International Journal of Stem Cells

  • 제16권1호
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  • Pages.27-35
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  • 2023
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  • 2005-3606(pISSN)
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  • 2005-5447(eISSN)
한국줄기세포학회 (Korean Society for Stem Cell Research)
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Inhibition of Class I Histone Deacetylase Enhances Self-Reprogramming of Spermatogonial Stem Cells into Pluripotent Stem Cells

  • Yukyeong Lee (Department of Stem Cell Biology, Konkuk University School of Medicine) ;
  • Seung-Won Lee (Department of Stem Cell Biology, Konkuk University School of Medicine) ;
  • Dahee Jeong (Department of Stem Cell Biology, Konkuk University School of Medicine) ;
  • Hye Jeong Lee (Department of Stem Cell Biology, Konkuk University School of Medicine) ;
  • Na Young Choi (Department of Stem Cell Biology, Konkuk University School of Medicine) ;
  • Jin Seok Bang (Department of Stem Cell Biology, Konkuk University School of Medicine) ;
  • Seokbeom Ham (Department of Stem Cell Biology, Konkuk University School of Medicine) ;
  • Kinarm, Ko (Department of Stem Cell Biology, Konkuk University School of Medicine)
  • 투고 : 2022.07.05
  • 심사 : 2022.09.21
  • 발행 : 2023.02.28
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초록

Background and Objectives: Spermatogonial stem cells (SSCs) are the most primitive cells in spermatogenesis and are the only adult stem cells capable of passing on the genome of a given species to the next generation. SSCs are the only adult stem cells known to exhibit high Oct4 expression and can be induced to self-reprogram into pluripotent cells depending on culture conditions. Epigenetic modulation is well known to be involved in the induction of pluripotency of somatic cells. However, epigenetic modulation in self-reprogramming of SSCs into pluripotent cells has not been studied. Methods and Results: In this study, we examined the involvement of epigenetic modulation by assessing whether selfreprogramming of SSCs is enhanced by treatment with epigenetic modulators. We found that second-generation selective class I HDAC inhibitors increased SSC reprogramming efficiency, whereas non-selective HDAC inhibitors had no effect. Conclusions: We showed that pluripotent stem cells derived from adult SSCs by treatment with small molecules with epigenetic modulator functions exhibit pluripotency in vitro and in vivo. Our results suggest that the mechanism of SSC reprogramming by epigenetic modulator can be used for important applications in epigenetic reprogramming research.

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과제정보

This study was supported by Konkuk University in 2021.

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