Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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A Simple and Rapid Methicillin-Resistant Staphylococcus aureus (MRSA) Screening Test Using a Mannose-Binding Lectin (MBL)-Conjugated Gold Nanoparticle Probe

  • So Yeon Yi (Bionanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB) ) ;
  • Jinyoung Jeong (Environmental Disease Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Wang Sik Lee (Environmental Disease Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Jungsun Kwon (BioNano Health Guard Research Center) ;
  • Kyungah Yoon (Department of Clinical Pathology, Daejeon Health Institute of Technology) ;
  • Kyoungsook Park (Department of Biopharmacy, Daejeon Health Institute of Technology)
  • Received : 2023.01.02
  • Accepted : 2023.02.23
  • Published : 2023.05.28
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Abstract

Rapid diagnosis of methicillin-resistant Staphylococcus aureus (MRSA) is essential for guiding clinical treatment and preventing the spread of MRSA infections. Herein, we present a simple and rapid MRSA screening test based on the aggregation effect of mannose-binding lectin (MBL)-conjugated gold nanoparticles (AuNP), called the MRSA probe. Recombinant MBL protein is a member of the lectin family and part of the innate immune system. It can recognize wall teichoic acid (WTA) on the membrane of MRSA more specifically than that of methicillin-sensitive Staphylococcus aureus (MSSA) under optimized salt conditions. Thus, the MRSA probe can selectively bind to MRSA, and the aggregation of the probes on the surface of the target bacteria can be detected and analyzed by the naked eye within 5 min. To demonstrate the suitability of the method for real-world application, we tested 40 clinical S. aureus isolates (including 20 MRSA specimens) and recorded a sensitivity of 100%. In conclusion, the MRSA probe-based screening test with its excellent sensitivity has the potential for successful application in the microbiology laboratory.

Keywords

Acknowledgement

This study was supported by BioNano Health Guard Research Center funded by the Ministry of Science and ICT (MSIT) of Korea as a Global Frontier Project (Grant No. H-GUARD_2013 M3A6B2078950), the KRIBB Initiative Research Program (KGM5322321), and Daejeon Health Institute of Technology in 2022. The authors thank Prof. Yong. D. from the Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine (Korea), for providing clinical S. aureus isolates.

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