Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Alpha-Glucosidase Inhibitory Activity of Saponins Isolated from Vernonia gratiosa Hance

  • Pham Van Cong (Graduate University of Science and Technology, VAST) ;
  • Hoang Le Tuan Anh (Center for Research and Technology Transfer (CRTT), Vietnam Academy of Science and Technology (VAST)) ;
  • Le Ba Vinh (College of Pharmacy, Korea University) ;
  • Yoo Kyong Han (College of Pharmacy, Korea University) ;
  • Nguyen Quang Trung (Center for Research and Technology Transfer (CRTT), Vietnam Academy of Science and Technology (VAST)) ;
  • Bui Quang Minh (Center for Research and Technology Transfer (CRTT), Vietnam Academy of Science and Technology (VAST)) ;
  • Ngo Viet Duc (Center for Research and Technology Transfer (CRTT), Vietnam Academy of Science and Technology (VAST)) ;
  • Tran Minh Ngoc (Traditional Medicine Administration Ministry of Health) ;
  • Nguyen Thi Thu Hien (Hanoi University of Mining and Geology) ;
  • Hoang Duc Manh (National Institute of Medicinal Materials (NIMM)) ;
  • Le Thi Lien (Mientrung Institute for Scientific Research, VAST) ;
  • Ki Yong Lee (College of Pharmacy, Korea University)
  • Received : 2022.12.22
  • Accepted : 2023.02.14
  • Published : 2023.06.28
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Abstract

Species belonging to the Vernonia (Asteraceae), the largest genus in the tribe Vernonieae (consisting of about 1,000 species), are widely used in food and medicine. These plants are rich sources of bioactive sesquiterpene lactones and steroid saponins, likely including many as yet undiscovered chemical components. A phytochemical investigation resulted in the separation of three new stigmastane-type steroidal saponins (1 - 3), designated as vernogratiosides A-C, from whole plants of V. gratiosa. Their structures were elucidated based on infrared spectroscopy (IR), one-dimensional (1D) and two-dimensional nuclear magnetic resonance (2D NMR), high-resolution electrospray ionization mass spectrometry (HR-ESI-MS), and electronic circular dichroism analyses (ECD), as well as chemical reactivity. Molecular docking analysis of representative saponins with α-glucosidase inhibitory activity was performed. Additionally, the intended substances were tested for their ability to inhibit α-glucosidase activity in a laboratory setting. The results suggested that stigmastane-type steroidal saponins from V. gratiosa are promising candidate antidiabetic agents.

Keywords

Acknowledgement

This work was financially supported by Vietnam National Foundation for Science and Technology Development (NAFOSTED) under grant number 104.01-2020.11 and the National Research Foundation of Korea Grant funded by the Korean Government (NRF-2019R1A6A1A03031807 and NRF-2021R1A2C1093814).

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