한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제51권2호
  • /
  • Pages.174-183
  • /
  • 2023
  • /
  • 1598-642X(pISSN)
  • /
  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지
DOI QR코드

DOI QR Code

Antioxidant and Antibacterial Effects of Mixed Extracts of Phyllanthus emblica, Geranium (Pelargonium graveolens) and Commiphora myrrha: Possibility of Natural Materials for Acne Treatment

  • Mi Jeong Choi (Biomedical Biotechnology Research Institute Co., Ltd.) ;
  • Yu Ri Kim (Biomedical Biotechnology Research Institute Co., Ltd.)
  • 투고 : 2022.10.19
  • 심사 : 2023.06.15
  • 발행 : 2023.06.28
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Various skin diseases are occurring due to external factors such as urbanization and increase in environmental pollution and wearing masks due to COVID-19. Accordingly, various functional cosmetics are being released, but as some side effects are reported, research on functional cosmetics materials using natural plants is necessary. Therefore, in this study, the antioxidant, antibacterial and anti-inflammatory effects of Phyllanthus emblica, Geranium (Pelargonium graveolens), and Commiphora myrrha mixed extracts (PGC) that pharmacological efficacy has been verified were analyzed and their potential as functional cosmetics raw materials was examined. Four extracts (PGC-1~4) were prepared according to the extraction method. ABTS and DPPH radical scavenging activity experiments were conducted for the antioxidant efficacy of the extracts. In addition, paper disc experiments and LPS inflammation-inducing cytokine experiments were conducted to examine the antibacterial and anti-inflammatory effects. In addition, a cell viability test was performed to confirm cytotoxicity. As results of the study, all extracts showed antioxidant, antibacterial, and anti-inflammatory effects without cytotoxicity, and in particular, PGC-4, a fermentation and ultrasonic extract, showed the best efficacy. This means that the extraction yield of useful components varies depending on the extraction method.

키워드

참고문헌

  1. Bae CH, Cho WY, Kim HJ, Ha OK. 2020. An experimental comparison of cnn-based deep learning algorithms for recognition of beauty-related skin disease. J. Korea Soc. Comput. Info. 25: 25-34. 
  2. Pande G, Akoh CC. 2010. Organic acids, antioxidant capacity, phenolic content and lipid characterisation of Georgia-grown underutilized fruit crops. Food Chem. 120: 1067-1075.  https://doi.org/10.1016/j.foodchem.2009.11.054
  3. Dasaroju S, Gottumukkala KM. 2014. Current trends in the research of Emblica officinalis (Amla): A pharmacological perspective. Int. J. Pharm. Sci. Rev. Res. 24: 150-159. 
  4. Kim SC, Ahn KJ, Hann SK, Kim JW, Sung KJ, Kye YC, et al. 2003. Clinico-epidemiologic study on the abuse, misuse, and adverse effects of topical dermatologic drugs. Korean J. Dermatol. 41: 1129-1135. 
  5. Zaenglein AL. 2018. Acne vulgaris. New Eng. J. Med. 379: 1343-1352.  https://doi.org/10.1056/NEJMcp1702493
  6. Webster GF. 1995. Acne vulgaris: state of the science. Arch. Dermatol. 135: 1101-1102.  https://doi.org/10.1001/archderm.135.9.1101
  7. Brown SK, Shalita AR. 1998. Acne vulgaris. Lancet 351: 1871-1876.  https://doi.org/10.1016/S0140-6736(98)01046-0
  8. Weon JB, Ahn JH, Ma CJ. 2011. Antibacterial activity of some medicinal plants against Propionibacterium acnes. Korean J. Pharmacog. 42: 98-101. 
  9. Lee NH, Choi EH, Ahn SK, Lee SH. 1998. A clinical study on the effect of a facial cleanser consisting of 1% triclosan and 0.5% Ku Shen on acne vulgaris. Korean J. Dermatol. 36: 871-876. 
  10. Leem MH, Jung EH. 2005. Changes of pH, sebum, moisture excreation level of skin surface after benzoyl peroxide lotion treatment on acne skin. Korean J. Aesthetics Cosmetol. 3: 37-48. 
  11. Bojar RA, Holland KT, Cunliffe WJ. 1991. The in-vitro anti-microbial effects of azelaic acid upon Propionibacterium acnes strain P37. J. Antimicrob. Chemother. 28: 843-853.  https://doi.org/10.1093/jac/28.6.843
  12. Aires JR, Pechere JC, Van Delden C, Kohler T. 2002. Amino acid residues essential for function of the MexF efflux pump protein of Pseudomonas aeruginosa. Antimicrob. Agents Chemother. 46: 2169-2173.  https://doi.org/10.1128/AAC.46.7.2169-2173.2002
  13. Leyden JJ, Kaidbey K, Gans EH. 1996. The antimicrobial effects in vivo of minocycline, doxycycline and tetracycline in humans. J. Dermatol. Treatment 7: 223-225.  https://doi.org/10.3109/09546639609089553
  14. Lim YH, Kim IH, Seo JJ. 2007. In vitro activity of kaempferol isolated from the impatiens balsamina alone and in combination with erythromycin or clindamycin against Propionibacterium acnes. J. Microbiol. 45: 473-477. 
  15. Kobayashi M, Kabashima K, Nakamura M, Tokura Y. 2009. Effects of oral antibiotic roxithromycin on quality of life in acne patients. J. Dermatol. 36: 383-391.  https://doi.org/10.1111/j.1346-8138.2009.00664.x
  16. Dasaroju S, Gottumukkala KM. 2014. Current trends in the research of Emblica officinalis (CP): A pharmacological perspective. Int. J. Pharm. Sci. Rev. Res. 24: 150-159. 
  17. Tsuji N, Moriwaki S, Suzuki Y, Takema Y, Imokawa G. 2001. The role of elastases secreted by fibroblasts in wrinkle formation: implication through selective inhibition of elastase activity. Photochem. Photobiol. 74: 283-290.  https://doi.org/10.1562/0031-8655(2001)074<0283:TROESB>2.0.CO;2
  18. Gates MA, Tworoger SS, Hecht JL, De vivo I, Rosner B, Hankinson SE. 2007. A prospective study of dietary flavonoid intake and incidence of epithelial ovarian cancer. Int. J. Cancer 121: 2225-2232.  https://doi.org/10.1002/ijc.22790
  19. Akdemir ZS, Tatli II, Saracoglu I, Ismailoglu U, Calis I, Inci SE. 2001. Polyphenolic compound from Geranium pratense and their free radical scavenging activities. Phytochemistry 56: 189-193.  https://doi.org/10.1016/S0031-9422(00)00367-8
  20. Prabuseenivasan S, Jayakumar M, Ignacimuthu S. 2006. In vitro antibacterial activity of some plant essential oils. BMC Complement. Altern. Med. 6: 39. 
  21. Su S, Wang T, Duan JA, Zhou W, Hua YQ, Tang YP, et al. 2011. Anti-inflammatory and analgesic activity of different extracts of Commiphora myrrha. J. Ethnopharmacol. 134: 251-258.  https://doi.org/10.1016/j.jep.2010.12.003
  22. Sotoudeh R, Gholamnezhad Z, Aghaei A. 2019. The anti-diabetic and antioxidant effects of a combination of Commiphora mukul, Commiphora myrrha and Terminalia chebula in diabetic rats. Avicenna J. Phytomed. 9: 454-464. 
  23. Madia VN, De Angelis M, De Vita D, Messore A, De Leo A, Ialongo D, et al. 2021. Investigation of Commiphora myrrha (Nees) Engl. oil and its main components for antiviral activity. Pharmaceuticals 14: 243-254.  https://doi.org/10.3390/ph14030243
  24. Que F, Mao L, Pan X. 2006. Antioxidant activities of five Chinese rice wines and the involvement of phenolic compounds. Food Res. Int. 39: 581-587.  https://doi.org/10.1016/j.foodres.2005.12.001
  25. Arnao MB. 2000. Some methodological problems in the determination of antioxidant activity using chromogen radicals: a practical case. Trends Food Sci. Technol. 11: 419-421.  https://doi.org/10.1016/S0924-2244(01)00027-9
  26. Jeong JA, Kwon SH, Lee CH. 2007. Screening for anti-oxidative activities of extracts from aerial and underground parts of some edible and medicinal ferns. Korean J. Plant Resour. 20: 185-192. 
  27. Aoshima H, Tsunoue H, Koda H, Kiso Y. 2004. Aging of whiskey increases 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity. J. Agric. Food Chem. 52: 5240-5244.  https://doi.org/10.1021/jf049817s
  28. Kim MK. 2021. Investigation of antimicrobial activity of rutaceae fruit ethanol extracts against microorganisms-induced skin inflammation. J. Korea Soc. Comput. Information 26: 237-245. 
  29. Wang SJ, Lee HJ, Cho JY, Park KH, Moon JH. 2012. Isolation and identification of antioxidants from makgeolli. Korean J. Food Sci. Technol. 44: 14-20.  https://doi.org/10.9721/KJFST.2012.44.1.014
  30. Guzik TJ, Korbut R, Adamek-Guzik T. 2003. Nitric oxide and superoxide in inflammation and immune regulation. J. Physiol. Pharmacol. 43: 469-487. 
  31. Graca VC, Ferreira IC, Santos PF. 2016. Phytochemical composition and biological activities of Geranium robertianum L.: A review. Ind. Crops Prod. 87: 363-378.  https://doi.org/10.1016/j.indcrop.2016.04.058
  32. Hamed AM, Awad AA, Abdel-Mobdy AE, Alzahrani A, Salamatullah AM. 2021. Buffalo yogurt fortified with Eucalyptus (Eucalyptus camaldulensis) and Myrrh (Commiphora Myrrha) essential oils: New insights into the functional properties and extended shelf life. Molecules 26: 6853. 
  33. Fu LW, Vender RB. 2011. Newer approaches in topical combination therapy for acne. Skin Ther. Lett. 16: 3-6. 
  34. Koreck A, Pivarcsi A, Dobozy A, Kemeny L. 2003. The role of innate immunity in the pathogenesis of acne. Dermatology 206: 96-105.  https://doi.org/10.1159/000068476
  35. Witold M, Aleksander K. 2003. Preliminary assessment of alginic acid as a factor buffering triethanol amine interacting with artificial skin sebum. Eur. J. Pharm. Biopharm. 55: 237-240.  https://doi.org/10.1016/S0939-6411(02)00195-9
  36. Kim N, Lim YH, Park SW, Nam ES. 2009. Antimicrobial activities of the anti-acne compounds from natural sources. Microbiol. Biotechnol. Lett. 37: 80-84. 
  37. Lee JY, Son HJ. 2018. Trends in the efficacy and safety of ingredients in acne skin treatments. Asian J. Beauty Cosmetol. 16: 449-463.  https://doi.org/10.20402/ajbc.2017.0199
  38. Chan GY, Kim HJ. 2011. Antimicrobial effects of extracts of Taraxacum officinale H. on acnes strains. Int. J. Integrated CAM 7: 3-16. 
  39. Laksmitawati DR, Prasanti AP, Larasinta N, Syauta GA, Hilda R, Ramadaniati HU, et al. 2016. Anti-inflammatory potential of gandarusa (Gendarussa vulgaris Nees) and soursoup (Annona muricata L) extracts in LPS stimulated-macrophage cell (RAW264. 7). J. Nat. Remedies 16: 73-81.  https://doi.org/10.18311/jnr/2016/5367
  40. Kim SH, Kang SA. 2019. Anti-inflammatory effects of beopje processed curly dock (Rumex crispus L.) in LPS- induced murine RAW 264.7 cell lines. Korean J. Food Nutr. 32: 408-416. 
  41. Jang GY, Kim HY, Lee SH, Kang YR, Hwang IG, Woo KS, et al. 2012. Effects of heat treatment and extraction method on antioxidant activity of several medicinal plants. J. Korean Soc. Food Sci. Nutr. 41: 914-920.  https://doi.org/10.3746/jkfn.2012.41.7.914
  42. Chemat F, Khan MK. 2011. Applications of ultrasound in food technology: processing, preservation and extraction. Ultrason. Sonochem. 18: 813-835.  https://doi.org/10.1016/j.ultsonch.2010.11.023
  43. Kim HY, Choi DH, Kang DG, Lee HS. 2009. Effects of Oenothera odorata on vasorelaxation in thoracic and carotid artery. FASEB Exp. Biol. J. 23: 939-18. 
  44. Kim JH, Lee SH. 2016. Antioxidative and antimicrobial activities of Oenothera biennis extracted by different methods. Korean J. Food Preserv. 23: 233-238.  https://doi.org/10.11002/kjfp.2016.23.2.233
  45. Klein A, He X, Roche M, Mallett A, Duska L, Supko JG, et al. 2006. Prolonged stabilization of platinum-resistant ovarian cancer in a single patient consuming a fermented soy therapy. Gynecol. Oncol. 100: 205-209.  https://doi.org/10.1016/j.ygyno.2005.08.006
  46. Park MR, Yoo C, Chang YN, Ahn BY. 2012. Change of total polyphenol content of fermented Gastrodia elata blume and radical scavenging. Korean J. Plant Resour. 25: 379-386. https://doi.org/10.7732/kjpr.2012.25.4.379