Journal of Food Hygiene and Safety (한국식품위생안전성학회지)

The Korean Society of Food Hygiene and Safety (한국식품위생안전성학회)
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Monitoring of Melatonin Contents in Nuts, Seeds, and Beans in Gyeonggi-Do

경기도 내 유통 중 견과종실류 등의 멜라토닌 함량 조사

  • Yu Na Song (Food Analysis Team in North Branch, Gyeonggi-do Institute of Health & Environment) ;
  • Hae Geun Hong (Food Analysis Team in North Branch, Gyeonggi-do Institute of Health & Environment) ;
  • Yeon Ok Kwon (Food Analysis Team in North Branch, Gyeonggi-do Institute of Health & Environment) ;
  • Jin Ok Ha (Food Analysis Team in North Branch, Gyeonggi-do Institute of Health & Environment) ;
  • Hyeon Ji Kim (Food Analysis Team in North Branch, Gyeonggi-do Institute of Health & Environment) ;
  • Myeong Jin Son (Food Analysis Team in North Branch, Gyeonggi-do Institute of Health & Environment) ;
  • Jeong Hwa Park (Food Analysis Team in North Branch, Gyeonggi-do Institute of Health & Environment) ;
  • Bo Yeon Kweon (Food Analysis Team in North Branch, Gyeonggi-do Institute of Health & Environment)
  • 송유나 (경기도 보건환경연구원 북부지원 식품분석팀) ;
  • 홍해근 (경기도 보건환경연구원 북부지원 식품분석팀) ;
  • 권연옥 (경기도 보건환경연구원 북부지원 식품분석팀) ;
  • 하진옥 (경기도 보건환경연구원 북부지원 식품분석팀) ;
  • 김현지 (경기도 보건환경연구원 북부지원 식품분석팀) ;
  • 손명진 (경기도 보건환경연구원 북부지원 식품분석팀) ;
  • 박정화 (경기도 보건환경연구원 북부지원 식품분석팀) ;
  • 권보연 (경기도 보건환경연구원 북부지원 식품분석팀)
  • Received : 2023.05.04
  • Accepted : 2023.06.01
  • Published : 2023.06.30
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Abstract

Nuts are essential components of a healthy diet as they provide nutritional value and bioactive components. Melatonin, is a hormone secreted from the pineal gland of the brain that prevents oxidative damage in various tissues, and also found in plants. This study presents a validation method for extracting and quantitatively analyzing melatonin in nuts, seeds, and beans in Gyeonggi-do; the method utilized chromatographic techniques and optimized extraction procedures, considering the high oil content of nuts. The average content of melatonin in nuts, seeds, and beans was 1200.83 (409.76-2223.56), 934.83 (454.10-1736.60), and 616.46 (494.70-825.12) pg/g, respectively. Melatonin content was higher in the kernel with pellicle than that in the kernel alone in walnuts and chestnuts. Furthermore, the presence of melatonin was lower in newly harvested walnuts, chestnuts, and peanuts than in those stored after being harvested the previous year.

견과류는 영양가가 우수하고 다양한 기능성 생리활성을 가져 건강한 식단에 중요한 요소이다. 멜라토닌은 뇌의 송과선에서 분비되는 호르몬으로 각종 조직의 산화적 손상을 방지하는 것으로 알려져 있으며, 식물체에서도 중요한 역할을 한다. 본 연구는 견과류의 멜라토닌 추출 및 정량을 위한 분석법을 마련하였고, 견과류의 높은 지방함량을 고려한 최적화된 추출 절차를 이용하여 경기도에서 유통되는 견과종실류 등의 멜라토닌 함량을 조사하였다. 땅콩 또는 견과류, 유지종실류 및 두류의 평균 멜라토닌 함량은 각각 1200.83(409.76-2223.56), 934.83 (454.10-1736.60), 616.46 (494.70-825.12) pg/g으로 나타났다. 호두와 밤의 경우 속껍질을 포함한 반탈각 상태와 과실만 있는 경우의 멜라토닌 함량을 비교한 결과, 반탈각 상태에서 멜라토닌 함량이 유의적으로 높았다. 또한 호두, 밤 및 땅콩의 경우 2022년 재배된 햇 견과보다 2021년 재배 후 저장된 견과에서 멜라토닌 함량이 유의적으로 높았다. 이러한 결과는 견과종실류 등의 멜라토닌 함량이 높아 우수한 기능성 식품임을 시사하였다.

Keywords

References

  1. Alasalvar, C., Shahidi, F., 2008. Tree nuts: Composition, phytochemicals, and health effects, 1st ed. CRC Press, Boca Raton, FL, USA, pp. 244-247. 
  2. Ministry of Food and Drug Safety, (2023, June 1). Food Code, https://various.foodsafetykorea.go.kr/fsd/#/ext/Document/FC?searchNm=%EC%9B%90%EB%A3%8C&item-Code=FC0A002001002A014 
  3. Phillips, K.M., Ruggio, D.M., Ashraf-Khorassani, M., Phytosterol composition of nuts and seeds commonly consumed in the United States. J. Agric. Food Chem., 53, 9436-9445 (2005).  https://doi.org/10.1021/jf051505h
  4. Albert, C.M., Gaziano, J.M., Willett, W.C., Manson, J.E., Nut consumption and decreased risk of sudden cardiac death in the physicians' health study. Arc. Int. Med., 162, 1382-1387 (2002).  https://doi.org/10.1001/archinte.162.12.1382
  5. Ellsworth, J.L., Kushi, L.H., Folsom, A.R., Frequent nut intake and risk of death from coronary heart disease and all causes in postmenopausal women: The Iowa women's health study. Nutrition, Metabolism, and Cardiovascular Diseases: NMCD, 11, 372-377 (2001). 
  6. Arab, L., Ang, A., A cross sectional study of the association between walnut consumption and cognitive function among adult us populations represented in NHANES. J. Nut., Health & Aging, 19, 284-290 (2015).  https://doi.org/10.1007/s12603-014-0569-2
  7. O'Neil, C. E., Fulgoni, V. L., and Nicklas, T. A., Tree Nut consumption is associated with better adiposity measures and cardiovascular and metabolic syndrome health risk factors in US Adults: NHANES 2005-2010. Nutr. J., 14, 1-8 (2005). 
  8. US Food and Drug Administration, (2023, June 1). Qualified health claims: Letter of enforcement discretion-nuts and coronary heart disease. Retrieved from http://wayback.archive-it.org/7993/20171114183724/https://www.fda.gov/Food/IngredientsPackagingLabeling/LabelingNutrition/ucm072926.htm 
  9. Brzezinski A., Melatonin in humans. N. Engl. J. Med., 336, 186-195 (1997).  https://doi.org/10.1056/NEJM199701163360306
  10. Reiter, R.J., Tan, D.X., Mayo, J.C., Sainz, R.M., Leon, J., Czarnocki, Z., Melatonin as an antioxidant: biochemical mechanisms and pathophysiological implications in humans. Acta Biochim. Pol., 50, 1129-1146 (2003).  https://doi.org/10.18388/abp.2003_3637
  11. Posadzki, P.P., Bajpai, R., Kyaw, B.M., Roberts, N.J., Brzezinski, A., Christopoulos, G.I., Divakar, U., Bajpai, S., Soljak, M., Dunleavy, G., Jarbrink, K., Nang, E.E.K., Soh, C.K., Car, J., Melatonin and health: an umbrella review of health outcomes and biological mechanisms of action. BMC Med., 16, 18 (2018). 
  12. Sack, R.L., Lewy, A.J., Erb, D.L., Vollmer, W.M., Singer, C.M., Human melatonin production decreases with age. J. Pineal Res., 3, 379-388 (1986).  https://doi.org/10.1111/j.1600-079X.1986.tb00760.x
  13. Arnao, M.B., Hernandez-Ruiz, J., The potential of phytomelatonin as a nutraceutical. Molecules., 23, 238 (2018). 
  14. Fan, J., Xie, Y., Zhang, Z., Chen, L., Melatonin: a multifunctional factor in plants. Int. J. Mol. Sci., 19, 1528 (2018). 
  15. Arnao, M.B., Hernandez-Ruiz, J., Functions of melatonin in plants: a review. J. Pineal Res., 59, 133-150 (2015).  https://doi.org/10.1111/jpi.12253
  16. Paredes, S.D., Korkmaz, A., Manchester, L.C., Tan, D.X., Reiter, R.J., Phytomelatonin: A review. J. Exp. Bot., 60, 57-69 (2009).  https://doi.org/10.1093/jxb/ern284
  17. Afreen, F., Zobayed, S.M.A., Kozai, T., Melatonin in glycyrrhiza uralensis: Response of plant roots to spectral quality of light and UV-B radiation. J. Pineal Res., 41, 108-115 (2006).  https://doi.org/10.1111/j.1600-079X.2006.00337.x
  18. Kocada˘gli, T., Yilmaz, C., Gokmen, V., Determination of melatonin and its isomer in foods by liquid chromatography tandem mass spectrometry. Food Chem., 153, 151-156 (2014).  https://doi.org/10.1016/j.foodchem.2013.12.036
  19. Bligh, E.G., Dyer, W.J., A rapid method of total lipid extraction and purification. Can. J. Biochem. Physiol., 37, 911-917 (1959).  https://doi.org/10.1139/y59-099
  20. Luzia, D.M.M, Jorge, N., Bioactive substance contents and antioxidant capacity of the lipid fraction of Annona crassiflora Mart. seeds. Ind. Crops Prod., 42, 231-235 (2013).  https://doi.org/10.1016/j.indcrop.2012.05.027
  21. Kates, M., 1986, Techniques of lipidology: isolation, analysis and identification of lipids, 3rd revised ed, Newport-Somerville, Ottawa, Canada, pp. 25-28. 
  22. Reiter, R.J., Tan, D.X., Manchester, L.C., Simopoulos, A.P., Maldonado, M.D., Flores, L.J., Terron, M.P., Melatonin in edible plants (phytomelatonin): identification, concentrations, bioavailability and proposed functions. World Rev. Nutr. Diet., 97, 211-230 (2007). 
  23. K'olar, J., Machackova, I., Illnerova, H., Prinsen, E., Van Dongen, W., Van Onckelen, H.A., Melatonin in higher plant determined by radioimmunoassay and liquid chromatography-mass spectrometry. Biol. Rhythm Res., 26, 406-409 (1995). 
  24. Nawaz, M.A., Huang, Y., Bie, Z., Ahmed, W., Reiter, R.J., Niu, M., Hameed, S., Melatonin: current status and future perspectives in plant science. Front. Plant Sci., 6, 1230-1242 (2016). 
  25. Pranil, T., Moongngarm, A., Loypimai, P., Influence of pH, temperature, and light on the stability of melatonin in aqueous solutions and fruit juices. Heliyon, 6, e03648 (2020). 
  26. Kocada˘gli, T., Yilmaz, C., Gokmen, V., Determination of melatonin and its isomer in foods by liquid chromatography tandem mass spectrometry. Food Chem., 153, 151-156 (2014).  https://doi.org/10.1016/j.foodchem.2013.12.036
  27. Tapia, M.I., Sanchez-Morgado, J.R., Garci-Parra, J., Ramiez, R., Hernandez, T., Gonzalez-Gomez, D., Comparative study of the nutritional and bioactive compounds content of four walnut (Juglans regia L.) cultivars. J. Food Compos. Anal., 31, 232-237 (2013).  https://doi.org/10.1016/j.jfca.2013.06.004
  28. Manchester, L.C., Tan, D.X., Reiter, R.J., Park, W., Monis, K., Qi, W., High levels of melatonin in the seeds of edible plants: possible function in germ tissue protection. Life Sci., 67, 3023-3029 (2000).  https://doi.org/10.1016/S0024-3205(00)00896-1
  29. Meng, X., Li, Y., Li, S., Zhou, Y., Gan, R.Y., Xu, D.P., Li, H.B., Dietary sources and bioactivities of melatonin. Nutrients., 9, 367 (2017). 
  30. Oladi, E., Mohamadi, M., Shamspur, T., Mostafavi, A., Spectrofluorimetric determination of melatonin in kernels of four different Pistacia varieties after ultrasound-assisted solid-liquid extraction. Spectrochim. Acta A. Mol. Biomol. Spectrosc., 132, 326-329 (2014).  https://doi.org/10.1016/j.saa.2014.05.010
  31. Paroni, R., Dei Cas, M., Rizzo, J., Ghidoni, R., Montagna, M.T., Rubino, F.M., Iriti, M., Bioactive phytochemicals of tree nuts. Determination of the melatonin and sphingolipid content in almonds and pistachios. J. Food Compos. Anal., 82, 103227 (2019). 
  32. Balabusta, M., Szafranska, K., Posmyk, M.M., Exogenous melatonin improves antioxidant defense in cucumber seeds (Cucumis sativus L.) germinated under chilling stress. Front. Plant Sci., 7, 575 (2016). 
  33. Ortiz, C.M., Vicente, A.R., Fields, R.P., Grillo, F., Labavitch, J.M., Donis-Gonzalez, I., Crisosto, C.H., Walnut (Juglans regia L.) kernel postharvest deterioration as affected by pellicle integrity, cultivar and oxygen concentration. Postharvest Biol. Technol., 156, 110948 (2019).