Clinical and Experimental Vaccine Research

  • 제12권1호
  • /
  • Pages.32-46
  • /
  • 2023
  • /
  • 2287-3651(pISSN)
  • /
  • 2287-366X(eISSN)
대한백신학회 (The Korean Vaccine Society)
권호 표지
DOI QR코드

DOI QR Code

Comparative evaluation of gold nanoparticles and Alum as immune enhancers against rabies vaccine and related immune reactivity, physiological, and histopathological alterations: in vivo study

  • Rehab Essam El-Din El-Hennamy (Zoology and Entomology Department, Faculty of Science, Helwan University) ;
  • Sahar Mohamed Mahmoud (Zoology Department, Faculty of Science, Cairo University) ;
  • Nabil Ahmed El-Yamany (Zoology and Entomology Department, Faculty of Science, Helwan University) ;
  • Hanaa Hassan Hassanein (The Holding Company for Production of Vaccines, Sera and Drugs (VACSERA-EgyVac)) ;
  • Mohamed Elsayed Amer (Histology Department, Faculty of Medicine, Al-Azhar University) ;
  • Aly Fahmy Mohamed (The Holding Company for Production of Vaccines, Sera and Drugs (VACSERA-EgyVac))
  • 투고 : 2021.11.11
  • 심사 : 2023.01.17
  • 발행 : 2023.01.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Purpose: The present study aimed to compare the immune-enhancing potential of gold nanoparticles (AuNPs) to Alum against rabies vaccine and the related immunological, physiological, and histopathological effects. Materials and Methods: Alum and AuNPs sole and in combination with rabies vaccine were used at 0.35 mg/mL and 40 nM/mL, respectively. Rats used were categorized into six groups (20/each): control rats, rabies vaccine, aluminum phosphate gel, rabies vaccine adsorbed to Alum, AuNPs, and rabies vaccine adjuvant AuNPs. Results: Liver and kidney functions were in the normal range after AuNPs and Alum adjuvanted vaccine compared to control. Interleukin-6 and interferon-γ levels were significantly increased in groups immunized with Alum and AuNPs adjuvanted vaccine, the peak level was in the case of AuNP adjuvanted vaccine on the 14th day. Ninety days post-vaccination, total immunoglobulin G (IgG) against adjuvanted rabies vaccine showed a significantly elevated antirabies IgG with AuNPs and Alum adsorbed vaccine compared with unadjuvanted one. The total antioxidant capacity, malondialdehyde (MDA) levels, superoxide dismutase, and glutathione peroxidase activities were significantly increased post-adjuvanted AuNPs adjuvanted vaccine vaccination than in Alum adsorbed vaccine, while MDA was significantly decreased. The histopathological examination revealed detectable alterations post-AuNPs and Alum adjuvanted vaccine immunization compared with liver and kidney profiles post-administration of unadjuvanted and non-immunized groups, meanwhile, splenic tissue revealed hyperplasia of lymphoid follicles indicating increased immune reactivity. Conclusion: The AuNPs are promising enhancers of the immune response as Alum, and the undesirable effects of AuNPs could be managed by using suitable sizes, shapes, and concentrations.

키워드

참고문헌

  1. World Health Organization. WHO expert consultation on rabies [Internet]. Geneva: World Health Organization; 2016 [cited 2021 Nov 5]. Available from: http://www.who.int/mediacentre/factsheets/fs099/en/.
  2. Francois G, Duclos P, Margolis H, et al. Vaccine safety controversies and the future of vaccination programs. Pediatr Infect Dis J 2005;24:953-61.
  3. Mohan T, Verma P, Rao DN. Novel adjuvants & delivery vehicles for vaccines development: a road ahead. Indian J Med Res 2013;138:779-95.
  4. Poon C, Patel AA. Organic and inorganic nanoparticle vaccines for prevention of infectious diseases. Nano Express 2020;1:012001.
  5. Gupta RK. Aluminum compounds as vaccine adjuvants. Adv Drug Deliv Rev 1998;32:155-72.
  6. el-Karamany RM. Production in Vero cells of an inactivated rabies vaccine from strain FRV/K for animal and human use. Acta Virol 1987;31:321-8.
  7. Mohamed FA, El-Kaschef AH, Reda IM, Tantawey HM, Hassen RA. Biological studies for the importance of Rift Valley fever vaccine [dissertation]. Ismailia: Suez Canal University; 1997.
  8. Engvall E, Perlmann P. Enzyme-linked immunosorbent assay (ELISA) quantitative assay of immunoglobulin G. Immunochemistry 1971;8:871-4.
  9. Drury RA, Wallington EA. Preparation and fixation of tissues. In: Carleton HM, Drury RA, Wallington EA, editors. Carleton's histological technique. 5th ed. Oxford: Oxford University Press; 1980. p. 41-54.
  10. Gomez KA, Gomez AA. Statistical procedure for agriculture research. 2nd ed. Singapore: John Wiley & Sons Inc.; 1984. p. 680-8.
  11. Rose WA, Okragly AJ, Patel CN, Benschop RJ. IL-33 released by alum is responsible for early cytokine production and has adjuvant properties. Sci Rep 2015;5:13146.
  12. Pincus MR, Schaffer JA. Assessment of liver function. In: Henry JB, editor. Clinical diagnosis and management by laboratory methods. 20th ed. Philadelphia (PA): W.B. Saunders Company; 2001. p. 253-67.
  13. Orabi SH, Mansour DA, Fathalla SI, Gadallah SM, ElDin AA, Abdoon AS. Effects of administration of 10 nm or 50 nm gold nanoparticles (AuNPs) on blood profile, liver and kidney functions in male albino rats. Indian J Biochem Biophys 2020;57:486-93.
  14. Sriwijitalai W, Wiwanitkit V. Rabies, rabies vaccine, and renal failure: clinical issues. Saudi J Kidney Dis Transpl 2019;30:560-3.
  15. Abdelhalim MA, Abdelmottaleb Moussa SA. The gold nanoparticle size and exposure duration effect on the liver and kidney function of rats: in vivo. Saudi J Biol Sci 2013;20:177-81.
  16. Lasagna-Reeves C, Gonzalez-Romero D, Barria MA, et al. Bioaccumulation and toxicity of gold nanoparticles after repeated administration in mice. Biochem Biophys Res Commun 2010;393:649-55.
  17. De Jong WH, Hagens WI, Krystek P, Burger MC, Sips AJ, Geertsma RE. Particle size-dependent organ distribution of gold nanoparticles after intravenous administration. Biomaterials 2008;29:1912-9.
  18. Cruz-Tapias P, Agmon-Levin N, Israeli E, Anaya JM, Shoenfeld Y. Autoimmune (auto-inflammatory) syndrome induced by adjuvants (ASIA): animal models as a proof of concept. Curr Med Chem 2013;20:4030-6.
  19. Nies I, Hidalgo K, Bondy SC, Campbell A. Distinctive cellular response to aluminum based adjuvants. Environ Toxicol Pharmacol 2020;78:103404.
  20. Nam NH, Luong NH. Nanoparticles: synthesis and applications. In: Grumezescu V, Grumezescu AM, editors. Materials for biomedical engineering: inorganic micro and nanostructures. Amsterdam: Elsevier Inc.; 2019. p. 211-40.
  21. Niikura K, Matsunaga T, Suzuki T, et al. Gold nanoparticles as a vaccine platform: influence of size and shape on immunological responses in vitro and in vivo. ACS Nano 2013;7:3926-38.
  22. Dykman LA, Khlebtsov NG. Immunological properties of gold nanoparticles. Chem Sci 2017;8:1719-35.
  23. Howe RC, Dhiman N, Ovsyannikova IG, Poland GA. Induction of CD4 T cell proliferation and in vitro Th1-like cytokine responses to measles virus. Clin Exp Immunol 2005;140:333-42.
  24. McKee AS, Munks MW, MacLeod MK, et al. Alum induces innate immune responses through macrophage and mast cell sensors, but these sensors are not required for alum to act as an adjuvant for specific immunity. J Immunol 2009;183:4403-14.
  25. Zhou Q, Zhang Y, Du J, et al. Different-sized gold nanoparticle activator/antigen increases dendritic cells accumulation in liver-draining lymph nodes and CD8+ T cell responses. ACS Nano 2016;10:2678-92.
  26. Paweska JT, Burt FJ, Swanepoel R. Validation of IgG-sandwich and IgM-capture ELISA for the detection of antibody to Rift Valley fever virus in humans. J Virol Methods 2005;124:173-81.
  27. Hogenesch H. Mechanism of immunopotentiation and safety of aluminum adjuvants. Front Immunol 2013;3:406.
  28. Lee CH, Syu SH, Chen YS, et al. Gold nanoparticles regulate the blimp1/pax5 pathway and enhance antibody secretion in B-cells. Nanotechnology 2014;25:125103.
  29. Soliman MG, Mohamed AF, El sayed RA, Elqasem AA. Immunohistochemical and histological changes in the spleen induced by gold nanoparticles as alternative adjuvant against Rift Vally fever virus. Eur J Biomed Pharm Sci 2017;4:537-42.
  30. Kakarla P, Vadluri G, Reddy Kesireddy S. Response of hepatic antioxidant system to exercise training in aging female rat. J Exp Zool A Comp Exp Biol 2005;303:203-8.
  31. Abdelhalim MA, Al-Ayed MS, Moussa SA. The effects of intraperitoneal administration of gold nanoparticles size and exposure duration on oxidative and antioxidants levels in various rat organs. Pak J Pharm Sci 2015;28(2 Suppl):705-12.
  32. Niedernhofer LJ, Daniels JS, Rouzer CA, Greene RE, Marnett LJ. Malondialdehyde, a product of lipid peroxidation, is mutagenic in human cells. J Biol Chem 2003;278:31426-33.
  33. Barathmanikanth S, Kalishwaralal K, Sriram M, et al. Antioxidant effect of gold nanoparticles restrains hyperglycemic conditions in diabetic mice. J Nanobiotechnology 2010;8:16.
  34. Muselin F, Garban Z, Cristina RT, et al. Homeostatic changes of some trace elements in geriatric rats in the condition of oxidative stress induced by aluminum and the beneficial role of resveratrol. J Trace Elem Med Biol 2019;55:136-42.
  35. Bogdanovic M, Janeva AB, Bulat P. Histopathological changes in rat liver after a single high dose of aluminium. Arh Hig Rada Toksikol 2008;59:97-101.
  36. Doudi M, Setorki M. The effect of gold nanoparticle on renal function in rats. Nanomed J 2014;1:171-9.
  37. Fu Y, Zhang Y, Chang X, et al. Systemic immune effects of titanium dioxide nanoparticles after repeated intratracheal instillation in rat. Int J Mol Sci 2014;15:6961-73.
  38. Crane GM, Liu YC, Chadburn A. Spleen: development, anatomy and reactive lymphoid proliferations. Semin Diagn Pathol 2021;38:112-24.
  39. Todorovic-Rakovic N, Whitfield JR. Between immunomodulation and immunotolerance: the role of IFNγ in SARS-CoV-2 disease. Cytokine 2021;146:155637.