한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제51권3호
  • /
  • Pages.257-267
  • /
  • 2023
  • /
  • 1598-642X(pISSN)
  • /
  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지
DOI QR코드

DOI QR Code

Valorization of Pineapple Peel Waste for Sustainable Polyhydroxyalkanoates Production

  • Kannika Bunkaew (Microbial Technology for Agriculture, Food and Environment Research Center, Faculty of Science, Thaksin University) ;
  • Kittiya Khongkool (Microbial Technology for Agriculture, Food and Environment Research Center, Faculty of Science, Thaksin University) ;
  • Monthon Lertworapreecha (Microbial Technology for Agriculture, Food and Environment Research Center, Faculty of Science, Thaksin University) ;
  • Kamontam Umsakul (Division of Biological Sciences, Faculty of Science, Prince of Songkla University) ;
  • Kumar Sudesh (Ecobiomaterial Research Laboratory, School of Biological Sciences, Universiti Sains Malaysia) ;
  • Wankuson Chanasit (Microbial Technology for Agriculture, Food and Environment Research Center, Faculty of Science, Thaksin University)
  • 투고 : 2023.05.30
  • 심사 : 2023.07.31
  • 발행 : 2023.09.28
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

The potential polyhydroxyalkanoates (PHA)-producing bacteria, Bacillus megaterium PP-10, was successfully isolated and studied its feasibility for utilization of pineapple peel waste (PPW) as a cheap carbon substrate. The PPW was pretreated with 1% (v/v) H2SO4 under steam sterilization and about 26.4 g/l of total reducing sugar (TRS) in pineapple peel hydrolysate (PPH) was generated and main fermentable sugars were glucose and fructose. A maximum cell growth and PHA concentration of 3.63 ± 0.07 g/l and 1.98 ± 0.09 g/l (about 54.58 ± 2.39%DCW) were received in only 12 h when grown in PPH. Interestingly, PHA productivity and biomass yield (Yx/s) in PPH was about 4 times and 1.5 times higher than in glucose. To achieve the highest DCW and PHA production, the optimal culture conditions e.g. carbon to nitrogen ratios of 40 mole/mole, incubation temperature at 35℃ and shaking speed of 200 rpm were performed and a maximum DCW up to 4.24 ± 0.04 g/l and PHA concentration of 2.68 ± 0.02 g/l (61% DCW) were obtained. The produced PHA was further examined its monomer composition and found to contain only 3-hydroxybutyrate (3HB). This finding corresponded with the presence of class IV PHA synthase gene. Finally, certain thermal properties of the produced PHA i.e. the melting temperature (Tm) and the glass transition temperature (Tg) were about 176℃ and -4℃, respectively whereas the Mw was about 1.07 KDa ; therefore, the newly isolated B. megaterium PP-10 is a promising bacterial candidate for the efficient conversion of low-cost PPH to PHA.

키워드

과제정보

This work was financially supported by National Higher Education, Science, Research and Innovation Policy Council, Thaksin University (Research project grant no. TSU-65A105000021) Fiscal Year 2022.

참고문헌

  1. Penkhrue W, Jendrossek D, Khanongnuch C, Pathom-Aree W, Aizawa T, Behrens RL, et al. 2020. Response surface method for polyhydroxybutyrate (PHB) bioplastic accumulation in Bacillus drentensis BP17 using pineapple peel. PLoS One 15: e0230443. 
  2. Sukruansuwan V, Napathorn SC. 2018. Use of agro-industrial residue from the canned pineapple industry for polyhydroxybutyrate production by Cupriavidus necator strain A-04. Biotechnol. Biofuels 11: 202. 
  3. Loo CY, Sudesh K. 2007. Polyhydroxyalkanoates: bio-based microbial plastics and their properties. Malaysian Polym. J. 2: 31-57. 
  4. Tan G-YA, Chen C-L, Li L, Ge L, Wang L, Razaad IMN, et al. 2014. A review: Start a research on biopolymer polyhydroxyalkanoate (PHA). Polymers 6: 706-754.  https://doi.org/10.3390/polym6030706
  5. Andhalkar VV, Ahorsu R, Dominguez de Maria P, Winterburn J, Medina F, Constanti M. 2022. Facts and challenges : Valorization of Lignocellulose by producing polyhydroxyalkanoates under circular bioeconomy premises. ACS Sustain. Chem. Eng. 10: 16459-16475.  https://doi.org/10.1021/acssuschemeng.2c04925
  6. Tsuge T, Hyakutake M, Mizuno K. 2015. Class IV polyhydroxyalkanoate (PHA) synthases and PHA-producing Bacillus. Appl. Microbiol. Biotechnol. 99: 6231-6240.  https://doi.org/10.1007/s00253-015-6777-9
  7. Rehm BH. 2010. Bacterial polymers: biosynthesis, modifications and applications. Nat. Rev. Microbiol. 8: 578-592.  https://doi.org/10.1038/nrmicro2354
  8. Biedendieck R, Knuuti T, Moore SJ, Jahn D. 2021. The "beauty in the beast"-the multiple uses of Priestia megaterium in biotechnology. Appl. Microbiol. Biotechnol. 105: 5719-5737.  https://doi.org/10.1007/s00253-021-11424-6
  9. Rodriguez-Contreras A, Koller M, Miranda-de Sousa Dias M, Calafell-Monfort M, Braunegg G, Marques-Calvo MS. 2013. High production of poly (3-hydroxybutyrate) from a wild Bacillus megaterium Bolivian strain. J. Appl. Microbiol. 114: 1378-1387.  https://doi.org/10.1111/jam.12151
  10. Gouda MK, Swellam AE, Omar SH. 2001. Production of PHB by a Bacillus megaterium strain using sugarcane molasses and corn steep liquor as sole carbon and nitrogen sources. Microbiol. Res. 156: 201-207.  https://doi.org/10.1078/0944-5013-00104
  11. Wang K, Zhang R. 2021. Production of polyhydroxyalkanoates (PHA) by Haloferax mediterranei from food waste derived nutrients for biodegradable plastic applications. J. Microbiol. Biotechnol. 31: 338-347.  https://doi.org/10.4014/jmb.2008.08057
  12. Suwannasing W, Imai T, Kaewkannetra P. 2015. Potential utilization of pineapple waste streams for polyhydroxyalkanoates (PHAs) production via batch fermentation. J. Water Environ. Technol. 13: 335-347.  https://doi.org/10.2965/jwet.2015.335
  13. Obruca S, Benesova P, Marsalek L, Marova I. 2015. Use of lignocellulosic materials for PHA production. Chem. Biochem. Eng. Q. 29: 135-144.  https://doi.org/10.15255/CABEQ.2014.2253
  14. Li J, Yang Z, Zhang K, Liu M, Liu D, Yan X, Si M, Shi Y. 2021. Valorizing waste liquor from dilute acid pretreatment of lignocellulosic biomass by Bacillus megaterium B-10. Ind. Crops Prod. 161: 113160. 
  15. Kulpreecha S, Boonruangthavorn A, Meksiriporn B, Thongchul N. 2009. Inexpensive fed-batch cultivation for high poly (3-hydroxybutyrate) production by a new isolate of Bacillus megaterium. J. Biosci. Bioeng. 107: 240-245.  https://doi.org/10.1016/j.jbiosc.2008.10.006
  16. Nasir K, Batool R, Jamil N. 2022. Scale-up studies for polyhydroxyalkanoate and halocin production by Halomonas sp. as potential biomedical materials. J. Biom. Biomater. Biomed. Eng. 56: 49-60.  https://doi.org/10.4028/p-yqf2wv
  17. Tian J, Sinskey AJ, Stubbe J. 2005. Kinetic studies of polyhydroxybutyrate granule formation in Wautersia eutropha H16 by transmission electron microscopy. J. Bacteriol. 187: 3814-3824.  https://doi.org/10.1128/JB.187.11.3814-3824.2005
  18. Kannan L, Wheeler WC. 2012. Maximum parsimony on phylogenetic networks. Algorithms Mol. Biol. 7: 9. 
  19. Halket G, Dinsdale A, Logan NA. 2010. Evaluation of the VITEK2 BCL card for identification of Bacillus species and other aerobic endosporeformers. Lett. Appl. Microbiol. 50: 120-126.  https://doi.org/10.1111/j.1472-765X.2009.02765.x
  20. Berekaa MM. 2012. Genotypic detection of polyhydroxyalkanoate-producing bacilli and characterization of phaC synthase of Bacillus sp. SW1-2. Life Sci. J. 4: 9. 
  21. Chanasit W, Hodgson B, Sudesh K, Umsakul K. 2016. Efficient production of polyhydroxyalkanoates (PHAs) from Pseudomonas mendocina PSU using a biodiesel liquid waste (BLW) as the sole carbon source. Biosci. Biotechnol. Biochem. 80: 1440-1450.  https://doi.org/10.1080/09168451.2016.1158628
  22. de Souza Hassemer G, Colet R, de Melo RN, Fischer B, Lin Y-H, Junges A, et al. 2021. Production of poly(3-hydroxybutyrate) (P (3HB)) from different agroindustry byproducts by Bacillus megaterium. Biointerface Res. Appl. Chem. 11: 14278-14289.  https://doi.org/10.33263/BRIAC116.1427814289
  23. Hamdy SM, Danial AW, Gad El-Rab SM, Shoreit AA, Hesham AE-L. 2022. Production and optimization of bioplastic (Polyhydroxybutyrate) from Bacillus cereus strain SH-02 using response surface methodology. BMC Microbiol. 22: 183. 
  24. Wong Y-M, Brigham CJ, Rha C, Sinskey AJ, Sudesh K. 2012. Biosynthesis and characterization of polyhydroxyalkanoate containing high 3-hydroxyhexanoate monomer fraction from crude palm kernel oil by recombinant Cupriavidus necator. Bioresour. Technol. 121: 320-327.  https://doi.org/10.1016/j.biortech.2012.07.015
  25. de Oliveira Schmidt VK, Santos EFd, de Oliveira D, Ayub MAZ, Cesca K, Cortivo PRD, et al. 2022. Production of polyhydroxyalkanoates by Bacillus megaterium: Prospecting on rice hull and residual glycerol potential. Biomass 2: 412-425.  https://doi.org/10.3390/biomass2040026
  26. Sun Z, Ramsay JA, Guay M, Ramsay BA. 2007. Carbon-limited fed-batch production of medium-chain-length polyhydroxyalkanoates from nonanoic acid by Pseudomonas putida KT2440. Appl. Microbiol. Biotechnol. 74: 69-77.  https://doi.org/10.1007/s00253-006-0655-4
  27. Vega-Castro O, Contreras-Calderon J, Leon E, Segura A, Arias M, Perez L, et al. 2016. Characterization of a polyhydroxyalkanoate obtained from pineapple peel waste using Ralsthonia eutropha. J. Biotechnol. 231: 232-238.  https://doi.org/10.1016/j.jbiotec.2016.06.018
  28. Chanasit W, Sueree L, Hodgson B, Umsakul K. 2014. The production of poly (3-hydroxybutyrate)[P (3HB)] by a newly isolated Bacillus sp. ST1C using liquid waste from biodiesel production. Annal. Microbiol. 64: 1157-1166.  https://doi.org/10.1007/s13213-013-0755-1
  29. Brandt-Talbot A, Gschwend FJ, Fennell PS, Lammens TM, Tan B, Weale J, et al. 2017. An economically viable ionic liquid for the fractionation of lignocellulosic biomass. Green Chem. 19: 3078-3102.  https://doi.org/10.1039/C7GC00705A
  30. Maarof NA. 2014. Production of poly(3-hydroxybutyrate) from pineapple waste. UMP. http://umpir.ump.edu.my/id/eprint/9158. 
  31. Palachum W, Choorit W, Chisti Y. 2021. Nutritionally enhanced probioticated whole pineapple juice. Fermentation 7: 178. 
  32. Raphael BB, Adelaja O. 2020. Production of poly-β-hydroxybutyric acid (PHB) by Bacillus cereus on pineapple peels. GSC Adv. Res. Rev. 4: 024-030.  https://doi.org/10.30574/gscarr.2020.4.1.0055
  33. dos Santos AJ, Oliveira Dalla Valentina LV, Hidalgo Schulz AA, Tomaz Duarte MA. 2017. From obtaining to degradation of PHB: material properties. Part I. Ingenieria y ciencia. 13: 269-298.  https://doi.org/10.17230/ingciencia.13.26.10
  34. Hernandez-Nunez E, Martinez-Gutierrez CA, Lopez-Cortes A, Aguirre-Macedo ML, Tabasco-Novelo C, Gonzalez-Diaz MO, et al. 2019. Physico-chemical characterization of poly (3-hydroxybutyrate) produced by Halomonas salina, isolated from a hypersaline microbial mat. J. Polym. Environ. 27: 1105-1111. https://doi.org/10.1007/s10924-019-01417-y