 저항성 세균의 분리

Isolation of Cesium and Radiation Resistance Bacteria for Bioremediation

  • 김재훈 (한국원자력연구원 첨단방사선연구소) ;
  • 류재혁 (한국원자력연구원 첨단방사선연구소) ;
  • 김상훈 (한국원자력연구원 첨단방사선연구소) ;
  • 안준우 (한국원자력연구원 첨단방사선연구소) ;
  • 권순재 (한국원자력연구원 첨단방사선연구소) ;
  • 김진백 (한국원자력연구원 첨단방사선연구소) ;
  • 김민규 (한국원자력연구원 첨단방사선연구소) ;
  • 임상용 (한국원자력연구원 첨단방사선연구소) ;
  • 박재남 (송원대학교 식품영양학과)
  • Jae Hoon Kim (Korea Atomic Energy Research Institute (KAERI)) ;
  • Jai Hyunk Ryu (Korea Atomic Energy Research Institute (KAERI)) ;
  • Sang Hoon Kim (Korea Atomic Energy Research Institute (KAERI)) ;
  • Joon Woo Ahn (Korea Atomic Energy Research Institute (KAERI)) ;
  • Soon Jae Kwon (Korea Atomic Energy Research Institute (KAERI)) ;
  • Jin Baek Kim (Korea Atomic Energy Research Institute (KAERI)) ;
  • Min Kyu Kim (Korea Atomic Energy Research Institute (KAERI)) ;
  • Sang Young Im (Korea Atomic Energy Research Institute (KAERI)) ;
  • Jae Nam Park (Songwon University, Department of Food and Nutrition)
  • 투고 : 2023.06.12
  • 심사 : 2023.06.22
  • 발행 : 2023.06.30
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초록

The global problem of handling radioactive materials is facing limitations. Eco-friendly bioremediation methods using microorganisms are being studied. This study was conducted to screen cesium-resistant microbial strains. M1 strain was selected from the soil sample by enriched culture in R2A medium containing 100 mM CsCl. In liquid medium containing above 40 mM of CsCl, the growth of M1 was inhibited in a concentration-dependent manner. Otherwise, M1 can survive up to 80mM CsCl in solid medium although the growth rate was slow and colony size was small. M1 strain was genetically identified as a strain of the genus Acinetobacter through 16S rRNA sequencing, and radiation resistance (D10 value) of M1 was found to be 0.307 kGy. These results showed that M1 strain is highly resistant to cesium and can grow in radiation environment. It was considered that M1 strain is useful in the field of biological decontamination of cesium.

키워드

과제정보

본 연구는 과학기술정통부의 재원으로 한국원자력연구원 주요사업(523320-23) 및 한국연구재단(RS-2022-00156231)의 지원을 받아 수행되었으며, 한국원자력연구원 해체기술개발부 노창현 박사님의 연구지원에 감사드립니다.

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