Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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Effect of Temperature Conditions and Chemical Treatments on Seed Germination of Pseudolysimachion kiusianum var. diamantiacum (Nakai) T.Yamaz.

봉래꼬리풀의 종자 발아에 미치는 온도 조건과 화학적 처리의 영향

  • Dong-Hak Kim (DMZ Botanic Garden, Korea National Arboretum) ;
  • Young-Eun Kim (DMZ Botanic Garden, Korea National Arboretum) ;
  • Seungju Jo (Department of Forest Environmental System, Kangwon National University) ;
  • Jong-Won Lee (DMZ Botanic Garden, Korea National Arboretum) ;
  • Sang-Jun Kim (DMZ Botanic Garden, Korea National Arboretum)
  • 김동학 (국립수목원 DMZ산림생물자원보전과) ;
  • 김영은 (국립수목원 DMZ산림생물자원보전과) ;
  • 조승주 (강원대학교 산림환경시스템학과) ;
  • 이종원 (국립수목원 DMZ산림생물자원보전과) ;
  • 김상준 (국립수목원 DMZ산림생물자원보전과)
  • Received : 2023.03.03
  • Accepted : 2023.04.16
  • Published : 2023.08.01
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Abstract

The germination characteristics of Pseudolysimachion kiusianum var. diamantiacum (Nakai) T.Yamaz., a rare and endemic plant designated by the Korea Forest Service, were investigated according to light conditions, temperature, and pretreatment. As a result of the germination experiment according to light conditions, it was determined that P. kiusianum is a photoblastic seed that does not germinate at all in dark conditions. The optimum germination temperature of the seeds was found to be 20-25℃, considering the final germination rate and germination time. Three growth regulators (IAA, GA3, kinetin) and two inorganic salts (KNO3, KCl) were pretreated to improve the germination rate of P. kiusianum seeds. The growth regulators IAA and kinetin had no significant effect on improving the germination rate of P. kiusianum seeds. On the other hand, GA3 significantly increased the final germination percentage and germination rate regardless of the concentration, especially the treatment of more than 500 mg·L-1 at 20℃ was more than 4 times more effective than the untreated. The inorganic salts KNO3 and KCl had no significant effect on the seeds of P. kiusianum at low concentrations, but at higher concentrations (40 mM and 300 mM, respectively), they improved the germination rate and germination age by 2 times compared to the untreated. The results of this study will be useful for the mass propagation of P. kiusianum, which has the potential to be utilized as a native plant for restoration.

산림청 지정 희귀·특산식물 봉래꼬리풀(Pseudolysimachion kiusianum var. diamantiacum (Nakai) T.Yamaz.)을 대상으로 광조건, 온도와 전처리에 따른 발아 특성을 조사하였다. 광조건에 따른 발아실험결과, 봉래꼬리풀은 암조건에서 전혀 발아하지 않는 광발아성 종자로 판단되었다. 봉래꼬리풀의 최적 발아온도는 최종 발아율과 발아세를 고려하여 20-25℃임을 확인하였다. 봉래꼬리풀 종자의 발아율 향상을 위해 생장조절제 3종(IAA, GA3, kinetin)과 무기염류 2종(KNO3, KCl)을 전처리하였다. 생장조절제 IAA와 kinetin은 봉래꼬리풀 종자의 발아율 향상에 유의미한 영향을 미치지 못했다. 반면 GA3는 농도와 관계없이 최종 발아율과 발아세를 유의미한 수준으로 증가시켰고, 특히 20℃에서 500 mg·L-1 이상 처리한 것은 무처리구에 비해 4배 이상 효과적이었다. 무기염류 KNO3과 KCl는 저농도에서 봉래꼬리풀 종자에 유의미한 영향을 미치지 못했지만 고농도(각40 mM, 300 mM) 이상에서는 발아율과 발아세를 무처리구에 비해 1.5배 이상 향상시켰다. 본 연구의 결과는 복원용 자생식물로 활용 가치가 있는 봉래꼬리풀의 대량증식에 유용한 자료가 될 것이다.

Keywords

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