DOI QR코드

DOI QR Code

Whole Genome Sequence of Streptomyces sp. from Novel Marine Actinomycetes

  • Hyeon Kyeong Lee (Department of Biological Sciences and Bioengineering, Inha University) ;
  • Heung-Soon Park (Department of Biological Sciences and Bioengineering, Inha University) ;
  • Eung-Soo Kim (Department of Biological Sciences and Bioengineering, Inha University) ;
  • Si-Sun Choi (Department of Biological Sciences and Bioengineering, Inha University)
  • 투고 : 2023.06.05
  • 심사 : 2023.07.07
  • 발행 : 2023.09.28

초록

This paper presents the complete genome sequence of a novel marine actinomycete, Streptomyces sp. MMBL 11-1. The genome of Streptomyces sp. MMBL 11-1 was obtained through next-generation sequencing using the PacBio Sequel system and Illumina platform provided by Macrogen, Korea. The assembled genome consists of five contigs, with a total length of 8,496,900 bp and a G+C content of 71.6%. The genome harbors multiple biosynthetic gene clusters (BGCs) associated with producing microbial natural products (MNPs). The comprehensive genomic information of this type of strain will serve as a valuable resource for identifying other marine actinomycetes strains.

키워드

과제정보

The authors appreciate the marine-sponge sample provided by the KOIST. This work was funded by the National Research Foundation of Korea (Project No. NRF-2021R1A2C2012203).

참고문헌

  1. Dharmaraj S. 2010. Marine Streptomyces as a novel source of bioactive substances. Springer 26: 2123-2125.  https://doi.org/10.1007/s11274-010-0415-6
  2. Li X, Qin L. 2005. Metagenomics-based drug discovery and marine microbial diversity. Trends Biotechnol. 23: 539-543.  https://doi.org/10.1016/j.tibtech.2005.08.006
  3. Chin CS, Alexander DH, Marks P, Klammer AA, Drake J, Heiner C, et al. 2013. Nonhybrid, finished microbial genome assemblies from long-read SMRT sequencing data. Nat. Methods 10: 563-569.  https://doi.org/10.1038/nmeth.2474
  4. Walker BJ, Abeel T, Shea T, Priest M, Abouelliel A, Sakthikumar S, et al. 2014. Pilon: An integrated tool for comprehensive microbial variant detection and genome assembly improvement. PLoS One 9: e112963. 
  5. Seemann T. 2014. Prokka: rapid prokaryotic genome annotation. Bioinformatics 30: 2068-2069.  https://doi.org/10.1093/bioinformatics/btu153
  6. Jones P, Binns D, Chang HY, Fraser M, Li W, McAnulla C, et al. 2014. InterProScan 5: genome-scale protein function classification. Bioinformatics 30: 1236-1240.  https://doi.org/10.1093/bioinformatics/btu031
  7. Camacho C, Coulouris G, Avagyan V, Ma N, Papadopoulos J, Bealer K, et al. 2009. BLAST+: architecture and applications. BMC Bioinformatics. 10: 421. 
  8. Huerta-Cepas J, Szklarczyk D, Forslund K, Cook H, Heller D, Walter MC, et al. 2016. eggNOG 4.5: a hierarchical orthology framework with improved functional annotations for eukaryotic, prokaryotic and viral sequences. Nucleic Acids Res. 44: D286-293.  https://doi.org/10.1093/nar/gkv1248
  9. Krzywinski M, Schein J, Birol I, Connors J, Gascoyne R, Horsman D, et al. 2009. Circos: An information aesthetic for comparative genomics. Genome Res. 19: 1639-1645.  https://doi.org/10.1101/gr.092759.109
  10. Saitou N, Nei M. 1987. The neighbor-joining method: A new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4: 406-425.