Journal of Ginseng Research

The Korean Society of Ginseng (고려인삼학회)
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Ginsenosides Rg1 regulate lipid metabolism and temperature adaptation in Caenorhabditis elegans

  • Hao Shi (School of Traditional Chinese Medicine, Southern Medical University) ;
  • Jiamin Zhao (School of Traditional Chinese Medicine, Southern Medical University) ;
  • Yiwen Li (School of Traditional Chinese Medicine, Southern Medical University) ;
  • Junjie Li (School of Traditional Chinese Medicine, Southern Medical University) ;
  • Yunjia Li (School of Traditional Chinese Medicine, Southern Medical University) ;
  • Jia Zhang (School of Traditional Chinese Medicine, Southern Medical University) ;
  • Zhantu Qiu (School of Traditional Chinese Medicine, Southern Medical University) ;
  • Chaofeng Wu (School of Traditional Chinese Medicine, Southern Medical University) ;
  • Mengchen Qin (School of Traditional Chinese Medicine, Southern Medical University) ;
  • Chang Liu (School of Traditional Chinese Medicine, Southern Medical University) ;
  • Zhiyun Zeng (School of Traditional Chinese Medicine, Southern Medical University) ;
  • Chao Zhang (Department of Biochemistry, School of Basic Medical Sciences, Southern Medical University) ;
  • Lei Gao (School of Traditional Chinese Medicine, Southern Medical University)
  • Received : 2022.08.03
  • Accepted : 2022.11.03
  • Published : 2023.07.01
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Abstract

Background: Obesity is a risk factor for aging and many diseases, and the disorder of lipid metabolism makes it prominent. This study aims to investigate the effect of ginsenoside Rg1 on aging, lipid metabolism and stress resistance Methods: Rg1 was administered to Caenorhabditis elegans (C. elegans) cultured in NGM or GNGM. The lifespan, locomotory activity, lipid accumulation, cold and heat stress resistance and related mRNA expression of the worms were examined. Gene knockout mutants were used to clarify the effect on lipid metabolism of Rg1. GFP-binding mutants were used to observe the changes in protein expression Results: We reported that Rg1 reduced lipid accumulation and improved stress resistance in C. elegans. Rg1 significantly reduced the expression of fatty acid synthesis-related genes and lipid metabolism-related genes in C. elegans. However, Rg1 did not affect the fat storage in fat-5/fat-6 double mutant or nhr-49 mutant. Combined with network pharmacology, we clarified the possible pathways and targets of Rg1 in lipid metabolism. In addition, Rg1-treated C. elegans showed a higher expression of anti-oxidative genes and heat shock proteins, which might contribute to stress resistance Conclusion: Rg1 reduced fat accumulation by regulating lipid metabolism via nhr-49 and enhanced stress resistance by its antioxidant effect in C. elegans.

Keywords

Acknowledgement

The above study was financially supported by the National Natural Science Foundation of China (82074131, 81774170), the Natural Science Foundation of Guangdong Province (2018B030306012), Guangzhou Basic and Applied Basic Research Project (202201011800), the Outstanding Youth Development Scheme project of Southern Medical University (G621299870), Young Elite Scientists Sponsorship Program by CACM (2021-QNRC2-B28).

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