DOI QR코드

DOI QR Code

Draft Genome Sequence of Latilactobacillus sakei subsp. sakei FBL10, a Putative Probiotic Strain Isolated from Saeujeot (salted fermented shrimp)

  • So-Yun Lee (Institute of Life Sciences and Resources and Department of Food Science and Biotechnology, Kyung Hee University) ;
  • Dabin Kim (Institute of Life Sciences and Resources and Department of Food Science and Biotechnology, Kyung Hee University) ;
  • Seung-Min Yang (Institute of Life Sciences and Resources and Department of Food Science and Biotechnology, Kyung Hee University) ;
  • Eiseul Kim (Institute of Life Sciences and Resources and Department of Food Science and Biotechnology, Kyung Hee University) ;
  • Hae-Yeong Kim (Institute of Life Sciences and Resources and Department of Food Science and Biotechnology, Kyung Hee University)
  • 투고 : 2023.09.19
  • 심사 : 2023.10.20
  • 발행 : 2023.12.28

초록

Here, we report the draft genome sequence of Latilactobacillus sakei subsp. sakei FBL10 isolated from Saeujeot (salted fermented shrimp). The draft genome consists of 2,285,672 bp with a G+C content of 41.1% and contains 2,282 coding genes. Genome analysis revealed that clusters associated with bacteriocin production were identified, in addition to several probiotic properties, such as stress resistance factors and aggregation. On the other hand, antibiotic resistance genes and virulence factors were not present. Pangenome analysis for 32 genomes showed 213 unique genes for FBL10 strain. These results demonstrate the beneficial properties of strain FBL10 as a putative probiotic.

키워드

과제정보

This research is funded by Graduate School Innovation office, Kyung Hee University (2023).

참고문헌

  1. Zagorec M, Champomier-Verges MC. 2017. Lactobacillus sakei: A starter for sausage fermentation, a protective culture for meat products. Microorganisms 5: 56. 
  2. McLeod A, Zagorec M, Champomier-Verges MC, Naterstad K, Axelsson L. 2010. Primary metabolism in Lactobacillus sakei food isolates by proteomic analysis. BMC Microbiol. 10: 120. 
  3. Kim H, Rather IA, Kim H, Kim S, Kim T, Jang J, et al. 2015. A double-blind, placebo controlled-trial of a probiotic strain Lactobacillus sakei probio-65 for the prevention of canine atopic dermatitis. J. Microbiol. Biotechnol. 25: 1966-1969.  https://doi.org/10.4014/jmb.1506.06065
  4. Chaillou S, Champomier-Verges MC, Cornet M, Crutz-Le Coq AM, Dudez AM, Martin V, et al. 2005. The complete genome sequence of the meat-borne lactic acid bacterium Lactobacillus sakei 23K. Nat. Biotechnol. 23: 1527-1533.  https://doi.org/10.1038/nbt1160
  5. Montanari C, Barbieri F, Magnani M, Grazia L, Gardini F, Tabanelli G. 2018. Phenotypic diversity of Lactobacillus sakei strains. Front. Microbiol. 9: 2003. 
  6. Kim E, Yang SM, Kim D, Kim HY. 2022. Complete genome sequencing and comparative genomics of three potential probiotic strains, Lacticaseibacillus casei FBL6, Lacticaseibacillus chiayiensis FBL7, and Lacticaseibacillus zeae FBL8. Front. Microbiol. 12: 794315. 
  7. Qureshi N, Gu Q, Li P. 2020. Whole genome sequence analysis and in vitro probiotic characteristics of a Lactobacillus strain Lactobacillus paracasei ZFM54. J. Appl. Microbiol. 129: 422-433.  https://doi.org/10.1111/jam.14627
  8. Abriouel H, Perez Montoro B, Casimiro-Soriguer CS, Perez Pulido AJ, Knapp CW, Caballero Gomez N, et al. 2017. Insight into potential probiotic markers predicted in Lactobacillus pentosus MP-10 genome sequence. Front. Microbiol. 8: 891. 
  9. Sturme MHJ, Nakayama J, Molenaar D, Murakami Y, Kunugi R, Fujii T, et al. 2005. An agr-like two-component regulatory system in Lactobacillus plantarum is involved in production of a novel cyclic peptide and regulation of adherence. J. Bacteriol. 187: 5224-5235.  https://doi.org/10.1128/JB.187.15.5224-5235.2005
  10. Li Y, Rebuffat S. 2020. The manifold roles of microbial ribosomal peptide-based natural products in physiology and ecology. J. Biol. Chem. 295: 34-54. https://doi.org/10.1074/jbc.REV119.006545