한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제51권4호
  • /
  • Pages.484-499
  • /
  • 2023
  • /
  • 1598-642X(pISSN)
  • /
  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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Isolation and Characterization of Plant Growth-Promoting Bacteria for the Phytoremediation of Diesel- and Heavy Metal-Contaminated Soil

  • Yun-Yeong Lee (Department of Environmental Science and Engineering, Ewha Womans University) ;
  • Kyung-Suk Cho (Department of Environmental Science and Engineering, Ewha Womans University)
  • 투고 : 2023.08.10
  • 심사 : 2023.10.11
  • 발행 : 2023.12.28
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초록

Plant growth-promoting (PGP) bacteria can be used as bioresources to enhance phytoremediation through their PGP traits and pollutant removal capacity. In this study, 49 rhizobacteria were primarily isolated from the rhizosphere of tall fescue grown in diesel- and heavy metal-contaminated soil. Their biosurfactant production, phosphate (P) solubilization, and siderophore production were qualitatively and quantitatively evaluated to identify superior PGP bacteria. The optimal conditions for the growth of PGP bacteria and the stability of their PGP traits were a temperature of 35℃, a pH of 7, and 2 days of cultivation time. Four superior PGP bacteria (Pseudomonas sp. NL3, Bacillus sp. NL6, Bacillus sp. LBY14, and Priestia sp. TSY6) were finally selected. Pseudomonas sp. NL3 exhibited superior biosurfactant production and P solubilization. Bacillus sp. NL6 showed the highest P solubilization and superior production of biosurfactants and siderophores. Bacillus sp. LBY14 offered the best siderophore production and impressive P solubilization. Priestia sp. TSY6 had superior capacity for all three PGP traits. Through their secretion of beneficial PGP metabolites, the four bacteria isolated in this study have the potential for use in the phytoremediation of contaminated soil.

키워드

과제정보

This study was supported by a National Research Foundation of Korea (NRF) grant funded by the Korean government, the Ministry of Science and ICT (MICT) (2019R1A2C2006701 & 2022R1A2C2006615).

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