Korean Chemical Engineering Research

The Korean Institute of Chemical Engineers (한국화학공학회)
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Urea Conversion via Enzymes Immobilized on Magnetic Microparticles

자성 미세입자에 고정된 효소를 통한 요소 전환

  • Yeseul Park (Department of Chemical and Biomolecular Engineering, College of Energy and Biotechnology, Seoul National University of Science and Technology) ;
  • Jieun Kwon (Department of Chemical and Biomolecular Engineering, College of Energy and Biotechnology, Seoul National University of Science and Technology) ;
  • Seungjun Jung (Department of Chemical and Biomolecular Engineering, College of Energy and Biotechnology, Seoul National University of Science and Technology) ;
  • Hyukjin Cho (Department of Chemical and Biomolecular Engineering, College of Energy and Biotechnology, Seoul National University of Science and Technology) ;
  • Gounhanul Shin (Department of Chemical and Biomolecular Engineering, College of Energy and Biotechnology, Seoul National University of Science and Technology) ;
  • Gangik Cho (Department of Chemical and Biomolecular Engineering, College of Energy and Biotechnology, Seoul National University of Science and Technology) ;
  • Jin-Won Park (Department of Chemical and Biomolecular Engineering, College of Energy and Biotechnology, Seoul National University of Science and Technology) ;
  • Kyung-Hyuk Lee (K-water Research Institute Water & Wastewater Research Center)
  • 박예슬 (서울과학기술대학교 화공생명공학과) ;
  • 권지은 (서울과학기술대학교 화공생명공학과) ;
  • 정승준 (서울과학기술대학교 화공생명공학과) ;
  • 조혁진 (서울과학기술대학교 화공생명공학과) ;
  • 신고운하늘 (서울과학기술대학교 화공생명공학과) ;
  • 조강익 (서울과학기술대학교 화공생명공학과) ;
  • 박진원 (서울과학기술대학교 화공생명공학과) ;
  • 이경혁 (K-water연구원 K-water)
  • Received : 2023.05.18
  • Accepted : 2023.06.05
  • Published : 2023.08.01
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Abstract

The urease is covalently immobilized on the surface of the magnetic particles to catalyze the conversion of urea to bicarbonate anion. The conversion was confirmed qualitatively using high-pressure liquid chromatography and UV/Vis spectrometry, and analyzed quantitatively with cyclic voltammetry. The amount of conversion with respect to time was measured and analyzed by the reaction rate equation to calculate a reaction rate constant of 0.0474 min-1. In the 1 to 3 cycles, a conversion percentage of over 90% was found, and it was possible to reuse the urease 8 times up to the percentage of 50%. It was also observed that the stability evaluated for storage for 30 days was maintained. As a result of this study, it can be seen that the urease covalently immobilized on the scaffold can be used for urea removal for the purpose of producing ultrapure water.

요소분해효소가 자성입자표면에 공유결합으로 고정되어 요소의 중탄산음이온 전환을 촉진하였으며, 이 전환을 정성적으로 고압액체크로마토그래피와 UV/Vis분광법으로 확인하고 순환전압전류법을 사용하여 정량적으로 분석하였다. 시간에 따른 전환량을 측정하고 이를 반응속도식으로 분석하여 0.0474 min-1의 반응속도 상수를 산출하였다. 처음 1~3회에서는 90% 이상의 전환율을 확인하였으며, 50% 전환율까지 8회 재활용이 가능하였다. 30일 동안의 저장에 대하여 평가된 안정성은 유지되는 것으로 관찰되었다. 본 연구의 결과, 지지체에 공유결합으로 고정된 요소분해효소는 초순수 제조 목적으로 요소제거에 활용될 수 있음을 알 수 있다.

Keywords

Acknowledgement

본 연구는 한국수자원공사(K-water)의 개방형 혁신 R&D (OTSK_2022_003) 사업의 일환으로 수행되었습니다.

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