Animal Bioscience

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Genome wide association study for growth in Pakistani dromedary camels using genotyping-by-sequencing

  • Sajida Sabahat (Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences) ;
  • Asif Nadeem (Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences) ;
  • Rudiger Brauning (AgResearch, Invermay Agricultural Centre) ;
  • Peter C. Thomson (Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences) ;
  • Mehar S. Khatkar (The University of Sydney, Sydney School of Veterinary Science, University of Sydney)
  • Received : 2022.05.02
  • Accepted : 2022.10.28
  • Published : 2023.07.01
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Abstract

Objective: Growth performance and growth-related traits have a crucial role in livestock due to their influence on productivity. This genome-wide association study (GWAS) in Pakistani dromedary camels was conducted to identify single nucleotide polymorphisms (SNPs) associated with growth at specific camel ages, and for selected SNPs, to investigate in detail how their effects change with increasing camel age. This is the first GWAS conducted on dromedary camels in this region. Methods: Two Pakistani breeds, Marecha and Lassi, were selected for this study. A genotyping-by-sequencing method was used, and a total of 65,644 SNPs were identified. For GWAS, weight records data with several body weight traits, namely, birthweight, weaning weight, and weights of camels at 1, 2, 4, and 6 years of age were analysed by using model-based growth curve analysis. Age-specific weight data were analysed with a linear mixed model that included fixed effects of SNP genotype as well as sex. Results: Based on the q-value method for false discovery control, for Marecha camels, five SNPs at q<0.01 and 96 at q<0.05 were significantly associated with the weight traits considered, while three (q<0.01) and seven (q<0.05) SNP associations were identified for Lassi camels. Several candidate genes harbouring these SNP were discovered. Conclusion: These results will help to better understand the genetic architecture of growth including how these genes are expressed at different phases of their life. This will serve to lay the foundations for applied breeding programs of camels by allowing the genetic selection of superior animals.

Keywords

Acknowledgement

The assistance of Dr Ghulam Yasin and Dr Shahid Nabeel from the Camel Breeding & Research Station Rakh Mahni, Bhakkar, and farmers in District Lasbela, Baluchistan, are gratefully acknowledged for help with data and sample collection. We also thank Dr Ken Dodds and colleagues from AgResearch, NZ for assistance with GBS processing. SS was supported by the Higher Education Commission of Pakistan, for an IRSIP Research Fellowship to undertake part of this research at The University of Sydney.

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