Journal of Integrative Natural Science (통합자연과학논문집)

The Basic Science Institute Chosun University (조선대학교 기초과학연구원)
권호 표지
DOI QR코드

DOI QR Code

Male Reproductive System of Leucosia Anatum: Histological and Histochemical Analysis

  • Pothiappan Kumarasamy (P.G. and Research Department of Zoology, Khadir Mohideen College, Affiliated to Bharathidasan University) ;
  • Anbazhagan Pugazhendi (P.G. and Research Department of Zoology, Khadir Mohideen College, Affiliated to Bharathidasan University) ;
  • Kannayiram Muthukumaravel (P.G. and Research Department of Zoology, Khadir Mohideen College, Affiliated to Bharathidasan University) ;
  • Kumara Perumal Pradhoshini (P.G. and Research Department of Zoology, Khadir Mohideen College, Affiliated to Bharathidasan University) ;
  • Viswambaram Ganapiriya (P.G. and Research Department of Zoology, Khadir Mohideen College, Affiliated to Bharathidasan University) ;
  • Mohamed Saiyad Musthafa (Unit of Research in Radiation Biology & Environmental Radioactivity (URRBER), P.G & Research Department of Zoology, The New College (Autonomous), Affiliated to University of Madras) ;
  • Munawar Suhail Ahmed (Unit of Research in Radiation Biology & Environmental Radioactivity (URRBER), P.G & Research Department of Zoology, The New College (Autonomous), Affiliated to University of Madras)
  • Received : 2022.11.30
  • Accepted : 2023.03.16
  • Published : 2023.04.10
Download PDF
⟨ Previous Next ⟩

Abstract

Leucosia anatum, also known as the pebble crab, is a species of crab that belongs to the family Leucosiidae. Crabs are the most conspicuous and abundant components of the epibenthic macrofauna of the mangrove ecosystems. The present investigation was carried out with the objective of enlightening the reproductive system of L.anatum through histological and histochemical studies. Histological analysis of the epithelial cells of vas deferens, the luminal size and shape of the vas deferens was carried out, since the spermatophore material is secreted and molded by the vas deferens. Histochemical analysis of the blood plasma, testis, proximal vas deferens, middle vas deferens, distal vas deferens, seminal plasma and spermatophore was done to reveal the possible transformation of their secretory materials into the spermatophoric components. Biochemical components present in the blood plasma, testis, proximal vas deferens (PVD), median vas deferens (MVD) and distal vas deferens (DVD) regions seminal plasma and spermatophore of pebble crab were analyzed to find out the quantity of free sugars, proteins, and fats. Thus the current study focuses on the histological and histochemical analysis of Testis and Vas Deferens and their secretions in L.anatum.

Keywords

References

  1. Barnes,H and J. Blackstock. 1974. Biochemical composition of the seminal plasma of the Cirriped Balanus balanus (L) with particular respect to free amino acids and proteins. J. Exp. Mar. Biol. Ecol. 16: 47-85. https://doi.org/10.1016/0022-0981(74)90072-0
  2. Beninger,P.G.,R.W.Elner, T.P.Foyle, and P.H. Odense. 1988. Funtional analtomy of the male reproductive system and the female spermatheca in the snow crab Chionoecetes opilio (O.Fabricius) (Decapoda: Majidae) and a hypothesis for fertilization-J.Crust.. Biol., 8: 322-332.
  3. Casselman, W. G. B. 1954. Acetylated Sudan Black B as a more specific histo Chemical reagent for lipids. Quart. J. Micro. Sci., 95 (3) : 321-322.
  4. Castilho,GG, A.Ostrensky, M.R.Pie and W.A.Boeger, 2008. Morphology and histologyof the male reproductive system of the mangrove land crab Ucides cordatus(L) (Crustacea, Brachyura, Ocypodidae). Acta Zoologica(Stockholm) 89: 157-161. https://doi.org/10.1111/j.1463-6395.2007.00304.x
  5. Cronin, L.E. 1947. Anatomy and histology of the male reproductive system of Callinectussapidus (Rathbun) J. Morph. 81 : 209-240. https://doi.org/10.1002/jmor.1050810205
  6. Deivasigamani, B., & Subramanian, V. (2016). Applications of immunostimulants in aquaculture: a review. Int. J. Curr. Microbiol. App. Sci, 5(9), 447-453. https://doi.org/10.20546/ijcmas.2016.509.048
  7. Erkan, M., Tunali, Y., Balkis, H., Oliveira, E., 2009. Morphology of testis and vas deferens in the xanthoid crab, Eriphia verrucosa (Forskal,1775) (Decapoda: Brachyura). J. Crust. Biol. 29, 458-465. https://doi.org/10.1651/08-3114.1
  8. Garcia, T. M. and Silva, J. R. F. (2006), Testis and VasDeferens of the Red-clawed Mangrove tree crab(Goniopsis cruentata (Latereille,1803). BrazilianArchives of Biology and Technology 49 (2), 339-345.
  9. Gillikin, D.P., Kamanu, C.P., 2005. Burrowing in the east African mangrove crab Chiromantes ortmanni (Crosnier, 1965) (Decapoda: Brachyura: Sesarmidae), Crustaceana, 78(10), 1273-1275. https://doi.org/10.1163/156854005775903618
  10. Gurr, E. 1958. Methods of Analytical Histology and Histochemistry. Leonard Hill Books Ltd. London.
  11. Haley, S. R. 1984. Spermatogenesis and spermatophore production in the Hawaiian red Lobster Enoploetopus occidentalis (Randall) (Crustacea, Nephropidae). J. Morphol. 180 : 181-193. https://doi.org/10.1002/jmor.1051800303
  12. Hinch, GW. and Mc Night, CE., 1988. The vas deferens of Spanish lobster, Scyllarus chacei. Journal of Invertebrate Reproduction and Development, 13 (33) : 267-280. https://doi.org/10.1080/01688170.1988.10553497
  13. Hinsch, GW. and Walker, MH., 1974. The vas deferens of the spider crab, Libinia emarginata. Journal of Morphology, 143,(1): 1-19. https://doi.org/10.1002/jmor.1051430102
  14. Hotchkiss, R.D., 1948. A microchemical reaction resulting in the staining of polysaccharides structure in fixed tissue preparations. Arch. Biochem. 16(1), 131-141.
  15. Johnson, PT., 1980. Histology of the blue crab Callinectes sapidus: a model for Decapoda. New York: Praeger Publishers. 440 p.
  16. Kalyanram, A.J. and Chandran, M.R. 1982. The occurrence of the male accessory sex gland in the land crab, Cardisoma carnifex(Herbst). In: Profress in Invert. Reprod. & Aquacult. (Proceedings of the First All India Symposium on Inverterbrate Reproduction) (Edn). Subramonian, T. and Varadarajan, S pp.61-67, New Century Printers, Madras, India.
  17. Kumarasamy.P, 2001. A study of the malereproductive system and spermatophore formation in the marine hermit crab Diogenes costatus(Henderson) and the estuarine hermit crab Clibanarius infraspinatus(Hilgendorf). Ph.D., Thesis, Bharathidasan University, Tamilnadu.
  18. Leite, M. M. L. (2002), Estudo morfologicodo sistema reprodutor masculine do caranguejo-uca Ucides cordats Linnaeus, 1763 (Crustacea: Decapoda: Ocypodidae). 66 ff. Monografia, Universidade Federal do Ceara, Brasil.
  19. Malek, S. R. A. and Baawab, F. M. 1971. Tanning in the spermatophore of a crustacean (Penaeus trisulcatus). Experientia(Basel). 27 : 1098.
  20. Manjon-Cabeza, ME. and Raso, JEG., 2000. Morphological reproductive aspects of males of Diogenes pugilator (Roux, 1829) (Crustacea, Decapoda, Anomura) from southern Spain. Sarsia, vol. 85, p. 195-202. https://doi.org/10.1080/00364827.2000.10414572
  21. Martin, G. G., Herzig, C. and Narimatsu, G. 1987. Fine structure and histo Chemistry of the freshly extruded and hardened spermatophore of the spiny lobster Panulirus interruptus. J. Morphol. 192 : 237-246. https://doi.org/10.1002/jmor.1051920306
  22. Matthews, D.C. 1953. The development of the pedunculate spermatophore of a hermit crab Dardanus asper (De Hann). Pac. Sci., 7: 255-266.
  23. Mazia, D. Brewer, P. A. and Alfert, H. 1953. The cytochemical staining and Measurement of protein with mercuric bromophenol blue. Biol. Bull., 104 (1) : 57-67. https://doi.org/10.2307/1538691
  24. Mouchet, S. 1931. Spermatophores des crustaces decapodes, asnomures et Brachyures et castration parasitaire chez quelques pagures. Sta. oceanogr. De Salammbo, Ann. 6 : 1-2003.
  25. Pearse, A. G. E. 1968. Histochemistry Theoretical and Applied, Vol. I. 3rd Edn.. Churchill, London.
  26. Radha,T and T.Subramoniam, 1985. Origin and nature of spermatophoric mass of the spiny lobster Panulirus homarus. Mar. Biol. 86: 13-19. https://doi.org/10.1007/BF00392575
  27. Ragunath, C., & Ramasubramanian, C. (2021). Dietary Effect of Padina boergesenii on Growth, Immune Response and Disease Resistance against Pseudomonas aeruginosa in Cirrhinus mrigala.
  28. Robertson, A.I., 1986. Leaf-burying crabs: their influence on energy flow and export from mixed mangrove forests (Rhizophora sps.) in Northeastern Australia. J. Exp. Mar. Biol. Ecol., 102, 237-248. https://doi.org/10.1016/0022-0981(86)90179-6
  29. Ryan, E. P. 1967. Structure and function of the reproductive system of the crab Portunus sanquindentus (Herbst). (Brachyura, Portunidae). I. The male system. Marine Biol. Assoc. Ind. Symp. Seri. 2 : 506-521.
  30. Sasikala, S. L. and Subramoniam, T. 1987. On the occurrence of acid mucopoly saccharides in the spermatophores of two marine penaeid prawns, Penaeus indicus (Milne Edwards) and Metapenaeus monoceros (Fabricius) (Crustacea : Nacrura). J. Exp. Mar. Biol. S. Ecol. 113: 145-153. https://doi.org/10.1016/0022-0981(87)90161-4
  31. Siddiqui, M.Z.H. and M. Zafar (2002). Crabs in the Chakaria Sunderban area of Bangladesh. J. NOAMI,19: 61-77.
  32. Subramoniam T. 1984. Spermatophore formation in two intertidalcrabs Albunea symnista and Emerita asiatica (Decapoda: Anomura). Biol Bull Woods Hole 166:78-95. https://doi.org/10.2307/1541432
  33. Tran, N.D.M., Lee, D., Kim, W., Phan, D.D., Luong, D.T., 2018. Species compositionand distribution of brachyuran crabs in Duyen Hai town, Tra Vinh province. Vietnam Journal of Science, Technology and Engineering, 60(4), 39-44. https://doi.org/10.31276/VJSTE.60(4).39-44
  34. Uma, K 1982. Studies on the seminal secretions of Portunid crab Scylla serrata (Forskal) (BRachyura: Crustacea) , Ph.D Thesis, University of Madras, India.1-170
  35. Uma,K., andT. Subramoniam, 1.979.a, Histochemical characteristics of spermatophorelayers of Scylla serrata (ForskAl)(Decapoda: Portunidae). Int. J. Invertebr. Reprod. 1: 31-41. University, pp. 112.