Journal of Korean Medicine Rehabilitation (한방재활의학과학회지)

The society of Korean Medicine Rehabilitation (한방재활의학과학회)
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The Effect of Baekhogainsam-tang on Metabolism through Modulation of the Gut Microbiota and Gene Expression in High-Fat Diet Induced Metabolic Syndrome Animal Model

고지방식이로 유도된 대사증후군 모델 동물에서 백호가인삼탕(白虎加人參湯)의 장내미생물 및 유전자 발현 조절을 통한 대사 개선 효과

  • Min-Jin Cho (Department of Korean Medicine Rehabilitation, College of Korean Medicine, Dongguk University) ;
  • Song-Yi Han (Department of Korean Medicine Rehabilitation, College of Korean Medicine, Dongguk University) ;
  • Soo Kyoung Lim (Department of Korean Medicine Rehabilitation, College of Korean Medicine, Dongguk University) ;
  • Eun-Ji Song (Research Group of Healthcare, Korea Food Research Institute) ;
  • Young-Do Nam (Research Group of Healthcare, Korea Food Research Institute) ;
  • Hojun Kim (Department of Korean Medicine Rehabilitation, College of Korean Medicine, Dongguk University)
  • 조민진 (동국대학교 한의과대학 한방재활의학교실) ;
  • 한송이 (동국대학교 한의과대학 한방재활의학교실) ;
  • 임수경 (동국대학교 한의과대학 한방재활의학교실) ;
  • 송은지 (한국식품연구원 헬스케어연구단) ;
  • 남영도 (한국식품연구원 헬스케어연구단) ;
  • 김호준 (동국대학교 한의과대학 한방재활의학교실)
  • Received : 2023.06.20
  • Accepted : 2023.07.02
  • Published : 2023.07.31
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Abstract

Objectives We aimed to find out the improvement effect of Baekhogainsam-tang (Baihu Jia Renshen-tang, BIT) on metabolic syndrome and alteration of microbiota and gene expression. Methods We used male C57BI/6 mice and randomly assigned them into three groups. Normal control group was fed 10% kcal% fat diet, high-fat diet (HFD) group was fed 45% kcal% fat diet and 10% fructose water. BIT group was fed same diet as HFD group and treated by BIT for once daily, 6 days per week, total 8 weeks. We measured their body weight and food intake every week and performed oral glucose tolerance test 1 week before the end of the study. Then we collected the blood sample to measure triglyceride, total cholesterol, high-density lipoprotein cholesterol, insulin, and hemoglobin A1c. We harvested tissue of liver, muscle, fat, and large intestine for quantitative polymerase chain reaction (qPCR) and histopathological examination. Fresh fecal samples were collected from each animal to verify alterations of gut microbiota and we used RNA from liver tissue for microarray analysis. Results The body weight and fat weight of BIT group were reduced compared to HFD group. The qPCR markers usually up-regulated in metabolic syndrome were decreased in BIT group. Bacteroides were higher in BIT group than other groups. There were also differences in gene expressions between two groups such as Cyp3a11 and Scd1. Conclusions We could find out BIT can ameliorate metabolic syndrome and suggest its effect is related to gut microbiota composition and gene expression pattern.

Keywords

Acknowledgement

This study was supported by 'Healthcare R&D of Korean Health Industry Development Institute (HF20C0020)' from the Ministry of Health and Welfare and 'Korea Food Research Institute (E0170600-07)' from the Ministry of Science and ICT.

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