Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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The Synergism of Human Lactobacillaceae and Inulin Decrease Hyperglycemia via Regulating the Composition of Gut Microbiota and Metabolic Profiles in db/db Mice

  • Peifan Li (College of Biochemical Engineering, Beijing Union University) ;
  • Tong Tong (College of Biochemical Engineering, Beijing Union University) ;
  • Yusong Wu (College of Biochemical Engineering, Beijing Union University) ;
  • Xin Zhou (College of Biochemical Engineering, Beijing Union University) ;
  • Michael Zhang (Department of Physics and Astronomy, University of Manitoba) ;
  • Jia Liu (Internal Trade Food Science and Technology (Beijing) Co., Ltd) ;
  • Yongxin She (Institute of Quality Standard and Testing Technology for Agro-Products, Chinese Academy of Agricultural Science) ;
  • Zuming Li (College of Biochemical Engineering, Beijing Union University) ;
  • Yongli Li (Henan Provincial People's Hospital, People's Hospital of Zhengzhou University)
  • Received : 2023.04.24
  • Accepted : 2023.08.14
  • Published : 2023.12.28
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Abstract

This study aimed to evaluate the effects of Limosilactobacillus fermentum and Lactiplantibacillus plantarum isolated from human feces coordinating with inulin on the composition of gut microbiota and metabolic profiles in db/db mice. These supplements were administered to db/db mice for 12 weeks. The results showed that the Lactobacillaceae coordinating with inulin group (LI) exhibited lower fasting blood glucose levels than the model control group (MC). Additionally, LI was found to enhance colon tissue and increase the levels of short-chain fatty acids. 16S rRNA sequencing revealed that the abundance of Corynebacterium and Proteus, which were significantly increased in the MC group compared with NC group, were significantly decreased by the treatment of LI that also restored the key genera of the Lachnospiraceae_NK4A136_group, Lachnoclostridium, Ruminococcus_gnavus_group, Desulfovibrio, and Lachnospiraceae_UCG-006. Untargeted metabolomics analysis showed that lotaustralin, 5-hydroxyindoleacetic acid, and 13(S)-HpODE were increased while L-phenylalanine and L-tryptophan were decreased in the MC group compared with the NC group. However, the intervention of LI reversed the levels of these metabolites in the intestine. Correlation analysis revealed that Lachnoclostridium and Ruminococcus_gnavus_group were negatively correlated with 5-hydroxyindoleacetic acid and 13(S)-HpODE, but positively correlated with L-tryptophan. 13(S)-HpODE was involved in the "linoleic acid metabolism". L-tryptophan and 5-hydroxyindoleacetic acid were involved in "tryptophan metabolism" and "serotonergic synapse". These findings suggest that LI may alleviate type 2 diabetes symptoms by modulating the abundance of Ruminococcus_gnavus_group and Lachnoclostridium to regulate the pathways of "linoleic acid metabolism", "serotonergic synapse", and" tryptophan metabolism". Our results provide new insights into prevention and treatment of type 2 diabetes.

Keywords

Acknowledgement

This work was supported by the [Natural Science Foundation of Beijing Municipality] under Grant [6173033]; [Academic Research Projects of Beijing Union University] under Grant [ZK70202003]; [Sino Canada health engineering research institute cooperation projects] under Grant [50304JA1901]; [Internal trade food science and technology (Beijing) Co., Ltd cooperation projects] under Grant [202116].

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