Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Korean Ginseng Berry Polysaccharide Enhances Immunomodulation Activities of Peritoneal Macrophages in Mice with Cyclophosphamide-Induced Immunosuppression

  • JeongUn Choi (Department of Wellness-Bio Industry, Gangneung-Wonju National University) ;
  • Ju Hyun Nam (Department of Wellness-Bio Industry, Gangneung-Wonju National University) ;
  • Weerawan Rod-in (Department of Marine Food Science and Technology, Gangneung-Wonju National University) ;
  • Chaiwat Monmai (Department of Marine Food Science and Technology, Gangneung-Wonju National University) ;
  • A-yeong Jang (Department of Wellness-Bio Industry, Gangneung-Wonju National University) ;
  • SangGuan You (Department of Wellness-Bio Industry, Gangneung-Wonju National University) ;
  • Woo Jung Park (Department of Wellness-Bio Industry, Gangneung-Wonju National University)
  • Received : 2022.11.28
  • Accepted : 2023.03.21
  • Published : 2023.06.28
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Abstract

Korean ginseng (Panax ginseng C. A. Meyer), a member of the Araliaceae family, is known as a traditional medicinal plant to have a wide range of health properties. Polysaccharides constitute a major component of Korean ginseng, and its berries exhibit immune-modulating properties. The purpose of this study was to investigate the immune effects of crude polysaccharide (GBPC) extracted from Korean ginseng berry on peritoneal macrophages in mice with cyclophosphamide (CY)- induced immunosuppression. BALB/c mice were divided into eight groups: normal control, normal control + CY, levamisole + CY, ginseng + CY, and four concentrations of 50, 100, 250, and 500 mg/kg BW/day of GBPC + CY. Mice were orally administered with samples for 10 days. Immunosuppression was established by treating mice with CY (80 mg/kg BW/day) through intraperitoneal injection on days 4 to 6. The immune function of peritoneal macrophages was then evaluated. Oral administration of 500 mg/kg BW/day GBPC resulted in proliferation, NO production, and phagocytosis at 100%, 88%, and 91%, respectively, close to the levels of the normal group (100%) of peritoneal macrophages. In CY-treated mice, GBPC of 50-500 mg/kg BW/day also dose-dependently stimulated the proliferation, NO production, and phagocytosis at 56-100%, 47-88%, and 53-91%, respectively, with expression levels of immune-associated genes, such as iNOS, COX-2, IL-1β, IL-6, and TNF-α, of about 0.32 to 2.87-fold, compared to those in the CY group. GBPC could be a potential immunomodulatory material to control peritoneal macrophages under an immunosuppressive condition.

Keywords

Acknowledgement

This research project was supported by the University Emphasis Research Institute Support Program (No. 2018R1A61A03023584), funded by the National Research Foundation of Korea. This research was also supported by Korea Institute of Marine Science & Technology Promotion (KIMST) funded by the Ministry of Oceans and Fisheries (20220042, Korea Sea Grant Program: GangWon Sea Grant).

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