Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Antimicrobial and Cytotoxic Activity of Endophytic Fungi from Lagopsis supina

  • Dekui Zhang (Department of Microbiology, College of Life Science, Key Laboratory for Agriculture Microbiology, Shandong Agricultural University) ;
  • Weijian Sun (College of Life Science, Shandong First Medical University & Shandong Academy of Medical Sciences) ;
  • Wenjie Xu (College of Life Science, Shandong First Medical University & Shandong Academy of Medical Sciences) ;
  • Changbo Ji (College of Life Science, Shandong First Medical University & Shandong Academy of Medical Sciences) ;
  • Yang Zhou (College of Life Science, Shandong First Medical University & Shandong Academy of Medical Sciences) ;
  • Jingyi Sun (College of Life Science, Shandong First Medical University & Shandong Academy of Medical Sciences) ;
  • Yutong Tian (College of Life Science, Shandong First Medical University & Shandong Academy of Medical Sciences) ;
  • Yanling Li (College of Life Science, Shandong First Medical University & Shandong Academy of Medical Sciences) ;
  • Fengchun Zhao (Department of Microbiology, College of Life Science, Key Laboratory for Agriculture Microbiology, Shandong Agricultural University) ;
  • Yuan Tian (College of Life Science, Shandong First Medical University & Shandong Academy of Medical Sciences)
  • Received : 2022.11.28
  • Accepted : 2022.12.24
  • Published : 2023.04.28
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Abstract

In this study, five endophytic fungi belonging to the Aspergillus and Alternaria genera were isolated from Lagopsis supina. The antimicrobial activity of all fungal fermented extracts against Staphylococcus and Fusarium graminearum was tested using the cup-plate method. Among them, Aspergillus ochraceus XZC-1 showed the best activity and was subsequently selected for large-scale fermentation and bioactivity-directed separation of the secondary metabolites. Four compounds, including 2-methoxy-6-methyl-1,4-benzoquinone (1), 3,5-dihydroxytoluene (2), oleic acid (3), and penicillic acid (4) were discovered. Here, compounds 1 and 4 displayed anti-fungal activity against F. graminearum, F. oxysporum, F. moniliforme, F. stratum, Botrytis cinerea, Magnaporthe oryzae, and Verticillium dahlia with diverse MIC values (128-512 ㎍/ml), which were close to that of the positive control antifungal, actidione (64-128 ㎍/ml). Additionally, compounds 1 and 4 also exhibited moderate antibacterial activity against S. aureus, Listeria monocytogenes, Escherichia coli, and Salmonella enterica, with low MIC values (8-64 ㎍/ml). Moreover, compounds 1 and 4 displayed selective cytotoxicity against cancer cell lines as compared with the normal fibroblast cells. Therefore, this study proposes that the endophytic fungi from L. supina can potentially produce bioactive molecules to be used as lead compounds in drugs or agricultural antibiotics.

Keywords

Acknowledgement

This work was supported by the Youth Science Foundation Program of Shandong First Medical University (202201-034), Shandong Medical and Health Science and Technology Development Project (202101060623), the National Natural Science Foundation of China (21602152) and the Student Research Training Program of Shandong First Medical University (2022104391556). We thank Bullet Edits Limited for the linguistic editing and proofreading of the manuscript.

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