The Plant Pathology Journal

한국식물병리학회 (The Korean Society of Plant Pathology)
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Identification and Characterization of Diplodia parva and Diplodia crataegicola Causing Black Rot of Chinese Quince

  • Sungmun Kwon (Department of Plant Medicals, Andong National University) ;
  • Jungyeon Kim (Department of Plant Medicals, Andong National University) ;
  • Younmi Lee (Department of Plant Medicals, Andong National University) ;
  • Kotnala Balaraju (Agricultural Science & Technology Research Institute, Andong National University) ;
  • Yongho Jeon (Department of Plant Medicals, Andong National University)
  • 투고 : 2023.03.09
  • 심사 : 2023.05.04
  • 발행 : 2023.06.01
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초록

Fungal isolates from infected Chinese quince trees were found to cause black rot in Yeongcheon, Gyeongsangbuk Province, Korea. The quince leaves withered and turned reddish-brown and fruits underwent black mummification. To elucidate the cause of these symptoms, the pathogen was isolated from infected leaf and fruit tissues on potato dextrose agar and Levan media. Several fungal colonies forming a fluffy white or dark gray mycelium and two types of fungi forming an aerial white mycelium, growing widely at the edges, were isolated. Microscopic observations, investigation of fungal growth characteristics on various media, and molecular identification using an internal transcribed spacer, β-tubulin, and translation elongation factor 1-α genes were performed. The fungal pathogens were identified as Diplodia parva and Diplodia crataegicola. Pathogenicity tests revealed that the pathogen-inoculated fruits exhibited a layered pattern, turning brown rotting; leaves showed circular brown necrotic lesions. The developed symptoms were similar to those observed in the field. Fungal pathogens were reisolated to fulfill Koch's postulates. Apples were inoculated with fungal pathogens to investigate the host range. Strong pathogenicity was evident in the fruits, with browning and rotting symptoms 3 days after inoculation. To determine pathogen control, a fungicidal sensitivity test was conducted using four registered fungicides. Thiophanate-methyl, propineb, and tebuconazole inhibited the mycelial growth of pathogens. To the best of our knowledge, this is the first report on the isolation and identification of the fungal pathogens D. parva and D. crataegicola from infected fruits and leaves of Chinese quince, causing black rot disease in Korea.

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과제정보

This work was supported by the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry (IPET) through the Technology Commercialization Support Program, funded by the Ministry of Agriculture, Food and Rural Affairs (MAFRA) (122039-02).

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