Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Lipase Production by Limtongozyma siamensis, a Novel Lipase Producer and Lipid Accumulating Yeast

  • Varunya Sakpuntoon (Department of Microbiology, Faculty of Science, Kasetsart University) ;
  • Savitree Limtong (Department of Microbiology, Faculty of Science, Kasetsart University) ;
  • Nantana Srisuk (Department of Microbiology, Faculty of Science, Kasetsart University)
  • Received : 2023.04.04
  • Accepted : 2023.06.26
  • Published : 2023.11.28
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Abstract

Lipase is a well-known and highly in-demand enzyme. During the last decade, several lipase optimization studies have been reported. However, production costs have always been a bottleneck for commercial-scale microbial enzyme production. This research aimed to optimize the conditions for lipase production by Limtongozyma siamensis DMKU-WBL1-3 via a One-Factor-At-a-Time (OFAT) approach combined with statistical methods while using a low-cost substrate. Results suggest that low-cost substrates can be substituted for all media components. An optimal medium was found, using response surface methodology (RSM) and central composite design (CCD), to consist of 0.50% (w/v) sweet whey, 0.40% (w/v) yeast extract (food grade), and 2.50% (v/v) palm oil with the medium pH adjusted to 4 under shaking flask cultivation. From an economic point of view, this work was successful in reducing production costs while increasing lipase productivity. The medium costs were reduced by 87.5% of the original cost while lipase activity was increased by nearly 6-fold. Moreover, lipase production was further studied in a 2-L stirred-tank fermentor. Its activity was 1,055.6 ± 0.0 U/ml when aeration and agitation rates were adjusted to 1 vvm and 170 rpm, respectively. Interestingly, under this optimal lipase production, the yeast showed accumulated lipids inside the cells. The primary fatty acid is a monounsaturated fatty acid (MUFA) that is typically linked to health benefits. This study hence reveals promising lipase production and lipid accumulation by L. siamensis DMKU-WBL1-3 that are worthy of further study.

Keywords

Acknowledgement

This work was supported by the Thailand Research Fund through the Royal Golden Jubilee PhD program grant number PHD/0070/2560 to Varunya Sakpuntoon. We would like to thank UGSAS-GU via the "Microbiology Laboratory Station for IC - GU12" at Kasetsart University and International SciKU Branding (ISB), Faculty of Science, Kasetsart University.

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