Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Complete Genome Sequence of a Staphylococcus aureus Sequence Type 188 Strain SA73-2 Isolated from Lettuce

  • Kwang-Kyo Oh (Microbial Safety Division, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Seung-Mi Seo (Microbial Safety Division, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Hyunsuk Jin (Microbial Safety Division, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Bo-Eun Kim (Microbial Safety Division, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Husna (Microbial Safety Division, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Dong Suk Park (Microbial Safety Division, National Institute of Agricultural Sciences, Rural Development Administration)
  • Received : 2023.10.31
  • Accepted : 2023.12.20
  • Published : 2023.12.28
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Abstract

Staphylococcus aureus is a ubiquitous pathogen associated with both human and animal diseases. In this study, we present the complete genome sequence of SA73-2, which was isolated from lettuce at an agricultural farm in South Korea. The assembled genome consists of a 2,750,304-bp circular chromosome and a 24,654-bp plasmid. This strain belongs to sequence type 188 (ST188), with spa type 189, and carries the β-lactamase gene (blaZ) on the plasmid.

Keywords

Acknowledgement

This work was supported by the Research Program for Agriculture Science and Technology Development (Project No. PJ016298) of the National Institute of Agricultural Sciences, Rural Development Administration, Republic of Korea.

References

  1. Li W, Liu J-H, Zhang X-F, Wang J, Ma Z-B, Chen L, et al. 2018. Emergence of methicillin-resistant Staphylococcus aureus ST398 in pigs in China. Int. J. Antimicrob. Agents 51: 275-276. https://doi.org/10.1016/j.ijantimicag.2017.10.013
  2. Abdalrahman LS, Stanley A, Wells H, Fakhr MK. 2015. Isolation, virulence, and antimicrobial resistance of methicillin-resistant Staphylococcus aureus (MRSA) and methicillin sensitive Staphylococcus aureus (MSSA) strains from Oklahoma retail poultry meats. Int. J. Environ. Res. Public Health 12: 6148-6161. https://doi.org/10.3390/ijerph120606148
  3. Chin C-S, Alexander DH, Marks P, Klammer AA, Drake J, Heiner C, et al. 2013. Nonhybrid, finished microbial genome assemblies from long-read SMRT sequencing data. Nat. Methods 10: 563-569. https://doi.org/10.1038/nmeth.2474
  4. Walker BJ, Abeel T, Shea T, Priest M, Abouelliel A, Sakthikumar S, et al. 2014. Pilon: an integrated tool for comprehensive microbial variant detection and genome assembly improvement. PLoS One 9: e112963.
  5. Seemann T. 2014. Prokka: rapid prokaryotic genome annotation. Bioinformatics 30: 2068-2069. https://doi.org/10.1093/bioinformatics/btu153
  6. Jones P, Binns D, Chang H-Y, Fraser M, Li W, McAnulla C, et al. 2014. InterProScan 5: genome-scale protein function classification. Bioinformatics 30: 1236-1240. https://doi.org/10.1093/bioinformatics/btu031
  7. Huerta-Cepas J, Szklarczyk D, Forslund K, Cook H, Heller D, Walter MC, et al. 2016. eggNOG 4.5: a hierarchical orthology framework with improved functional annotations for eukaryotic, prokaryotic and viral sequences. Nucleic Acids Res. 44: D286-D293. https://doi.org/10.1093/nar/gkv1248
  8. (CLSI) CaLSI. 2022. Performance Standards for Antimicrobial Susceptibility Testing M100 32th edition.
  9. Lee I, Kim YO, Park S-C, Chun J. 2016. OrthoANI: an improved algorithm and software for calculating average nucleotide identity. Int. J. Syst. Evol. Microbiol. 66: 1100-1103. https://doi.org/10.1099/ijsem.0.000760