Journal of Species Research

The National Institute of Biological Resources (국립생물자원관)
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A report of sixteen unrecorded haloarchaea species in Korea, isolated from a solar saltern

  • Chi Young Hwang (Department of Bioengineering and Nano-Bioengineering, Incheon National University) ;
  • Eui-Sang Cho (Department of Bioengineering and Nano-Bioengineering, Incheon National University) ;
  • Dong-Hyun Jung (Microorganism Resources Division, National Institute of Biological Resources) ;
  • Ki-Eun Lee (Microorganism Resources Division, National Institute of Biological Resources) ;
  • In-Tae Cha (Microorganism Resources Division, National Institute of Biological Resources) ;
  • Won-Jae Chi (Microorganism Resources Division, National Institute of Biological Resources) ;
  • Myung-Ji Seo (Department of Bioengineering and Nano-Bioengineering, Incheon National University)
  • Received : 2022.12.30
  • Accepted : 2023.07.06
  • Published : 2023.08.04
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Abstract

In July 2018, solar saltern samples were collected from Siheung, Gyeonggi-do Province to obtain unrecorded haloarchaea in Korea. The samples were suspended in a 20% NaCl (w/v) solution, and serial dilution was performed in fresh DB Characterization media No. 2. The strains isolated in this study showed at least 98.7% sequence similarity or more compared to the previously reported. Finally, 16 haloarchaeal strains, which were not reported in Korea but validly published under the International Code of Nomenclature of Prokaryotes (ICNP), were obtained from a solar saltern in Siheung. These 16 isolates were allocated to the orders Halobacteriales and Haloferacales. The 10 Halobacteriales strains were classified into the family Halobacteriaceae and Haloarculaceae. Each family belonged to three genera, respectively. The other six Haloferacales belonged to the families Haloferacaceae and Halorubraceae. Each family belonged to genus genus, respectively. Collectively, the unrecorded haloarchaeal strains belonged to two orders, four families, and eight genera. During the research, the possibility of discovering previously unknown species in domestic solar saltern was established. Gram-staining, cell morphology, physiological and basic biochemical parameters, and phylogenetic analysis were all performed in this study and are described in detail for each strain.

Keywords

Acknowledgement

This work was supported by grants from the National Institute of Biological Resources funded by the Ministry of Environment (No. NIBR202203112), Republic of Korea.

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