Journal of Microbiology and Biotechnology

  • 제33권5호
  • /
  • Pages.656-661
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  • 2023
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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Characterization of Low-Molecular-Weight Collagen from Korean Native Chicken Feet Hydrolyzed Using Alcalase

  • Heedong Woo (SCM Division, ASK COMPANY) ;
  • Gyeong A Jeong (Department of Food Science and Biotechnology, Wonkwang University) ;
  • Hyunwook Choi (Department of Functional Food and Biotechnology, Jeonju University) ;
  • Chang Joo Lee (Department of Food Science and Biotechnology, Wonkwang University)
  • 투고 : 2022.12.28
  • 심사 : 2023.01.12
  • 발행 : 2023.05.28
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초록

The aims of this study were to optimize the preparation of low-molecular-weight collagen using a proteolytic enzyme (alcalase) derived from the feet of Korean native chickens, and to characterize the process of collagen hydrolysis. Foreign bodies from chicken feet were removed using ultrasonication at 28 kHz with 1.36 kW for more than 25 min. The hydrolytic pattern and molecular weight distribution of enzyme-treated collagen from chicken feet were analyzed using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and high-performance liquid chromatography, respectively. Ideally, chicken feet should be treated at 100℃ for 8 h to obtain a high collagen content using hot water extraction. The collagen content of the chicken foot extract was 13.9 g/100 g, and the proportion of low-molecular-weight collagen increased with increasing proteolytic enzyme concentration and reaction time. When treated with 1% alcalase, the average molecular weight of collagen decreased rapidly to 4,929 Da within 5 h and thereafter decreased at a slower rate, reaching 4,916 Da after 7 h. Size exclusion chromatography revealed that low-molecular-weight collagen peptides of approximately 1,000-5,000 Da were obtained after hydrolysis with 1% alcalase for 1 h.

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과제정보

This study was supported by Wonkwang University in 2022.

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