한국자원식물학회지 (Korean Journal of Plant Resources)

  • 제36권4호
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  • Pages.290-298
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  • 2023
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  • 1226-3591(pISSN)
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  • 2287-8203(eISSN)
한국자원식물학회 (The Plant Resources Society of Korea)
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닥나무 속 식물의 엽록체 유전체 기반 InDel 마커의 개발

Development of Chloroplast Genome-based Insertion/Deletion Markers in the Genus Broussonetia

  • 이은지 (충북대학교 특용식물학과) ;
  • 김윤아 (충북대학교 특용식물학과) ;
  • 이미선 (충북대학교 특용식물학과) ;
  • 김주혁 (충북대학교 특용식물학과) ;
  • 최용규 (충북대학교 산림학과) ;
  • 김정성 (충북대학교 산림학과) ;
  • 신창섭 (충북대학교 산림학과) ;
  • 이이 (충북대학교 특용식물학과)
  • Eun Jee Lee (Department of Industrial Plant Science & Technology, Chungbuk National University) ;
  • Yoon A Kim (Department of Industrial Plant Science & Technology, Chungbuk National University) ;
  • Mi Sun Lee (Department of Industrial Plant Science & Technology, Chungbuk National University) ;
  • Ju Hyeok Kim (Department of Industrial Plant Science & Technology, Chungbuk National University) ;
  • Young Kyu Choi (Department of Forest Science, Chungbuk National University) ;
  • Jung Sung Kim (Department of Forest Science, Chungbuk National University) ;
  • Chang Seob Sin (Department of Forest Science, Chungbuk National University) ;
  • Yi Lee (Department of Industrial Plant Science & Technology, Chungbuk National University)
  • 투고 : 2022.11.01
  • 심사 : 2023.04.28
  • 발행 : 2023.08.01
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초록

본 연구에서는 닥나무 속 식물에 대한 InDel 마커를 개발하였다. 전국의 닥나무 속 식물 22개체를 수집하였고, 수집한 닥나무 속 식물 중 6개체를 차세대염기서열 분석(NGS)을 실시하였다. NGS를 통하여 얻은 염기서열 정보를 기존에 발표되었던 닥나무 엽록체 서열과 비교하여 InDel 마커 후보를 선발하였다. 선발한 마커 후보를 수집된 닥나무 속 식물에 적용하여 마커의 특성 검정을 통해 총5개의 엽록체 기반 마커를 개발하였다. 개발된 InDel 마커를 22개의 유전자원에 적용한 후 군집 분석을 실시한 결과, 총5개의 그룹으로 나뉘었다. 본 연구에서 개발된 마커들은 닥나무 속의 육종이나 종 판별에 활용할 수 있을 것이라 판단된다.

Several members of the genus Broussonetia are woody plants with high-quality cellulose fibers and are used to make a traditional type of Korean paper known as Hanji. Three of these species, Broussonetia kazinoki, Broussonetia monoica, and Broussonetia papyrifera, are found in the Korean Peninsula. Because it is challenging to distinguish different Broussonetia species based on morphology alone, we have developed a set of insertion/deletion (InDel) markers for genetic identification of these species. From twenty-two Broussonetia samples collected throughout Korea, we selected six for next-generation sequencing analysis. InDel marker candidates were identified by comparing this sequence information with the B. kazinoki chloroplast genome sequence. The marker candidates were used to screen the genomes of the twenty-two Broussonetia plants, and five useful chloroplast-based InDel markers were identified. Detailed genotyping using these five markers showed that the twenty-two plants of the genus Broussonetia could be clustered into five groups, verifying that the markers developed here can be used for breeding, identification, and analysis of species in the genus Broussonetia.

키워드

과제정보

본 논문은 2022년도 교육부의 재원으로 한국연구재단의 지원을 받아 수행된 지자체-대학 협력기반 지역혁신 사업의 결과입니다.(2021RIS-001)

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