Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Probiotic Characteristics and Safety Assessment of Lacticaseibacillus casei KGC1201 Isolated from Panax ginseng

  • Yun-Seok Lee (Laboratory of Products, Korea Ginseng Corporation) ;
  • Hye-Young Yu (Laboratory of Efficacy Research, Korea Ginseng Corporation) ;
  • Mijin Kwon (Laboratory of Efficacy Research, Korea Ginseng Corporation) ;
  • Seung-Ho Lee (Laboratory of Efficacy Research, Korea Ginseng Corporation) ;
  • Ji-In Park (Science Instrumentation Assessment and Application Team, Korea Basic Science Institute (KBSI)) ;
  • Jiho Seo (Laboratory of Analysis, Korea Ginseng Corporation) ;
  • Sang-Kyu Kim (Laboratory of Efficacy Research, Korea Ginseng Corporation)
  • Received : 2022.11.14
  • Accepted : 2023.01.25
  • Published : 2023.04.28
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Abstract

Panax ginseng is one of the most important herbal medicinal plants consumed as health functional food and can be fermented to achieve better efficacy. Lacticaseibacillus, one of the representative genera among lactic acid bacteria (LAB), has also been used as a probiotic material for health functional foods due to its beneficial effects on the human body. To achieve a synergistic effect by using these excellent dietary supplement ingredients together, a novel LAB strain was isolated from the root of 6-year-old ginseng. Through similarity analysis of 16S rRNAs and whole-genome sequences, the strain was confirmed as belonging to the genus Lacticaseibacillus and was named L. casei KGC1201. KGC1201 not only met all safety standards as food, but also showed excellent probiotic properties such as acid resistance, bile salt resistance, and intestinal adhesion. In particular, KGC1201 exhibited superior acid resistance through morphological observation identifying that the cell surface damage of KGC1201 was less than that of the L. casei type strain KCTC3109. Gene expression studies were conducted to elucidate the molecular mechanisms of KGC1201's acid resistance, and the expression of the glycosyltransferase gene was found to be significantly elevated under acidic conditions. Exopolysaccharides (EPSs) biosynthesized by glycosyltransferase were also increased in KGC1201 compared to KCTC3109, which may contribute to better protection of KGC1201 cells from strong acidity. Therefore, KGC1201, with its increased acid resistance through molecular mechanisms and excellent probiotic properties, can be used in health functional foods to provide greater benefit to overall human health and well-being.

Keywords

Acknowledgement

We thank Ms. J.-I. Park for helpful analyses and discussions with FE-SEM (Hitachi, Japan) at the Korea Basic Science Institute (KBSI).

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