치위생과학회지 (Journal of dental hygiene science)

  • 제23권2호
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  • Pages.103-111
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  • 2023
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  • 1598-4478(pISSN)
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  • 2233-7679(eISSN)
한국치위생과학회 (The Korean Society of Dental Hygiene Science)
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Effects of Lactobacillus casei and Aggregatibactor actinomycetemcomitans against Streptococcus mutans according to the Concentration of Sucrose

  • Soon-Jeong Jeong (Department of Dental Hygiene & Institute of Basic Science for Well-Aging, College of Health Science, Youngsan University)
  • 투고 : 2023.05.25
  • 심사 : 2023.06.05
  • 발행 : 2023.06.30
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초록

Background: Some studies confirm the reduction of the number of Streptococcus mutans in saliva and dental plaque by Lactobacillus, however, these effects are not always confirmed in in vitro and clinical studies, and only the risk of dental caries has been reported. Our in vitro study aimed to reveal microbial and biochemical changes in the single cultures of S. mutans, Lactobacillus casei and Aggregatibactor actinomycetemcomitans and co-cultures of S. mutans and L. casei or A. actinomycetemcomitans according to sucrose concentration. We also aimed to confirm the anti-oral bacterial and anti-biofilm activities of L. casei and A. actinomycetemcomitans against S. mutans according to sucrose concentration. Methods: S. mutans (KCCM 40105), L. casei (KCCM 12452), and A. actinomycetemcomitans (KCTC 2581) diluted to 5×106 CFU/ml were single cultured, and L. casei or A. actinomycetemcomitans applied at concentrations of 10%, 20%, 30% and 40% to S. mutans were co-cultured with selective medium containing 0%, 1% and 5% sucrose at 36.5℃ for 24 hours. Measurements of bacterial growth value and acid production, disk diffusion and biofilm formation assays were performed. Results: In the medium containing sucrose, the bacterial growth and biofilm formation by S. mutans, L. casei, and A. actinomycetemcomitans were increased. In contrast, 30% and 40% of L. casei in the medium containing 0% sucrose showed both anti-oral bacterial and anti-biofilm activities. This implies that L. casei can be used as probiotic therapy to reduce S. mutans in a 0% sucrose environment. Conclusion: The concentration of sucrose in the oral environment is important for the control of pathogenic bacteria that cause dental caries and periodontitis. To apply probiotic therapy using L. casei for S. mutans reduction, the concentration of sucrose must be considered.

키워드

과제정보

This work was supported by Youngsan University Research Fund of 2022.

참고문헌

  1. Jothika M, Vanajassun PP, Someshwar B: Effectiveness of probiotic, chlorhexidine and fluoride mouthwash against Streptococcus mutans- randomized, single-blind, in vivo study. J Int Soc Prev Community Dent 5(Suppl 1): S44-S48, 2015. https://doi.org/10.4103/2231-0762.156153
  2. Yang SY, Lee SH, Park OB, et al.: Antibacterial effects of tea tree oil and mastic oil to Streptococcus mutans. J Dent Hyg Sci 23: 51-59, 2023. https://doi.org/10.17135/jdhs.2023.23.1.51
  3. Monchois V, Willemot RM, Monsan P: Glucansucrases: mechanism of action and structure-function relationships. FEMS Microbiol Rev 23: 131-151, 1999. https://doi.org/10.1111/j.1574-6976.1999.tb00394.x
  4. Cvitkovitch DG, Li YH, Ellen RP: Quorum sensing and biofilm formation in Streptococcal infections. J Clin Invest 112: 1626-1632, 2003. https://doi.org/10.1172/JCI20430
  5. Kang MS, Kang IC, Kim SM, Lee HC, Oh JS: Effect of Leuconostoc spp. on the formation of Streptococcus mutans biofilm. J Microbiol 45: 291-296, 2007.
  6. Nunpan S, Suwannachart C, Wayakanon K: Effect of prebiotics-enhanced probiotics on the growth of Streptococcus mutans. Int J Microbiol 2019: 4623807, 2019. https://doi.org/10.1155/2019/4623807
  7. Mohanty R, Nazareth B, Shrivastava N: The potential role of probiotics in periodontal health. RSBO 9: 85-88,
  8. Vuotto C, Longo F, Donelli G: Probiotics to counteract biofilm-associated infections: promising and conflicting data. Int J Oral Sci 6: 189-194, 2014. https://doi.org/10.1038/ijos.2014.52
  9. Gu M, Cho JH, Suh JW, Cheng J: Potential oral probiotic Lactobacillus pentosus MJM60383 inhibits Streptococcus mutans biofilm formation by inhibiting sucrose decomposition. J Oral Microbiol 15: 2161179, 2022. https://doi.org/10.1080/20002297.2022.2161179
  10. Ahmed A, Dachang W, Lei Z, Jianjun L, Juanjuan Q, Yi X: Effect of Lactobacillus species on Streptococcus mutans biofilm formation. Pak J Pharm Sci 27(5 Spec no): 1523-1528, 2014.
  11. Bonifait L, Chandad F, Grenier D: Probiotics for oral health: myth or reality? J Can Dent Assoc 75: 585-590,
  12. Wasfi R, Abd El-Rahman OA, Zafer MM, Ashour HM: Probiotic Lactobacillus sp. inhibit growth, biofilm formation and gene expression of caries-inducing Streptococcus mutans. J Cell Mol Med 22: 1972-1983, 2018. https://doi.org/10.1111/jcmm.13496
  13. Nguyen T, Brody H, Radaic A, Kapila Y: Probiotics for periodontal health- current molecular findings. Periodontol 2000 87: 254-267, 2021. https://doi.org/10.1111/prd.12382
  14. Kapoor A, Malhotra R, Grover V, Grover D: Systemic antibiotic therapy in periodontics. Dent Res J (Isfahan) 9: 505-515, 2012. https://doi.org/10.4103/1735-3327.104866
  15. Mahasneh SA, Mahasneh AM: Probiotics: a promising role in dental health. Dent J (Basel) 5: 26, 2017. https://doi.org/10.3390/dj5040026
  16. Gruner D, Paris S, Schwendicke F: Probiotics for managing caries and periodontitis: systematic review and meta-analysis. J Dent 48: 16-25, 2016. https://doi.org/10.1016/j.jdent.2016.03.002
  17. Stecksen-Blicks C, Sjostrom I, Twetman S: Effect of long-term consumption of milk supplemented with probiotic lactobacilli and fluoride on dental caries and general health in preschool children: a cluster-randomized study. Caries Res 43: 374-381, 2009. https://doi.org/10.1159/000235581
  18. Hasslof P, West CE, Videhult FK, Brandelius C, StecksenBlicks C: Early intervention with probiotic Lactobacillus paracasei F19 has no long-term effect on caries experience. Caries Res 47: 559-565, 2013. https://doi.org/10.1159/000350524
  19. Schwendicke F, Korte F, Dorfer CE, Kneist S, Fawzy El-Sayed K, Paris S: Inhibition of Streptococcus mutans growth and biofilm formation by probiotics in vitro. Caries Res 51: 87-95, 2017. https://doi.org/10.1159/000452960
  20. Taipale T, Pienihakkinen K, Alanen P, Jokela J, Soderling E: Administration of Bifidobacterium animalis subsp. lactis BB-12 in early childhood: a post-trial effect on caries occurrence at four years of age. Caries Res 47: 364-372, 2013. https://doi.org/10.1159/000348424
  21. Somanah J, Bourdon E, Bahorun T, Aruoma OI: The inhibitory effect of a fermented papaya preparation on growth, hydrophobicity, and acid production of Streptococcus mutans, Streptococcus mitis, and Lactobacillus acidophilus: its implications in oral health improvement of diabetics. Food Sci Nutr 1: 416-421, 2013. https://doi.org/10.1002/fsn3.55
  22. O'Toole GA, Pratt LA, Watnick PI, Newman DK, Weaver VB, Kolter R: Genetic approaches to study of biofilms. Methods Enzymol 310: 91-109, 1999. https://doi.org/10.1016/s0076-6879(99)10008-9
  23. Jeong MJ, Lim DS, Heo KW, Jeong SJ: Anti-oral microbial activity and anti-inflammatory effects of rosmarinic acid in lipopolysaccharide-stimulated MC3T3-E1 osteoblastic cells on a titanium surface. J Dent Hyg Sci 20: 221-229, 2020. https://doi.org/10.17135/jdhs.2020.20.4.221
  24. Cai JN, Jung JE, Lee MH, Choi HM, Jeon JG: Sucrose challenges to Streptococcus mutans biofilms and the curve fitting for the biofilm changes. FEMS Microbiol Ecol 94: fiy091, 2018. https://doi.org/10.1093/femsec/fiy091
  25. Soderling EM, Marttinen AM, Haukioja AL: Probiotic lactobacilli interfere with Streptococcus mutans biofilm formation in vitro. Curr Microbiol 62: 618-622, 2011. https://doi.org/10.1007/s00284-010-9752-9
  26. Ishihara K, Miyakawa H, Hasegawa A, Takazoe I, Kawai Y: Growth inhibition of Streptococcus mutans by cellular extracts of human intestinal lactic acid bacteria. Infect Immun 49: 692-694, 1985. https://doi.org/10.1128/iai.49.3.692-694.1985
  27. Nikawa H, Makihira S, Fukushima H, et al.: Lactobacillus reuteri in bovine milk fermented decreases the oral carriage of mutans streptococci. Int J Food Microbiol 95: 219-223, 2004. https://doi.org/10.1016/j.ijfoodmicro.2004.03.006
  28. Villavicencio J, Villegas LM, Arango MC, Arias S, Triana F: Effects of a food enriched with probiotics on Streptococcus mutans and Lactobacillus spp. salivary counts in preschool children: a cluster randomized trial. J Appl Oral Sci 26: e20170318, 2018. https://doi.org/10.1590/1678-7757-2017-0318
  29. Lin X, Chen X, Tu Y, Wang S, Chen H: Effect of probiotic lactobacilli on the growth of Streptococcus Mutans and multispecies biofilms isolated from children with active caries. Med Sci Monit 23: 4175-4181, 2017. https://doi.org/10.12659/msm.902237
  30. Tahmourespour A, Salehi R, Kasra Kermanshahi R: Lactobacillus acidophilus-derived biosurfactant effect on GTFB and GTFC expression level in Streptococcus mutans biofilm cells. Braz J Microbiol 42: 330-339, 2011. https://doi.org/10.1590/S1517-83822011000100042
  31. Koll P, Mandar R, Marcotte H, Leibur E, Mikelsaar M, Hammarstrom L: Characterization of oral lactobacilli as potential probiotics for oral health. Oral Microbiol Immunol 23: 139-147, 2008. https://doi.org/10.1111/j.1399-302X.2007.00402.x
  32. Terai T, Okumura T, Imai S, et al.: Screening of probiotic candidates in human oral bacteria for the prevention of dental disease. PLoS One 10: e0128657, 2015. https://doi.org/10.1371/journal.pone.0128657