The Journal of Korean Medicine (대한한의학회지)

The Society of Korean Medicine (대한한의학회)
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Protective Effect of Betula Platyphylla on Ultraviolet B-irradiated HaCaT Keratinocytes

화피(樺皮) 에탄올 추출물의 Ultraviolet B로 자극한 피부 각질 세포 보호 작용

  • Hag Soon Choi (Department of Third Medicine, Professional Graduate School of Korean Medicine, Wonkwang University ) ;
  • Hyun Joo Kim (Department of Third Medicine, Professional Graduate School of Korean Medicine, Wonkwang University ) ;
  • Hark Song Lee (Department of Korean Medicine Rehabilitation, College of Korean Medicine, Wonkwang University) ;
  • Seung Won Paik (Department of Korean Medicine Rehabilitation, College of Korean Medicine, Wonkwang University) ;
  • Ji Eun Kim (Department of Korean Medicine Rehabilitation, College of Korean Medicine, Wonkwang University) ;
  • Yung Sun Song (Department of Third Medicine, Professional Graduate School of Korean Medicine, Wonkwang University )
  • 최학순 (원광대학교 한의학전문대학원 제3의학과) ;
  • 김현주 (원광대학교 한의학전문대학원 제3의학과) ;
  • 이학송 (원광대학교 한의과대학 한방재활의학교실) ;
  • 백승원 (원광대학교 한의과대학 한방재활의학교실) ;
  • 김지은 (원광대학교 한의과대학 한방재활의학교실) ;
  • 송용선 (원광대학교 한의학전문대학원 제3의학과)
  • Received : 2023.04.17
  • Accepted : 2023.05.10
  • Published : 2023.06.01
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Abstract

Objectives: Betula Platyphylla(BP) has been used as a analgesic, anti-microbial, anti-oxidant drug in Eastern Asia. However, it is still unknown whether BP ethanol extract could exhibit the inhibitory activities against ultraviolet B(UVB)-induced skin injury on human keratinocytes, HaCaT cells. This study was aimed to investigate the protective activity of BP ethanol extract on UVB-irradiated skin injury in HaCaT cells. Methods: The skin injury model of HaCaT cells was established under UVB stimulation. HaCaT keratinocyte cells were pre-treated with BP ethanol extract for 1 h, and then stimulated with UVB. Then, the cells were harvested to measure the cell viability, production of reactive oxygen species(ROS), pro-inflammatory cytokines such as interleukin(IL) 1-beta, IL-6, and tumor necrosis factor(TNF)-𝛼, hyaluronidase, type 1 collagen, matrix metalloproteinase(MMP)s. In addition, we examined the mitogen activated protein kinases(MAPKs) and inhibitory kappa B alpha(I𝜅;-B𝛼) as inhibitory mechanisms of BP ethanol extract. Results: The treatment of BP ethanol extract inhibited the UVBinduced cell death and ROS production in HaCaT cells. BP ethanol extract treatment inhibited the UVB-induced increase of IL-1beta, IL-6, and TNF-𝛼. BP ethanol extract treatment inhibited the increase of hyaluronidase, MMP and decrease of collagen. BP ethanol extract treatment inhibited the activation of MAPKs and the degradation of I𝜅-B𝛼. Conclusions: Our result suggest that treatment of BP ethanol extract could inhibit the UVB-induced skin injury via deactivation of MAPKs and nuclear factor kappa B(NF-𝜅B) in HaCaT cells. This study could suggest that BP ethanol extract could be a beneficial agent to prevent skin damage or inflammation.

Keywords

Acknowledgement

본 연구는 2022년도 원광대학교 교비 지원에 의하여 연구되었음.

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