The Journal of Internal Korean Medicine (대한한방내과학회지)

The Society of Internal Korean Medicine (대한한방내과학회)
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Effects of GGX on an Ovalbumin-induced Asthma Mice Model

Ovalbumin으로 유발된 천식 동물모델에서 GGX의 효과

  • Tae-hyeon Kim (Division of Respiratory System, Dept. of Internal Medicine, College of Korean Medicine, Daejeon University) ;
  • Won-kyung Yang (Division of Respiratory System, Dept. of Internal Medicine, College of Korean Medicine, Daejeon University) ;
  • Su-won Lee (Division of Respiratory System, Dept. of Internal Medicine, College of Korean Medicine, Daejeon University) ;
  • Seong-cheon Woo (Division of Respiratory System, Dept. of Internal Medicine, College of Korean Medicine, Daejeon University) ;
  • Seung-hyung Kim (Institute of Traditional Medicine and Bioscience, Daejeon University) ;
  • Yang-chun Park (Division of Respiratory System, Dept. of Internal Medicine, College of Korean Medicine, Daejeon University)
  • 김태현 (대전대학교 한의과대학 폐계내과학교실) ;
  • 양원경 (대전대학교 한의과대학 폐계내과학교실) ;
  • 이수원 (대전대학교 한의과대학 폐계내과학교실) ;
  • 우성천 (대전대학교 한의과대학 폐계내과학교실) ;
  • 김승형 (대전대학교 동서생명과학연구원) ;
  • 박양춘 (대전대학교 한의과대학 폐계내과학교실)
  • Received : 2023.03.08
  • Accepted : 2023.06.27
  • Published : 2023.06.30
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Abstract

Objective: The purpose of this study is to evaluate the effects of GGX on an ovalbumin (OVA)-induced asthma mice model. Methods: Balb/c mice were challenged with OVA and then treated with three concentrations of GGX (100, 200, and 400 mg/kg). After sacrifice, the bronchoalveolar lavage fluid (BALF) or lungs of the mice were analyzed by fluorescence-activated cell sorting, ELISA, real-time PCR, H&E, Masson's trichrome, PAS and AB-PAS staining, and immunohistofluorescence staining. Results: GGX significantly inhibited the increase of total cells, immune cells (lymphocyte, neutrophils, macrophage, CD4+, CD8+, CD4+CD69+, CD62L-CD44high+, Gr-1+SiglecF-), and the expression of cytokines (IL-4, IL-5, IL-13, IFN-γ) in BALF. It also significantly inhibited the increase of total cells, immune cells (lymphocyte, neutrophils, eosinophil/macrophage, CD3+, CD19+, CD3+CD193+, CD4+, CD8+, CD4+CD69+, CD62L-CD44high+, and Gr-1+SiglecF-), and the expression of IL-13, TARC, and MCP-1 in lung tissue. GGX decreased the severity of histological lung injury and the expressions of STAT3 and GATA3. Conclusion: This study suggests the probability of using GGX for the treatment of asthma by inhibiting inflammatory immune response.

Keywords

Acknowledgement

이 논문은 2021년도 보건복지부 한의기반융합기술개발사업(HI21C1339) 및 2022년도 한국연구재단의 기초연구사업(과제번호: NRF-2022R1A2C1007165)의 지원을 받아 수행된 연구임.

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