한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제51권3호
  • /
  • Pages.317-324
  • /
  • 2023
  • /
  • 1598-642X(pISSN)
  • /
  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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Deciphering Key Genes of Proliferative and Secretory Phase Using Integrated Transcriptomics and Network Analysis

  • Payal Gupta (Amity Institute of Biotechnology, Amity University Uttar Pradesh) ;
  • Shriya Dube (Amity Institute of Biotechnology, Amity University Uttar Pradesh) ;
  • Payal Priyadarshini (Amity Institute of Biotechnology, Amity University Uttar Pradesh) ;
  • Shanvi Singh (Amity Institute of Biotechnology, Amity University Uttar Pradesh) ;
  • Anasuya Pravallika R (Amity Institute of Biotechnology, Amity University Uttar Pradesh) ;
  • Vijay Lakshmi Srivastava (Amity Institute of Biotechnology, Amity University Uttar Pradesh) ;
  • Abhishek Sengupta (Amity Institute of Biotechnology, Amity University Uttar Pradesh) ;
  • Priyanka Narad (Amity Institute of Biotechnology, Amity University Uttar Pradesh)
  • 투고 : 2023.04.26
  • 심사 : 2023.06.28
  • 발행 : 2023.09.28
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초록

Endometrium receptivity is a complex mechanism of intricate pathways that lead to the shift from the proliferative to the secretory phase. Our goal was to identify high-ranking differentially expressed genes and study the pathways associated with the phenomenon. Raw data were retrieved from six GEO datasets and 705 DEGs were identified through robust ranking aggregation after the integration of five datasets. 20 key genes were identified that were further re-validated in an additional dataset. Supporting evidence through the experimental references confirms them as major biomarkers of the shift from the proliferative to the secretory phase.

키워드

과제정보

We would like to acknowledge Dr. Ashok K. Chauhan, Founder, and President, Amity University, Uttar Pradesh for providing us the opportunity to conduct research. We would also like to thank Centre for Computational Biology and Bioinformatics, Amity Institute of Biotechnology, Amity University for providing us with necessary resources.

참고문헌

  1. Petracco RG, Kong A, Grechukhina O, Krikun G, Taylor HS. 2012. Global gene expression profiling of proliferative phase endometrium reveals distinct functional subdivisions. Reprod. Sci. 19: 1138-1145.  https://doi.org/10.1177/1933719112443877
  2. Yanaihara A, Otsuka Y, Iwasaki S, Aida T, Tachikawa T, Irie T, et al. 2005. Differences in gene expression in the proliferative human endometrium. Fertil. Steril. 83: 1206-1215.  https://doi.org/10.1016/j.fertnstert.2004.11.032
  3. Kelleher AM, Behura SK, Burns GW, Young SL, Demayo FJ, Spencer TE. 2019. Integrative analysis of the forkhead box A2 (FOXA2) cistrome for the human endometrium. FASEB J. 33: 8543-8554.  https://doi.org/10.1096/fj.201900013R
  4. Geo accession viewer. National Center for Biotechnology Information. https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE29981 [Accessed January 24, 2023]. 
  5. Altmae S, Reimand J, Hovatta O, Zhang P, Kere J, Laisk T, et al. 2012. Research resource: Interactome of Human embryo implantation: Identification of gene expression pathways, regulation, and integrated regulatory networks. Mol. Endocrinol. 26: 203-217.  https://doi.org/10.1210/me.2011-1196
  6. Chi R-pin A, Wang T, Adams N, Wu S-pin, Young SL, Spencer TE, et al. 2019. Human endometrial transcriptome and progesterone receptor cistrome reveal important pathways and epithelial regulators. J. Clin. Endocrinol. Metab. 105: e1419-e1439.  https://doi.org/10.1210/clinem/dgz117
  7. Giacomini E, Scotti GM, Vanni VS, Lazarevic D, Makieva S, Privitera L, et al. 2021. Global transcriptomic changes occur in uterine fluid-derived extracellular vesicles during the endometrial window for embryo implantation. Hum. Reprod. 36: 2249-2274.  https://doi.org/10.1093/humrep/deab123
  8. Sigurgeirsson B, Amark H, Jemt A, Ujvari D, Westgren M, Lundeberg J, et al. 2017. Comprehensive RNA sequencing of healthy human endometrium at two time points of the menstrual cycle. Biol. Reprod. 96: 24-33. 
  9. Salamonsen Lois A, Jennifer C Hutchison, Caroline E. Gargett. 2021. Cyclical endometrial repair and regeneration. Development 148: Dev199577.