식물병연구 (Research in Plant Disease)

한국식물병리학회 (The Korean Society of Plant Pathology)
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식물 유용 방선균 2종의 배양 및 포자생성 최적화 조건 탐색

Optimization of Culture and Sporulation for Two Plant Beneficial Streptomyces Strains

  • 김다란 (경상국립대학교 생명과학연구원) ;
  • 곽연식 (경상국립대학교 생명과학연구원)
  • Da-Ran Kim (Resarch Institute of Life Science, Gyeongsang National University) ;
  • Youn-Sig Kwak (Resarch Institute of Life Science, Gyeongsang National University)
  • 투고 : 2023.04.24
  • 심사 : 2023.05.20
  • 발행 : 2023.06.30
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초록

관행적 화학농약의 식물병 관리 및 치료의 한계적인 효과는 유익한 세균이나 미생물을 이용한 식물병 제어 방법 개발의 필요성을 나타낸다. 지속 가능한 농업은 특히 다양한 항생물질과 이차 대사 생성물을 생산하는 방선균의 농업적 중요성을 시사하며, Streptomyces globiosporus SP6C4균주와 Streptomyces sp. S8은 강력한 항균 작용을 가지고 있으며, 지속가능한 농업에서 작물 생장을 개선하기 위한 우수한 균주로 인정되고 있다. 본 연구에서는 다양한 질소원과 탄소원을 활용하여 대상 미생물 Streptomyces 두 균주의 생장을 촉진시키는 방법을 조사하였다. L-글루타민과 L-시스테인이 첨가된 조건에서 S. globiosporus SP6C4와 Streptomyces sp. S8 균주의 포자 생성 능력이 증가하였으며, 각 균주의 생장이 촉진되었다. 이러한 결과는 유용 미생물의 대량배양 기술의 범위 확장과 농업미생물의 실용화에 기여할 것으로 생각된다.

The limited effectiveness of current plant disease management treatments necessitates the development of new methods for controlling diseases using beneficial microbes. Demanding sustainable agriculture is increasingly highlighted as a biocontrol approach, particularly Streptomyces species known to produce a variety of antibiotic compounds and secondary metabolites. The Streptomyces globisporus SP6C4 strain and Streptomyces sp. S8 have been reported as potent antifungal agents and are gaining attention for improving crop growth in sustainable agriculture. In this study, we investigated the use of Streptomyces species formulations to enhance bacterial growth with nitrogen sources. Specifically, the addition of L-glutamic acid and L-cysteine resulted in earlier sporulation and bacterial growth in Streptomyces strains, respectively. This approach could expand the range of fermentation techniques in agriculture and be useful for controlling plant growth-promoting bacteria.

키워드

과제정보

This research was supported by an agenda research program by the Rural Development Administration (PJ015871).

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