Measuring the effects of estrus on rumen temperature and environment, behavior and physiological attributes in Korean Native breeding cattle

Abstract

In this study, rumen temperature and environment in estral and non-estral Korean Native breeding cattle were evaluated by using a bolus sensor. Behavioral and physiological changes in study animals were also assessed. To assess the rumen temperature and environment, we inserted bolus sensors into 12 Korean Native cattle with an average age of 35.5 months, then measured temperature and activity within the rumen using the wireless bolus sensor. Drinking, feeding and mounting behavior, and measured vaginal temperature and levels of intravaginal mucus resistance were recorded. We found that cattle in estrus exhibited more acts of mounting (37.4 vs. 0 times/day), increased vaginal temperature (39.0℃ vs. 38.4℃), and decreased vaginal mucus resistance (136.3 Ω vs 197.4 Ω), compared with non-estral animals. Furthermore, increased levels of rumen activity were most significant in estrus cattle at the highest activity levels (p < 0.01). Overall, the estrus group exhibited increased rumen temperature (p = 0.01), compared with the non-estrus group. In conclusion, the results of this study not only provide basic physiological data related to estrus in improved Korean Native breeding cattle, but also suggest that monitoring of rumen temperature and activity might be used as an effective smart device for estrus detection.

INTRODUCTION

For farmers who raise Korean Native breeding cattle, the optimal time of insemination is a critically important matter because calf production directly affects farm income. The best way to predict the timing of fertilization is to observe the estrus behavior and condition of cattle. In South Korea, as elsewhere, visual observations reveal that cattle in estrus typically mount other animals, or allow themselves to be mounted. Korean studies found that that 98.3% of cattle in estrus allowed themselves to be mounted, and 74.6% mounted other animals [1,2]. Other indicators of estrus include mucus secretion from the vagina, changes in vulval color, anxiety, and excitement [3,4]. However, studies of behavioral, physiological, and endocrine changes during estrus have not yet considered fully the particular breeding environment associated with Korean beef cattle [5–7].

In small farm settings, estrus indicators are usually easy to identify visually. On larger farms, it can be difficult to determine optimum insemination times because there is less time available for visual observation of individual animals. However, new developments in information and communications technology (ICT) such as bolus sensors may help to overcome such human resource limitations. Recently, researchers have monitored the rumen environment in exotic species using a rumen-inserted bolus sensor in order to identify estrus [8–11]. The estrus state can also be confirmed by behavioral observations [3,4]. Several studies have reported behavioral changes due to estrus including changes in standing time, mounting behavior, activity levels, and rumen movement associated with action of the nervous system and endocrine system [12–14]. Researchers have also recorded increased rumen temperature during estrus in exotic breeding cattle [11,15]. However, the use of ICT for estrus verification in Korean Native cattle has not been attempted to date. Therefore, in this study, we sought to investigate changes in behavior, physiology, and endocrine indicators during estrus in recently genetically improved Korean Native animals, and to interpret estrusrelated changes in rumen temperature and activity automatically recorded by rumen-inserted bolus sensors.

MATERIALS AND METHODS

Animals and diet

The experimental procedure and methods were approved by the Animal Welfare and Ethics Authority of Konkuk University, Seoul, Korea (KU22106).

For our study, 12 healthy Korean Native breeding cattle aged 38.8 months with fertility of 2.67 ± 1.37 were used. This study was carried out at the Farm at Chungcheongnam-do in Korea, from September to November, 2021. All animals in a roofed feedlot area with open sides were housed. Feed consisted of commercial cattle concentrate and rice straw, with free access to water (Table 1). Feed was given twice daily (07:00 and 17:00).

Table 1. Chemical compositions of concentrates and rice straw used in this study

¹⁾The chemical compositions of concentration were provided by Nonghyup feed, Inc (Seoul, South Korea).

²⁾The chemical compositions of rice straw were obtained from the farming portal of the Rural Development Administration. DM, dry matter.

The wireless bolus sensor

Bolus wireless sensor probes (Dongbang S&D, Seoul, Korea) which are disposable, cylindershaped devices suitable for oral administration to cattle were acquired. The probe detects and communicates rumen temperature and activity by means of a temperature sensor, a three-axis accelerometer (STMicroelectroincs, Geneva, Switzerland), a transmitter (Dongbang S&D) and a battery (Sensirion AG, Staefa, Switzerland). The probe diameter is 3.2 cm, its length is 10.8 cm, and its weight is 180 g. The probe is administered orally via a bolus gun (Leedstone, Melrose, MN, USA) into the rumen. Transmissions are in the 900 MHz band. Average current consumption is less than 100 uA. In our study, data on rumen temperature and activity were transmitted at tenminute intervals from the temperature sensor and three-axis accelerometer, respectively. A receiver which stored all data on a cloud-connected server and could be remotely monitored via a website (http://dongbangsnd.ml/) was used. The distance from the probe to the receiver was about 30 m.

Behavior, physiology, endocrine indicators, rumen temperature and activity in estrus and non-estrus cattle

CCTV (IDIS, Daejeon, Korea) was used to acquire data on animal behavior during estrus and non-estrus periods. We gathered data concerning mounting (other animals), drinking, and feeding behavior for a non-estrus period of 3 days before estrus from 10 to 12 days, and then determined average values for the 12 heads. Estrus animal data covered a 24-hour period consisting of 12 hours prior to the confirmation of estrus, and the 12 hours following. Then, an average value of 16 periods of estrus from 12 heads of cattle was determined. Mounting behavior as the number of attempts made to mount another animal was measured. We assessed water intake by the number of occasions on which water was consumed.

Specialist equipment to collect data concerning vaginal temperature (MT1681, Microlite, Germany) and electrical resistance of vaginal mucus (EDC2, Draminski, Poland) were used, on average, we carried out these measurements once for estrus cattle and three times for non-estrus animals. Vaginal temperatures by inserting the temperature-measuring part of the thermometer into the animal’s vagina for 1 minute were measured. To measure the electrical resistance of vaginal mucus, we inserted the measuring device up to the entrance of the cervical canal. Then, rotated the device three times in the same direction, and recorded the value obtained.

To measure luteinizing hormone, blood from animals on two occasions was collected. Blood was collected from the jugular vein 10 to 12 days before the expected onset of estrus, then took blood again within 3 hours of estrus detection. The collected blood was immediately centrifuged at 2,700×g, 4℃, transferred to a 1.5-mL tube, and stored at −80℃. Later, after thawing, concentrations of luteinizing hormone in serum were analyzed by measuring absorbance at 450 nm using a microplate reader (PMT49984, BioTek Instruments, Winooksi, VT, USA) and ELISA kit (EK760147, AFG Scientific, Shizuoka, Japan).

Data on temperature and activity in the rumen were obtained by using the bolus sensor. We first collected data 10 to 12 days before the expected onset of estrus. As this time approached, the experimenter and a veterinarian visually checked for signs of estrus every 4 hours. After estrus was confirmed, data for the 12 hours before and after confirmation time were used. The onset of estrus by measuring vaginal temperature and electrical resistance of vaginal mucus was also confirmed. We expressed our rumen temperature data in terms of means, minimum values, maximum values, proportions of animals with below-average and above-average temperatures, as well as proportions of animals with temperatures exceeding 39.5℃. For rumen activity, we determined average, minimum and maximum activity levels, and classified activities recorded in terms of numbered levels rising from zero to eight or above.

Statistical analysis

Data of luteinizing hormone, behavioral activity and physiological changes were analyzed, as well as activity data obtained from the ruminal sensor, by means of repeated measures analysis using SAS version 9.4 (SAS Institute, Cary, NC, USA). Our model was as follows:

Y_ijk = μ +α_i + β_j + γ(α)_ik + ε_ijk

where Y_ijk is the observation of breeding cattle k at sampling time j with or without estrus i, μ is the overall mean, α_i is the fixed effect of treatment i (non-estrus and estrus), β_j is the fixed effect of sampling time j, γ(α)_ik is the random effect of breeding cattle k nested in treatment i and ε_ijk is the residual effect. We included calf identity in the model as a random effect. Covariance structures (autoregressive order 1, compound symmetry, unstructured, and variance components) for the repeated measures model were tested and chose the structure which best fitted the model based on the lowest value of the Schwarz–Bayesian information criterion. Data were present as least square means and associated standard error of means. We compared least square means among treatments using Student’s t-test comparison when the treatment effect was tendency or significant. We considered differences to be statistically significant if the p-value was lower than 0.05. Means with p-values between 0.05 and 0.10 reflected a tendency to differ.

RESULTS AND DISCUSSION

Our data on the concentration of serum luteinizing hormone revealed that hormone concentration increased from 22.79 mIU/mL in non-estrus cattle to 38.38 mIU/mL in estrus animals, i.e., an increase of 15.59 mIU/mL (p < 0.01) (Table 2). Estrus involves cyclical changes in levels of various hormones; the most representative of these are progesterone and luteinizing hormone. In dairy cows, the authors of [16] found that progesterone, a hormone secreted from the corpus luteum, was maintained at a low level of less than 1 ng/mL, then rose to a peak level of 4.50 ng/mL on subsequent days 15 to 17 and then decreased rapidly before estrus. Contrarily, Snook and coworkers [17] found that luteinizing hormone maintained an average level of 5.1 ng/mL in non-estrus cattle but increased to 20.7 ng/mL on the day of estrus [17]. Studies by Henricks et al. [18] and Chenault et al. [19] found that increases in luteinizing hormone varied across studies. The data in our study revealed an increase in concentration of serum luteinizing hormone on the day of estrus.

Table 2. Comparison of LH levels, mounting behavior, drinking behavior, vaginal temperature and electrical resistance of vaginal mucus between non-estrus and estrus cattle​​​​​​​

¹⁾Statistical analysis carried out using Student’s t-test.

²⁾Luteinizing hormone level of non-estrus shows the average value of 12 heads. Estrus data is an averaged value of 16 estrus periods of 12 heads.

³⁾Non-estrus animal data collected for 3 days before estrus from 10 to 12 days is an averaged value of 12 heads. Estrus data obtained for 12 hours prior to estrus confirmation and for 12 hours following is an averaged value of 16 periods of estrus of 12 heads.

LH, luteinizing hormone.

Concerning mounting behavior, our CCTV observations showed that mounting was not attempted when cattle were not in estrus. However, when in estrus, animals performed mounting behaviors on an average of 37.4 occasions (Table 2). Cattle in estrus typically mount other animals or allow themselves to be mounted. Previous studies have found that, during estrus, 98.3% of cattle mount other animals, and 74.6% allow themselves to be mounted [1,2]. One study found that cattle performed an average of 7.6 mounting behaviors over a period of approximately 8 to 9 hours [20,21]. Factors affecting the number of mountings include external temperature, the physical properties of the breeding area, and the number of animals raised together [22]. The study of Sveberg et al. [21] involved twenty-two breeding animals in a space of 17 × 24 m. In our study, twelve animals were housed in an area of 12.5 × 25 m. When considering average numbers of mounting behaviors, the nature of the breeding environment should be considered when carrying out comparisons between studies, as well as the species involved.

We also found increased water intake during estrus, from 3.1 to 3.6 drinking occasions, on average (p < 0.01) (Table 2). One previous study [23] reported a lower frequency of water intake of cattle during estrus, due to a decrease in the intake of roughage without compensating changes in the intake of concentrate feed. The authors of [24] found that water intake fell from an average of four occasions in non-estrus cattle to an average of three in estrus animals. In addition, the duration of water visits also decreased during estrus. In our study, though we did not measure the volume of water consumed, we did find an increase in water-drinking occasions in estrus animals, as stated above, and this might be associated with increased cattle activity during estrus periods.

Average vaginal temperature in our cattle increased from 38.4℃ in the non-estrus state to 39.0℃ during estrus (p < 0.01) (Table 2). This is in line with studies of another cattle breed [25,26], which found that the average vaginal temperature of Holstein cows increased by between 0.4℃ and 0.6℃, and this increase in vaginal temperature was maintained for 3 to 6.8 hours.

Average level of electrical resistance in the vaginal mucus of cattle in our study was 44.8% lower in estrus than non-estrus animals (136.3 Ω vs. 197.4 Ω) (p < 0.01) (Table 2). Factors affecting the electrical resistance in vaginal mucus include higher amounts of mucus in the vagina due to an increase in estrogen secretion and an increase in ions such as NaCl which leads to lower resistance, as reported by the authors of [27]. Other researchers have measured electrical resistance of vaginal mucus during the estrus cycle and found that it gradually decreases before estrus, remains low during estrus, and returns to its original level after estrus [28,29]. In our study, we observed a similar pattern. The authors of [30] also measured electrical resistance in vaginal mucus of cattle. Although they obtained differing results for different breeds, they still found resistance decreased during estrus, by an average of 40.04%, from 49.7 Ω to 29.8 Ω.

The rumen-inserted bolus sensor used in this study indicated activity levels by means of an acceleration sensor which measured gravitational acceleration every 2.5 seconds for 10 min. We determined all activities recognized by the sensor in the rumen to be 100%. We then found that times of inactivity decreased from 46.25% in non-estrus cattle to 28.76% in estrus animals, i.e., a reduction of 17.49% (Table 3). We thus numerically confirmed that inactivity decreased significantly during estrus. Level ‘1’ activity also significantly decreased during estrus, from 18.94% to 14.39% (p < 0.01). For activity levels ‘2’ to ‘6’, we found no statistically significant differences between the non-estrus and estrus periods (p > 0.05). Activity at the high levels of ‘7’ or above was 10.79% in non-estrus cattle and 27.65% in estrus animals (p < 0.01). These findings contrast with the abovedescribed activity levels ‘zero’ and ‘1’, and confirmed that the activity level increased during estrus. This increase rate was matched by the decrease in the activity level of ‘zero’ (17.49%) and the activity level of ‘7’ or more (16.86%). Previous studies have considered such levels of activity. The authors of [31] found that walking activity increased by 290% on the day of estrus confirmation, compared with 7 days previously, and the authors of [12] found increased standing time prior to ovulation. In line with previous studies, therefore, we found that activity during estrus increased significantly. Using the rumen-inserted bolus sensor, we found that estrus could be confirmed if activity levels ‘zero’ and ‘1’ decreased by 22.0%, while activity levels ‘7’ or higher increased by 16.8%.

Table 3. Comparison of activity levels between non-estrus and estrus cattle based on data obtained using the bolus sensor​​​​​​​

¹⁾Statistical analysis carried out using Student’s t-test.

²⁾Non-estrus animal data collected for 3 days before estrus from 10 to 12 days is an averaged value of 12 heads. Estrus data obtained for 12 hours prior to estrus confirmation and for 12 hours following is an averaged value of 16 periods of estrus of 12 heads.

³⁾Activity levels 0, 1, 2, 3, 4, 5, 6, 7, and ≥ 8 refer to the percentage of total daily activity measurements, as follows: (0≥8 number of activity measurements / total daily activity measurements) × 100.

As shown in Table 4, when we observed rumen temperature, average temperature in non-estrus animals was 38.76℃. During estrus, the corresponding figure was 38.8℃, i.e., a rise of 0.13℃ (p = 0.01). We found no significant difference in minimum rumen temperature between the two​​​​​​​ groups (p > 0.05), while maximum temperature was 39.91℃ in estrus cattle and 39.67℃ in non-estrus animals, i.e., an increase of 0.24℃ (p = 0.03). The rumen-inserted bolus sensor used in this study might have influenced these temperature changes by affecting drinking water intake, which did not show a clear change as did vaginal temperature. However, we did identify a significant difference between estrus and non-estrus animals. Because the average temperature in the rumen for differs between the two groups, we determined a base value for rumen temperature on the 10th to the 12th days before estrus. Subsequently, we checked for values higher or lower than the base temperature. First, we defined measurements of temperature lower than or equal to the base temperature as ‘below-average’ temperature. We defined temperature measurements higher than the base temperature as ‘above-average’ temperature. ‘Below-average’ measurements made up 34.29% of the non-estrus and 28.71% of the estrus data, while ‘above-average’ measurements made up 65.71% of the non-estrus and 71.29% of the estrus data (p = 0.04). Proportions for temperatures beyond 39.5℃ were 2.78% and 15.97% for non-estrus and estrus groups, respectively, i.e., a large difference of 13.19% (p > 0.01). In summary, when considering the variability between individuals, we judged that the use of rumen temperature data could contribute to a more accurate means of estrus detection than visual inspection alone.

Table 4. Comparison of ruminal temperatures between non-estrus and estrus of cattle based on data obtained using the bolus sensor​​​​​​​

¹⁾Statistical analysis carried out using Student’s t-test.

²⁾Non-estrus animal data collected for 3 days before estrus from 10 to 12 days is an averaged value of 12 heads. Estrus data obtained for 12 hours prior to estrus confirmation and for 12 hours following is an averaged value of 16 periods of estrus of 12 heads.

³⁾Based on the average ruminal temperature of non-estrus animals, the data refers to the probability of a number of times below or excess the average temperature.

⁴⁾(Number of times of over 39.5℃ / total daily ruminal temperature measurements) × 100.​​​​​​​

CONCLUSION

In this study, we investigated changes in behavior, physiology, and endocrine indicators during estrus in Korean Native breeding cattle. We interpreted estrus-related changes in rumen temperature and activity automatically recorded by a wireless bolus sensor. Compared with non-estrus cattle, animals in estrus exhibited increases in luteinizing hormone levels, mounting behavior, drinking water intake, and vaginal temperature, as well as decreased electrical resistance of vaginal mucus. Using the rumen-inserted bolus sensor, we also found increased rumen temperature and activity. The results of our study not only provide basic physiological data related to estrus in improved Korean Native breeding cattle, but also suggest that monitoring of rumen temperature and activity can be used as an effective smart device for estrus detection in Korea.