Natural Product Sciences

The Korean Society of Pharmacognosy (한국생약학회)
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Optimization of the Extraction of Polyphenols and Flavonoids from Argania spinosa Leaves using Response Surface Methodology

  • Rajaa Moundib (Microbiology, Health and Environment Team, Faculty of Sciences, Chouaib Doukkali University) ;
  • Hamadou Sita (Microbiology, Health and Environment Team, Faculty of Sciences, Chouaib Doukkali University) ;
  • Ismail Guenaou (Health and Environment Laboratory, Ain Chock Faculty of Sciences, Hassan II University of Casablanca ) ;
  • Fouzia Hmimid (Health and Environment Laboratory, Ain Chock Faculty of Sciences, Hassan II University of Casablanca )
  • Received : 2023.03.23
  • Accepted : 2023.04.26
  • Published : 2023.06.30
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Abstract

To our knowledge, this is the first study aiming to optimize the extraction conditions of total phenolic compounds (TPC) and total flavonoids contents (TFC) from Argania spinosa leaves using Response Surface Methodology (RSM) with a Box-Behnken design (BBD). The optimal conditions obtained were 5% (w/v) solvent-to-solid ratio, 72.33% ethanol concentration, and 10h ours as an extraction time, which resulted in an extract with maximum TPC (131.63 mg GAE/g dw) and TFC (10.66 mg QE/g dw). Under the optimal extraction conditions, the antioxidant activity of the extracts of leaves of argan tree showed a moderate antiradical capacity of DPPH (IC50 = 0,130 mg/mL) and ABTS (IC50 = 0.198 mg/mL). However, the leaves of argan tree showed a very interesting reducing power of Iron (IC50 = 0.448 mg/ml) which is similar to that of the ascorbic acid (IC50 = 0.371 mg/mL).

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References

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